Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility
Flow cytometric analysis is a useful and widely employed tool to identify immunological alterations caused by different microorganisms, including Mycobacterium tuberculosis. However, this tool can be used for several others analysis. We will discuss some applications for flow cytometry to the study...
| Published in: | Memórias do Instituto Oswaldo Cruz |
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| Main Author: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
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Instituto Oswaldo Cruz, Ministério da Saúde
2000
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| Online Access: | https://doi.org/10.1590/S0074-02762000000400008 https://doaj.org/article/2493e9a24e5a40789a11a5c9abda57d7 |
| Summary: | Flow cytometric analysis is a useful and widely employed tool to identify immunological alterations caused by different microorganisms, including Mycobacterium tuberculosis. However, this tool can be used for several others analysis. We will discuss some applications for flow cytometry to the study of M. tuberculosis, mainly on cell surface antigens, mycobacterial secreted proteins, their interaction with the immune system using inflammatory cells recovered from peripheral blood, alveolar and pleura spaces and the influence of M. tuberculosis on apoptosis, and finally the rapid determination of drug susceptibility. All of these examples highlight the usefulness of flow cytometry in the study of M. tuber-culosis infection. |
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