Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE)
The pressurized liquid extraction (PLE) technique was used to obtain protein extracts with antioxidant capacity from salmon muscle remains, heads, viscera, skin, and tailfins. A protein recovery percentage ≈28% was obtained for all samples except for viscera, which was ≈92%. These values represented...
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ftdoajarticles:oai:doaj.org/article:245abe0e7f8e49e791fbd77192cbce15 2023-05-15T18:09:57+02:00 Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) Beatriz de la Fuente Noelia Pallarés Houda Berrada Francisco J. Barba 2021-06-01T00:00:00Z https://doi.org/10.3390/md19060323 https://doaj.org/article/245abe0e7f8e49e791fbd77192cbce15 EN eng MDPI AG https://www.mdpi.com/1660-3397/19/6/323 https://doaj.org/toc/1660-3397 doi:10.3390/md19060323 1660-3397 https://doaj.org/article/245abe0e7f8e49e791fbd77192cbce15 Marine Drugs, Vol 19, Iss 323, p 323 (2021) pressurized liquid extraction salmon side streams peptides protein SDS-PAGE Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/md19060323 2022-12-30T22:12:00Z The pressurized liquid extraction (PLE) technique was used to obtain protein extracts with antioxidant capacity from salmon muscle remains, heads, viscera, skin, and tailfins. A protein recovery percentage ≈28% was obtained for all samples except for viscera, which was ≈92%. These values represented an increase of 1.5–4.8-fold compared to stirring extraction (control). Different SDS-PAGE profiles in control and PLE extracts revealed that extraction conditions affected the protein molecular weight distribution of the obtained extracts. Both TEAC (Trolox equivalent antioxidant capacity) and ORAC (oxygen radical antioxidant capacity) assays showed an outstanding antioxidant activity for viscera PLE extract. Through liquid chromatography coupled with electrospray ionization triple time-of-flight (nanoESI qQTOF) mass spectrometry, 137 and 67 peptides were identified in control and PLE extracts from salmon viscera, respectively None of these peptides was found among the antioxidant peptides inputted in the BIOPEP-UMP database. However, bioinformatics analysis showed several antioxidant small peptides encrypted in amino acid sequences of viscera extracts, especially GPP (glycine-proline-proline) and GAA (glycine-alanine-alanine) for PLE extracts. Further research on the relationship between antioxidant activity and specific peptides from salmon viscera PLE extracts is required. In addition, the salmon side streams studied presented non-toxic levels of As, Hg, Cd, and Pb, as well as the absence of mycotoxins or related metabolites. Overall, these results confirm the feasible use of farmed salmon processing side streams as alternative sources of protein and bioactive compounds for human consumption. Article in Journal/Newspaper Salmo salar Directory of Open Access Journals: DOAJ Articles Marine Drugs 19 6 323 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
pressurized liquid extraction salmon side streams peptides protein SDS-PAGE Biology (General) QH301-705.5 |
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pressurized liquid extraction salmon side streams peptides protein SDS-PAGE Biology (General) QH301-705.5 Beatriz de la Fuente Noelia Pallarés Houda Berrada Francisco J. Barba Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
topic_facet |
pressurized liquid extraction salmon side streams peptides protein SDS-PAGE Biology (General) QH301-705.5 |
description |
The pressurized liquid extraction (PLE) technique was used to obtain protein extracts with antioxidant capacity from salmon muscle remains, heads, viscera, skin, and tailfins. A protein recovery percentage ≈28% was obtained for all samples except for viscera, which was ≈92%. These values represented an increase of 1.5–4.8-fold compared to stirring extraction (control). Different SDS-PAGE profiles in control and PLE extracts revealed that extraction conditions affected the protein molecular weight distribution of the obtained extracts. Both TEAC (Trolox equivalent antioxidant capacity) and ORAC (oxygen radical antioxidant capacity) assays showed an outstanding antioxidant activity for viscera PLE extract. Through liquid chromatography coupled with electrospray ionization triple time-of-flight (nanoESI qQTOF) mass spectrometry, 137 and 67 peptides were identified in control and PLE extracts from salmon viscera, respectively None of these peptides was found among the antioxidant peptides inputted in the BIOPEP-UMP database. However, bioinformatics analysis showed several antioxidant small peptides encrypted in amino acid sequences of viscera extracts, especially GPP (glycine-proline-proline) and GAA (glycine-alanine-alanine) for PLE extracts. Further research on the relationship between antioxidant activity and specific peptides from salmon viscera PLE extracts is required. In addition, the salmon side streams studied presented non-toxic levels of As, Hg, Cd, and Pb, as well as the absence of mycotoxins or related metabolites. Overall, these results confirm the feasible use of farmed salmon processing side streams as alternative sources of protein and bioactive compounds for human consumption. |
format |
Article in Journal/Newspaper |
author |
Beatriz de la Fuente Noelia Pallarés Houda Berrada Francisco J. Barba |
author_facet |
Beatriz de la Fuente Noelia Pallarés Houda Berrada Francisco J. Barba |
author_sort |
Beatriz de la Fuente |
title |
Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_short |
Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_full |
Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_fullStr |
Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_full_unstemmed |
Salmon ( Salmo salar ) Side Streams as a Bioresource to Obtain Potential Antioxidant Peptides after Applying Pressurized Liquid Extraction (PLE) |
title_sort |
salmon ( salmo salar ) side streams as a bioresource to obtain potential antioxidant peptides after applying pressurized liquid extraction (ple) |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19060323 https://doaj.org/article/245abe0e7f8e49e791fbd77192cbce15 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Marine Drugs, Vol 19, Iss 323, p 323 (2021) |
op_relation |
https://www.mdpi.com/1660-3397/19/6/323 https://doaj.org/toc/1660-3397 doi:10.3390/md19060323 1660-3397 https://doaj.org/article/245abe0e7f8e49e791fbd77192cbce15 |
op_doi |
https://doi.org/10.3390/md19060323 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
6 |
container_start_page |
323 |
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1766182649225805824 |