Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines
Abstract INTRODUCTION: Insect cell cultures play an essential role in understanding arboviral replication. However, the replicative efficiency of some of these viruses such as dengue (DENV), yellow fever (YFV), and chikungunya (CHIKV) in a new cellular substrate (Lulo) and in the other two recognize...
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ftdoajarticles:oai:doaj.org/article:23b8ccc5dda440c4b0be392983a74fcd 2023-05-15T15:08:40+02:00 Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines Nidya Alexandra Segura Guerrero Felio Jesús Bello García 2019-04-01T00:00:00Z https://doi.org/10.1590/0037-8682-0511-2018 https://doaj.org/article/23b8ccc5dda440c4b0be392983a74fcd EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100318&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0511-2018 https://doaj.org/article/23b8ccc5dda440c4b0be392983a74fcd Revista da Sociedade Brasileira de Medicina Tropical, Vol 52, Iss 0 (2019) Flavivirus Alphavirus C6/36 Vero Lulo Replication efficiency Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.1590/0037-8682-0511-2018 2022-12-30T23:24:21Z Abstract INTRODUCTION: Insect cell cultures play an essential role in understanding arboviral replication. However, the replicative efficiency of some of these viruses such as dengue (DENV), yellow fever (YFV), and chikungunya (CHIKV) in a new cellular substrate (Lulo) and in the other two recognized cell lines has not been comparatively assessed. METHODS: Vero, C6/36, and Lulo cell lines were infected with DENV, YFV, and CHIKV. The viral progeny was quantified through plaque assays and quantitative reverse transcription-polymerase chain reaction, while for DENV2, the findings were confirmed by immunofluorescence antibody assay. RESULTS: The higher DENV2 titer (from multiplicity of infection 0.001) was obtained on day four post-infection in C6/36 and on day six in Vero cells, while the Lulo cell line was almost impossible to infect under the same conditions. However, C6/36 showed the highest values of viral RNA production compared to Vero cells, while the quantification of the viral RNA in Lulo cells showed high levels of viral genomes, which had no correlation to the infectious viral particles. CONCLUSIONS: C6/36 was the most efficient cell line in the alpha and flavivirus production, followed by Vero cells. Thus, Lulo cells may be a useful substrate to study the mechanisms by which cells evade viral replication. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 52 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Flavivirus Alphavirus C6/36 Vero Lulo Replication efficiency Arctic medicine. Tropical medicine RC955-962 |
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Flavivirus Alphavirus C6/36 Vero Lulo Replication efficiency Arctic medicine. Tropical medicine RC955-962 Nidya Alexandra Segura Guerrero Felio Jesús Bello García Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines |
topic_facet |
Flavivirus Alphavirus C6/36 Vero Lulo Replication efficiency Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: Insect cell cultures play an essential role in understanding arboviral replication. However, the replicative efficiency of some of these viruses such as dengue (DENV), yellow fever (YFV), and chikungunya (CHIKV) in a new cellular substrate (Lulo) and in the other two recognized cell lines has not been comparatively assessed. METHODS: Vero, C6/36, and Lulo cell lines were infected with DENV, YFV, and CHIKV. The viral progeny was quantified through plaque assays and quantitative reverse transcription-polymerase chain reaction, while for DENV2, the findings were confirmed by immunofluorescence antibody assay. RESULTS: The higher DENV2 titer (from multiplicity of infection 0.001) was obtained on day four post-infection in C6/36 and on day six in Vero cells, while the Lulo cell line was almost impossible to infect under the same conditions. However, C6/36 showed the highest values of viral RNA production compared to Vero cells, while the quantification of the viral RNA in Lulo cells showed high levels of viral genomes, which had no correlation to the infectious viral particles. CONCLUSIONS: C6/36 was the most efficient cell line in the alpha and flavivirus production, followed by Vero cells. Thus, Lulo cells may be a useful substrate to study the mechanisms by which cells evade viral replication. |
format |
Article in Journal/Newspaper |
author |
Nidya Alexandra Segura Guerrero Felio Jesús Bello García |
author_facet |
Nidya Alexandra Segura Guerrero Felio Jesús Bello García |
author_sort |
Nidya Alexandra Segura Guerrero |
title |
Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines |
title_short |
Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines |
title_full |
Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines |
title_fullStr |
Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines |
title_full_unstemmed |
Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines |
title_sort |
comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2019 |
url |
https://doi.org/10.1590/0037-8682-0511-2018 https://doaj.org/article/23b8ccc5dda440c4b0be392983a74fcd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 52, Iss 0 (2019) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100318&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0511-2018 https://doaj.org/article/23b8ccc5dda440c4b0be392983a74fcd |
op_doi |
https://doi.org/10.1590/0037-8682-0511-2018 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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52 |
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