Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection.
Trichinella spiralis muscle stage larvae (mL1) produce excretory-secreted products (ESPs), a complex mixture of protein, which are believed to be important for establishing or maintaining an infection niche within skeletal muscle and the intestine. Studies of both whole ESPs and individual cloned pr...
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Online Access: | https://doi.org/10.1371/journal.pntd.0008842 https://doaj.org/article/24bb9c61d84a4d1eba2de74d3e2572c8 |
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ftdoajarticles:oai:doaj.org/article:24bb9c61d84a4d1eba2de74d3e2572c8 2023-05-15T15:07:42+02:00 Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. Mellina T Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E Selkirk Tracey J Lamb David B Guiliano 2020-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008842 https://doaj.org/article/24bb9c61d84a4d1eba2de74d3e2572c8 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008842 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008842 https://doaj.org/article/24bb9c61d84a4d1eba2de74d3e2572c8 PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008842 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008842 2022-12-31T07:48:33Z Trichinella spiralis muscle stage larvae (mL1) produce excretory-secreted products (ESPs), a complex mixture of protein, which are believed to be important for establishing or maintaining an infection niche within skeletal muscle and the intestine. Studies of both whole ESPs and individual cloned proteins have shown that some ESPs are potent immunogens capable of eliciting protective immune responses. Here we describe two novel proteins, Secreted from Muscle stage Larvae SML-4 and SML-5 which are 15 kDa and 12 kDa respectively. The genes encoding these proteins are highly conserved within the Trichinellids, are constituents of mL1 ESP and localized in the parasite stichosome. While SML-5 is only expressed in mL1 and early stages of adult nematode development, SML-4 is a tyvosylated glycoprotein also produced by adult nematodes, indicating it may have a function in the enteral phase of the infection. Vaccination with these proteins resulted in an impaired establishment of adult stages and consequently a reduction in the burden of mL1 in BALB/c mice. This suggests that both proteins may be important for establishment of parasite infection of the intestine and are prophylactic vaccine candidates. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 11 e0008842 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mellina T Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E Selkirk Tracey J Lamb David B Guiliano Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trichinella spiralis muscle stage larvae (mL1) produce excretory-secreted products (ESPs), a complex mixture of protein, which are believed to be important for establishing or maintaining an infection niche within skeletal muscle and the intestine. Studies of both whole ESPs and individual cloned proteins have shown that some ESPs are potent immunogens capable of eliciting protective immune responses. Here we describe two novel proteins, Secreted from Muscle stage Larvae SML-4 and SML-5 which are 15 kDa and 12 kDa respectively. The genes encoding these proteins are highly conserved within the Trichinellids, are constituents of mL1 ESP and localized in the parasite stichosome. While SML-5 is only expressed in mL1 and early stages of adult nematode development, SML-4 is a tyvosylated glycoprotein also produced by adult nematodes, indicating it may have a function in the enteral phase of the infection. Vaccination with these proteins resulted in an impaired establishment of adult stages and consequently a reduction in the burden of mL1 in BALB/c mice. This suggests that both proteins may be important for establishment of parasite infection of the intestine and are prophylactic vaccine candidates. |
format |
Article in Journal/Newspaper |
author |
Mellina T Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E Selkirk Tracey J Lamb David B Guiliano |
author_facet |
Mellina T Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E Selkirk Tracey J Lamb David B Guiliano |
author_sort |
Mellina T Srey |
title |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. |
title_short |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. |
title_full |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. |
title_fullStr |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. |
title_full_unstemmed |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection. |
title_sort |
vaccination with novel low-molecular weight proteins secreted from trichinella spiralis inhibits establishment of infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008842 https://doaj.org/article/24bb9c61d84a4d1eba2de74d3e2572c8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008842 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008842 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008842 https://doaj.org/article/24bb9c61d84a4d1eba2de74d3e2572c8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008842 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
11 |
container_start_page |
e0008842 |
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1766339140813586432 |