Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants
Objective: To screen, isolate and optimize anti-white spot syndrome virus (WSSV) drug derived from various terrestrial plants and to evaluate the efficacy of the same in host–pathogen interaction model. Methods: Thirty plants were subjected to Soxhlet extraction using water, ethanol, methanol and he...
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ftdoajarticles:oai:doaj.org/article:24a80606af2f4c3eab0d0fb82fccadcc 2023-05-15T15:08:43+02:00 Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants Upasana Ghosh Somnath Chakraborty Thangavel Balasubramanian Punyabrata Das 2014-05-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1042 https://doaj.org/article/24a80606af2f4c3eab0d0fb82fccadcc EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115302549 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1042 https://doaj.org/article/24a80606af2f4c3eab0d0fb82fccadcc Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S118-S128 (2014) Shrimps Litopenaeus vannamei Anti–WSSV Terrestrial plants TP22C White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1042 2022-12-31T14:41:39Z Objective: To screen, isolate and optimize anti-white spot syndrome virus (WSSV) drug derived from various terrestrial plants and to evaluate the efficacy of the same in host–pathogen interaction model. Methods: Thirty plants were subjected to Soxhlet extraction using water, ethanol, methanol and hexane as solvents. The 120 plant isolates thus obtained were screened for their in vivo anti–WSSV property in Litopenaeus vannamei. The best anti–WSSV plant isolate, TP22C was isolated and further analyzed. The drug was optimized at various concentrations. Viral and immune genes were analysed using reverse transcriptase PCR to confirm the potency of the drug. Results: Seven plant isolates exhibited significant survivability in host. The drug TP22C thus formulated showed 86% survivability in host. The surviving shrimps were nested PCR negative at the end of the 15 d experimentation. The lowest concentration of TP22C required intramuscularly for virucidal property was 10 mg/mL. The oral dosage of 750 mg/kg body weight/day survived at the rate of 86%. Neither VP28 nor ie 1 was expressed in the test samples at 42nd hour and 84th hour post viral infection. Conclusions: The drug TP22C derived from Momordica charantia is a potent anti-white spot syndrome virus drug. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S118 S128 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Shrimps Litopenaeus vannamei Anti–WSSV Terrestrial plants TP22C White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Shrimps Litopenaeus vannamei Anti–WSSV Terrestrial plants TP22C White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Upasana Ghosh Somnath Chakraborty Thangavel Balasubramanian Punyabrata Das Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants |
topic_facet |
Shrimps Litopenaeus vannamei Anti–WSSV Terrestrial plants TP22C White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To screen, isolate and optimize anti-white spot syndrome virus (WSSV) drug derived from various terrestrial plants and to evaluate the efficacy of the same in host–pathogen interaction model. Methods: Thirty plants were subjected to Soxhlet extraction using water, ethanol, methanol and hexane as solvents. The 120 plant isolates thus obtained were screened for their in vivo anti–WSSV property in Litopenaeus vannamei. The best anti–WSSV plant isolate, TP22C was isolated and further analyzed. The drug was optimized at various concentrations. Viral and immune genes were analysed using reverse transcriptase PCR to confirm the potency of the drug. Results: Seven plant isolates exhibited significant survivability in host. The drug TP22C thus formulated showed 86% survivability in host. The surviving shrimps were nested PCR negative at the end of the 15 d experimentation. The lowest concentration of TP22C required intramuscularly for virucidal property was 10 mg/mL. The oral dosage of 750 mg/kg body weight/day survived at the rate of 86%. Neither VP28 nor ie 1 was expressed in the test samples at 42nd hour and 84th hour post viral infection. Conclusions: The drug TP22C derived from Momordica charantia is a potent anti-white spot syndrome virus drug. |
format |
Article in Journal/Newspaper |
author |
Upasana Ghosh Somnath Chakraborty Thangavel Balasubramanian Punyabrata Das |
author_facet |
Upasana Ghosh Somnath Chakraborty Thangavel Balasubramanian Punyabrata Das |
author_sort |
Upasana Ghosh |
title |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants |
title_short |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants |
title_full |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants |
title_fullStr |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants |
title_full_unstemmed |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants |
title_sort |
screening, isolation and optimization of anti–white spot syndrome virus drug derived from terrestrial plants |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1042 https://doaj.org/article/24a80606af2f4c3eab0d0fb82fccadcc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S118-S128 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115302549 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1042 https://doaj.org/article/24a80606af2f4c3eab0d0fb82fccadcc |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1042 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S118 |
op_container_end_page |
S128 |
_version_ |
1766340021954019328 |