Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis
Abstract Background Anopheles maculipennis complex, the historic vector of malaria, causes serious medical problems worldwide and exhibits different behaviours. Studying the odorant-binding proteins (OBPs), which influence the chemosensory system and behavioural responses, is essential to understand...
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ftdoajarticles:oai:doaj.org/article:2459c62763a74495a0251e61f47ff636 2023-05-15T15:14:22+02:00 Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis Mohammad Bagher Ghavami Sakineh Khoeini Navid Dinparast Djadid 2020-01-01T00:00:00Z https://doi.org/10.1186/s12936-019-3058-6 https://doaj.org/article/2459c62763a74495a0251e61f47ff636 EN eng BMC https://doi.org/10.1186/s12936-019-3058-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3058-6 1475-2875 https://doaj.org/article/2459c62763a74495a0251e61f47ff636 Malaria Journal, Vol 19, Iss 1, Pp 1-10 (2020) Anopheles maculipennis Odorant-binding protein 1 Amacobp1 gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-019-3058-6 2022-12-31T04:59:17Z Abstract Background Anopheles maculipennis complex, the historic vector of malaria, causes serious medical problems worldwide and exhibits different behaviours. Studying the odorant-binding proteins (OBPs), which influence the chemosensory system and behavioural responses, is essential to understanding the population structure and developing effective control measures against this vector. The present study was designed to identify and analyse the obp1 gene in An. maculipennis. Methods Adults of An. maculipennis sensu stricto were collected in Zanjan Province, northwest of Iran, and gDNAs of female mosquitoes were extracted. Fragments of An. maculipennis obp1 (Amacobp1) gene were amplified using degenerate and specific primers, and some of amplicons were selected for sequencing. Results Analysis of amplified products identified that the sequence of Amacobp1 gene was 1341 bp long. This gene contains three exons (5′, internal, and 3′of 160, 256, and 18 bp, respectively) and encodes 144 amino acids. The sizes of introns I and II in deduced gene are 268 and 358 nucleotides, respectively. The amino acid sequence in the C-terminal of AmacOBP1 is similar to that of major malaria vector Anopheles species. However, its N-terminal has a specific signal peptide with 19 amino acids. This peptide is conserved in different studied populations, and its sequence of amino acids shows the most variation among anopheline species. Conclusions Degenerate primers in this study are suggested for studying obp1 gene in Anopheles species. Amacobp1 gene is proposed as a molecular marker for the detection of intraspecific ecotypes and diagnosis of different species within Maculipennis Group. Moreover, the N-terminal of AmacOBP1 peptide is recommended as a molecular marker to identify the Amacobp1 expression patterns in different chemosensory organs for assessing the molecular mechanisms and developing novel behavioural disturbance agents to control An. maculipennis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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ftdoajarticles |
language |
English |
topic |
Anopheles maculipennis Odorant-binding protein 1 Amacobp1 gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Anopheles maculipennis Odorant-binding protein 1 Amacobp1 gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mohammad Bagher Ghavami Sakineh Khoeini Navid Dinparast Djadid Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis |
topic_facet |
Anopheles maculipennis Odorant-binding protein 1 Amacobp1 gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles maculipennis complex, the historic vector of malaria, causes serious medical problems worldwide and exhibits different behaviours. Studying the odorant-binding proteins (OBPs), which influence the chemosensory system and behavioural responses, is essential to understanding the population structure and developing effective control measures against this vector. The present study was designed to identify and analyse the obp1 gene in An. maculipennis. Methods Adults of An. maculipennis sensu stricto were collected in Zanjan Province, northwest of Iran, and gDNAs of female mosquitoes were extracted. Fragments of An. maculipennis obp1 (Amacobp1) gene were amplified using degenerate and specific primers, and some of amplicons were selected for sequencing. Results Analysis of amplified products identified that the sequence of Amacobp1 gene was 1341 bp long. This gene contains three exons (5′, internal, and 3′of 160, 256, and 18 bp, respectively) and encodes 144 amino acids. The sizes of introns I and II in deduced gene are 268 and 358 nucleotides, respectively. The amino acid sequence in the C-terminal of AmacOBP1 is similar to that of major malaria vector Anopheles species. However, its N-terminal has a specific signal peptide with 19 amino acids. This peptide is conserved in different studied populations, and its sequence of amino acids shows the most variation among anopheline species. Conclusions Degenerate primers in this study are suggested for studying obp1 gene in Anopheles species. Amacobp1 gene is proposed as a molecular marker for the detection of intraspecific ecotypes and diagnosis of different species within Maculipennis Group. Moreover, the N-terminal of AmacOBP1 peptide is recommended as a molecular marker to identify the Amacobp1 expression patterns in different chemosensory organs for assessing the molecular mechanisms and developing novel behavioural disturbance agents to control An. maculipennis. |
format |
Article in Journal/Newspaper |
author |
Mohammad Bagher Ghavami Sakineh Khoeini Navid Dinparast Djadid |
author_facet |
Mohammad Bagher Ghavami Sakineh Khoeini Navid Dinparast Djadid |
author_sort |
Mohammad Bagher Ghavami |
title |
Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis |
title_short |
Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis |
title_full |
Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis |
title_fullStr |
Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis |
title_full_unstemmed |
Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis |
title_sort |
molecular characteristics of odorant-binding protein 1 in anopheles maculipennis |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-019-3058-6 https://doaj.org/article/2459c62763a74495a0251e61f47ff636 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-10 (2020) |
op_relation |
https://doi.org/10.1186/s12936-019-3058-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-3058-6 1475-2875 https://doaj.org/article/2459c62763a74495a0251e61f47ff636 |
op_doi |
https://doi.org/10.1186/s12936-019-3058-6 |
container_title |
Malaria Journal |
container_volume |
19 |
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1 |
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1766344829197877248 |