RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation.
Leishmania is a protozoan parasite that alternates its life cycle between the sand-fly vector and the mammalian host. This alternation involves environmental changes and leads the parasite to dynamic modifications in morphology, metabolism, cellular signaling and regulation of gene expression to all...
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ftdoajarticles:oai:doaj.org/article:246fdd34660e4d1e8c128f9711a14420 2023-05-15T15:15:17+02:00 RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation. Juliana Ide Aoki Sandra Marcia Muxel Ricardo Andrade Zampieri Maria Fernanda Laranjeira-Silva Karl Erik Müller Audun Helge Nerland Lucile Maria Floeter-Winter 2017-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006026 https://doaj.org/article/246fdd34660e4d1e8c128f9711a14420 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5678721?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006026 https://doaj.org/article/246fdd34660e4d1e8c128f9711a14420 PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0006026 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006026 2022-12-31T14:52:19Z Leishmania is a protozoan parasite that alternates its life cycle between the sand-fly vector and the mammalian host. This alternation involves environmental changes and leads the parasite to dynamic modifications in morphology, metabolism, cellular signaling and regulation of gene expression to allow for a rapid adaptation to new conditions. The L-arginine pathway in L. amazonensis is important during the parasite life cycle and interferes in the establishment and maintenance of the infection in mammalian macrophages. Host arginase is an immune-regulatory enzyme that can reduce the production of nitric oxide by activated macrophages, directing the availability of L-arginine to the polyamine pathway, resulting in parasite replication. In this work, we performed transcriptional profiling to identify differentially expressed genes in L. amazonensis wild-type (La-WT) versus L. amazonensis arginase knockout (La-arg-) promastigotes and axenic amastigotes.A total of 8253 transcripts were identified in La-WT and La-arg- promastigotes and axenic amastigotes, about 60% of them codifying hypothetical proteins and 443 novel transcripts, which did not match any previously annotated genes. Our RNA-seq data revealed that 85% of genes were constitutively expressed. The comparison of transcriptome and metabolome data showed lower levels of arginase and higher levels of glutamate-5-kinase in La-WT axenic amastigotes compared to promastigotes. The absence of arginase activity in promastigotes increased the levels of pyrroline 5-carboxylate reductase, but decreased the levels of arginosuccinate synthase, pyrroline 5-carboxylate dehydrogenase, acetylornithine deacetylase and spermidine synthase transcripts levels. These observations can explain previous metabolomic data pointing to the increase of L-arginine, citrulline and L-glutamate and reduction of aspartate, proline, ornithine and putrescine. Altogether, these results indicate that arginase activity is important in Leishmania gene expression modulation during differentiation ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 10 e0006026 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Juliana Ide Aoki Sandra Marcia Muxel Ricardo Andrade Zampieri Maria Fernanda Laranjeira-Silva Karl Erik Müller Audun Helge Nerland Lucile Maria Floeter-Winter RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leishmania is a protozoan parasite that alternates its life cycle between the sand-fly vector and the mammalian host. This alternation involves environmental changes and leads the parasite to dynamic modifications in morphology, metabolism, cellular signaling and regulation of gene expression to allow for a rapid adaptation to new conditions. The L-arginine pathway in L. amazonensis is important during the parasite life cycle and interferes in the establishment and maintenance of the infection in mammalian macrophages. Host arginase is an immune-regulatory enzyme that can reduce the production of nitric oxide by activated macrophages, directing the availability of L-arginine to the polyamine pathway, resulting in parasite replication. In this work, we performed transcriptional profiling to identify differentially expressed genes in L. amazonensis wild-type (La-WT) versus L. amazonensis arginase knockout (La-arg-) promastigotes and axenic amastigotes.A total of 8253 transcripts were identified in La-WT and La-arg- promastigotes and axenic amastigotes, about 60% of them codifying hypothetical proteins and 443 novel transcripts, which did not match any previously annotated genes. Our RNA-seq data revealed that 85% of genes were constitutively expressed. The comparison of transcriptome and metabolome data showed lower levels of arginase and higher levels of glutamate-5-kinase in La-WT axenic amastigotes compared to promastigotes. The absence of arginase activity in promastigotes increased the levels of pyrroline 5-carboxylate reductase, but decreased the levels of arginosuccinate synthase, pyrroline 5-carboxylate dehydrogenase, acetylornithine deacetylase and spermidine synthase transcripts levels. These observations can explain previous metabolomic data pointing to the increase of L-arginine, citrulline and L-glutamate and reduction of aspartate, proline, ornithine and putrescine. Altogether, these results indicate that arginase activity is important in Leishmania gene expression modulation during differentiation ... |
format |
Article in Journal/Newspaper |
author |
Juliana Ide Aoki Sandra Marcia Muxel Ricardo Andrade Zampieri Maria Fernanda Laranjeira-Silva Karl Erik Müller Audun Helge Nerland Lucile Maria Floeter-Winter |
author_facet |
Juliana Ide Aoki Sandra Marcia Muxel Ricardo Andrade Zampieri Maria Fernanda Laranjeira-Silva Karl Erik Müller Audun Helge Nerland Lucile Maria Floeter-Winter |
author_sort |
Juliana Ide Aoki |
title |
RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation. |
title_short |
RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation. |
title_full |
RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation. |
title_fullStr |
RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation. |
title_full_unstemmed |
RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation. |
title_sort |
rna-seq transcriptional profiling of leishmania amazonensis reveals an arginase-dependent gene expression regulation. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006026 https://doaj.org/article/246fdd34660e4d1e8c128f9711a14420 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0006026 (2017) |
op_relation |
http://europepmc.org/articles/PMC5678721?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006026 https://doaj.org/article/246fdd34660e4d1e8c128f9711a14420 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006026 |
container_title |
PLOS Neglected Tropical Diseases |
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11 |
container_issue |
10 |
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e0006026 |
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1766345645259489280 |