The miRNA biogenesis in marine bivalves
Small non-coding RNAs include powerful regulators of gene expression, transposon mobility and virus activity. Among the various categories, mature microRNAs (miRNAs) guide the translational repression and decay of several targeted mRNAs. The biogenesis of miRNAs depends on few gene products, essenti...
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ftdoajarticles:oai:doaj.org/article:24594bec0567426da90575bb34d95bc6 2023-10-01T03:55:31+02:00 The miRNA biogenesis in marine bivalves Umberto Rosani Alberto Pallavicini Paola Venier 2016-03-01T00:00:00Z https://doi.org/10.7717/peerj.1763 https://doaj.org/article/24594bec0567426da90575bb34d95bc6 EN eng PeerJ Inc. https://peerj.com/articles/1763.pdf https://peerj.com/articles/1763/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.1763 2167-8359 https://doaj.org/article/24594bec0567426da90575bb34d95bc6 PeerJ, Vol 4, p e1763 (2016) miRNA biogenesis Mytilus galloprovincialis Crassostrea gigas Bivalves RNAi Medicine R article 2016 ftdoajarticles https://doi.org/10.7717/peerj.1763 2023-09-03T00:46:00Z Small non-coding RNAs include powerful regulators of gene expression, transposon mobility and virus activity. Among the various categories, mature microRNAs (miRNAs) guide the translational repression and decay of several targeted mRNAs. The biogenesis of miRNAs depends on few gene products, essentially conserved from basal to higher metazoans, whose protein domains allow specific interactions with dsRNA. Here, we report the identification of key genes responsible of the miRNA biogenesis in 32 bivalves, with particular attention to the aquaculture species Mytilus galloprovincialis and Crassostrea gigas. In detail, we have identified and phylogenetically compared eight evolutionary conserved proteins: DROSHA, DGCR8, EXP5, RAN, DICER TARBP2, AGO and PIWI. In mussels, we recognized several other proteins participating in the miRNA biogenesis or in the subsequent RNA silencing. According to digital expression analysis, these genes display low and not inducible expression levels in adult mussels and oysters whereas they are considerably expressed during development. As miRNAs play an important role also in the antiviral responses, knowledge on their production and regulative effects can shed light on essential molecular processes and provide new hints for disease prevention in bivalves. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles PeerJ 4 e1763 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
miRNA biogenesis Mytilus galloprovincialis Crassostrea gigas Bivalves RNAi Medicine R |
spellingShingle |
miRNA biogenesis Mytilus galloprovincialis Crassostrea gigas Bivalves RNAi Medicine R Umberto Rosani Alberto Pallavicini Paola Venier The miRNA biogenesis in marine bivalves |
topic_facet |
miRNA biogenesis Mytilus galloprovincialis Crassostrea gigas Bivalves RNAi Medicine R |
description |
Small non-coding RNAs include powerful regulators of gene expression, transposon mobility and virus activity. Among the various categories, mature microRNAs (miRNAs) guide the translational repression and decay of several targeted mRNAs. The biogenesis of miRNAs depends on few gene products, essentially conserved from basal to higher metazoans, whose protein domains allow specific interactions with dsRNA. Here, we report the identification of key genes responsible of the miRNA biogenesis in 32 bivalves, with particular attention to the aquaculture species Mytilus galloprovincialis and Crassostrea gigas. In detail, we have identified and phylogenetically compared eight evolutionary conserved proteins: DROSHA, DGCR8, EXP5, RAN, DICER TARBP2, AGO and PIWI. In mussels, we recognized several other proteins participating in the miRNA biogenesis or in the subsequent RNA silencing. According to digital expression analysis, these genes display low and not inducible expression levels in adult mussels and oysters whereas they are considerably expressed during development. As miRNAs play an important role also in the antiviral responses, knowledge on their production and regulative effects can shed light on essential molecular processes and provide new hints for disease prevention in bivalves. |
format |
Article in Journal/Newspaper |
author |
Umberto Rosani Alberto Pallavicini Paola Venier |
author_facet |
Umberto Rosani Alberto Pallavicini Paola Venier |
author_sort |
Umberto Rosani |
title |
The miRNA biogenesis in marine bivalves |
title_short |
The miRNA biogenesis in marine bivalves |
title_full |
The miRNA biogenesis in marine bivalves |
title_fullStr |
The miRNA biogenesis in marine bivalves |
title_full_unstemmed |
The miRNA biogenesis in marine bivalves |
title_sort |
mirna biogenesis in marine bivalves |
publisher |
PeerJ Inc. |
publishDate |
2016 |
url |
https://doi.org/10.7717/peerj.1763 https://doaj.org/article/24594bec0567426da90575bb34d95bc6 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
PeerJ, Vol 4, p e1763 (2016) |
op_relation |
https://peerj.com/articles/1763.pdf https://peerj.com/articles/1763/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.1763 2167-8359 https://doaj.org/article/24594bec0567426da90575bb34d95bc6 |
op_doi |
https://doi.org/10.7717/peerj.1763 |
container_title |
PeerJ |
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4 |
container_start_page |
e1763 |
_version_ |
1778524051064487936 |