The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage.
BACKGROUND: Mixed intestinal infections with Entamoeba histolytica, Entamoeba dispar and bacteria with exacerbated manifestations of disease are common in regions where amoebiasis is endemic. However, amoeba-bacteria interactions remain largely unexamined. METHODOLOGY: Trophozoites of E. histolytica...
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ftdoajarticles:oai:doaj.org/article:24b602ddb2574608b6ad88acd82001c9 2023-05-15T15:12:58+02:00 The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage. José Manuel Galván-Moroyoqui M Del Carmen Domínguez-Robles Elizabeth Franco Isaura Meza 2008-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000266 https://doaj.org/article/24b602ddb2574608b6ad88acd82001c9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2447883?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000266 https://doaj.org/article/24b602ddb2574608b6ad88acd82001c9 PLoS Neglected Tropical Diseases, Vol 2, Iss 7, p e266 (2008) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2008 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000266 2022-12-31T00:08:52Z BACKGROUND: Mixed intestinal infections with Entamoeba histolytica, Entamoeba dispar and bacteria with exacerbated manifestations of disease are common in regions where amoebiasis is endemic. However, amoeba-bacteria interactions remain largely unexamined. METHODOLOGY: Trophozoites of E. histolytica and E. dispar were co-cultured with enteropathogenic bacteria strains Escherichia coli (EPEC), Shigella dysenteriae and a commensal Escherichia coli. Amoebae that phagocytosed bacteria were tested for a cytopathic effect on epithelial cell monolayers. Cysteine proteinase activity, adhesion and cell surface concentration of Gal/GalNAc lectin were analyzed in amoebae showing increased virulence. Structural and functional changes and induction of IL-8 expression were determined in epithelial cells before and after exposure to bacteria. Chemotaxis of amoebae and neutrophils to human IL-8 and conditioned culture media from epithelial cells exposed to bacteria was quantified. PRINCIPAL FINDINGS: E. histolytica digested phagocytosed bacteria, although S. dysenteriae retained 70% viability after ingestion. Phagocytosis of pathogenic bacteria augmented the cytopathic effect of E. histolytica and increased expression of Gal/GalNAc lectin on the amoebic surface and increased cysteine proteinase activity. E. dispar remained avirulent. Adhesion of amoebae and damage to cells exposed to bacteria were increased. Additional increases were observed if amoebae had phagocytosed bacteria. Co-culture of epithelial cells with enteropathogenic bacteria disrupted monolayer permeability and induced expression of IL-8. Media from these co-cultures and human recombinant IL-8 were similarly chemotactic for neutrophils and E. histolytica. CONCLUSIONS: Epithelial monolayers exposed to enteropathogenic bacteria become more susceptible to E. histolytica damage. At the same time, phagocytosis of pathogenic bacteria by amoebae further increased epithelial cell damage. SIGNIFICANCE: The in vitro system presented here provides evidence that the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 2 7 e266 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 José Manuel Galván-Moroyoqui M Del Carmen Domínguez-Robles Elizabeth Franco Isaura Meza The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Mixed intestinal infections with Entamoeba histolytica, Entamoeba dispar and bacteria with exacerbated manifestations of disease are common in regions where amoebiasis is endemic. However, amoeba-bacteria interactions remain largely unexamined. METHODOLOGY: Trophozoites of E. histolytica and E. dispar were co-cultured with enteropathogenic bacteria strains Escherichia coli (EPEC), Shigella dysenteriae and a commensal Escherichia coli. Amoebae that phagocytosed bacteria were tested for a cytopathic effect on epithelial cell monolayers. Cysteine proteinase activity, adhesion and cell surface concentration of Gal/GalNAc lectin were analyzed in amoebae showing increased virulence. Structural and functional changes and induction of IL-8 expression were determined in epithelial cells before and after exposure to bacteria. Chemotaxis of amoebae and neutrophils to human IL-8 and conditioned culture media from epithelial cells exposed to bacteria was quantified. PRINCIPAL FINDINGS: E. histolytica digested phagocytosed bacteria, although S. dysenteriae retained 70% viability after ingestion. Phagocytosis of pathogenic bacteria augmented the cytopathic effect of E. histolytica and increased expression of Gal/GalNAc lectin on the amoebic surface and increased cysteine proteinase activity. E. dispar remained avirulent. Adhesion of amoebae and damage to cells exposed to bacteria were increased. Additional increases were observed if amoebae had phagocytosed bacteria. Co-culture of epithelial cells with enteropathogenic bacteria disrupted monolayer permeability and induced expression of IL-8. Media from these co-cultures and human recombinant IL-8 were similarly chemotactic for neutrophils and E. histolytica. CONCLUSIONS: Epithelial monolayers exposed to enteropathogenic bacteria become more susceptible to E. histolytica damage. At the same time, phagocytosis of pathogenic bacteria by amoebae further increased epithelial cell damage. SIGNIFICANCE: The in vitro system presented here provides evidence that the ... |
format |
Article in Journal/Newspaper |
author |
José Manuel Galván-Moroyoqui M Del Carmen Domínguez-Robles Elizabeth Franco Isaura Meza |
author_facet |
José Manuel Galván-Moroyoqui M Del Carmen Domínguez-Robles Elizabeth Franco Isaura Meza |
author_sort |
José Manuel Galván-Moroyoqui |
title |
The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage. |
title_short |
The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage. |
title_full |
The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage. |
title_fullStr |
The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage. |
title_full_unstemmed |
The interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage. |
title_sort |
interplay between entamoeba and enteropathogenic bacteria modulates epithelial cell damage. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2008 |
url |
https://doi.org/10.1371/journal.pntd.0000266 https://doaj.org/article/24b602ddb2574608b6ad88acd82001c9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 2, Iss 7, p e266 (2008) |
op_relation |
http://europepmc.org/articles/PMC2447883?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000266 https://doaj.org/article/24b602ddb2574608b6ad88acd82001c9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000266 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
2 |
container_issue |
7 |
container_start_page |
e266 |
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1766343580999221248 |