Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean
The V3–V4 hypervariable regions of the 16S ribosomal RNA gene were analyzed to assess prokaryotic diversity and community compositions within 19 surface sediment samples collected from three different regions (depth: 250–3,548 m) of Prydz Bay, the Antarctic Peninsula region, and the Ross Sea. In our...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2020.00783 https://doaj.org/article/23a65ddb5675453aa1343c168f9294da |
id |
ftdoajarticles:oai:doaj.org/article:23a65ddb5675453aa1343c168f9294da |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:23a65ddb5675453aa1343c168f9294da 2023-05-15T13:44:57+02:00 Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean Jiang Li Xiaoqian Gu Yuanyuan Gui 2020-04-01T00:00:00Z https://doi.org/10.3389/fmicb.2020.00783 https://doaj.org/article/23a65ddb5675453aa1343c168f9294da EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2020.00783/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00783 https://doaj.org/article/23a65ddb5675453aa1343c168f9294da Frontiers in Microbiology, Vol 11 (2020) prokaryotic diversity 16S rRNA gene geophysicochemical factors high-throughput sequencing (HTS) the Southern Ocean Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3389/fmicb.2020.00783 2022-12-31T11:40:31Z The V3–V4 hypervariable regions of the 16S ribosomal RNA gene were analyzed to assess prokaryotic diversity and community compositions within 19 surface sediment samples collected from three different regions (depth: 250–3,548 m) of Prydz Bay, the Antarctic Peninsula region, and the Ross Sea. In our results, we characterized 1,079,709 clean tag sequences representing 43,227 operational taxonomic units (OTUs, 97% similarity). The prokaryotic community distribution exhibited obvious geographical differences, and the sequences formed three distinct clusters according to the samples’ origins. In general, the biodiversity of Prydz Bay was higher than those of the Antarctic Peninsula region and the Ross Sea, and there were similar prokaryotic communities in different geographic locations. The most dominant clades in the prokaryotic communities were Proteobacteria, Bacteroidetes, Thaumarchaeota, Oxyphotobacteria, Deinococcus-Thermus, Firmicutes, Acidobacteria, Fusobacteria, and Planctomycetes, but unique prokaryotic community compositions were found in each of the sampling regions. Our results also demonstrated that the prokaryotic diversity and community distribution were mainly influenced by geographical and physicochemical factors, such as Zn, V, Na, K, water depth, and especially geographical distance (longitude variation of sample location) and Ba ion content. Moreover, geochemical factors such as nutrient contents (TC, P, and Ca) also played important roles in prokaryotic diversity and community distribution. This represents the first report that Ba ion content has an obvious effect on prokaryotic diversity and community distribution in Southern Ocean sediments. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Prydz Bay Ross Sea Southern Ocean Directory of Open Access Journals: DOAJ Articles Antarctic Southern Ocean The Antarctic Antarctic Peninsula Ross Sea Prydz Bay Frontiers in Microbiology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
prokaryotic diversity 16S rRNA gene geophysicochemical factors high-throughput sequencing (HTS) the Southern Ocean Microbiology QR1-502 |
spellingShingle |
prokaryotic diversity 16S rRNA gene geophysicochemical factors high-throughput sequencing (HTS) the Southern Ocean Microbiology QR1-502 Jiang Li Xiaoqian Gu Yuanyuan Gui Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean |
topic_facet |
prokaryotic diversity 16S rRNA gene geophysicochemical factors high-throughput sequencing (HTS) the Southern Ocean Microbiology QR1-502 |
description |
The V3–V4 hypervariable regions of the 16S ribosomal RNA gene were analyzed to assess prokaryotic diversity and community compositions within 19 surface sediment samples collected from three different regions (depth: 250–3,548 m) of Prydz Bay, the Antarctic Peninsula region, and the Ross Sea. In our results, we characterized 1,079,709 clean tag sequences representing 43,227 operational taxonomic units (OTUs, 97% similarity). The prokaryotic community distribution exhibited obvious geographical differences, and the sequences formed three distinct clusters according to the samples’ origins. In general, the biodiversity of Prydz Bay was higher than those of the Antarctic Peninsula region and the Ross Sea, and there were similar prokaryotic communities in different geographic locations. The most dominant clades in the prokaryotic communities were Proteobacteria, Bacteroidetes, Thaumarchaeota, Oxyphotobacteria, Deinococcus-Thermus, Firmicutes, Acidobacteria, Fusobacteria, and Planctomycetes, but unique prokaryotic community compositions were found in each of the sampling regions. Our results also demonstrated that the prokaryotic diversity and community distribution were mainly influenced by geographical and physicochemical factors, such as Zn, V, Na, K, water depth, and especially geographical distance (longitude variation of sample location) and Ba ion content. Moreover, geochemical factors such as nutrient contents (TC, P, and Ca) also played important roles in prokaryotic diversity and community distribution. This represents the first report that Ba ion content has an obvious effect on prokaryotic diversity and community distribution in Southern Ocean sediments. |
format |
Article in Journal/Newspaper |
author |
Jiang Li Xiaoqian Gu Yuanyuan Gui |
author_facet |
Jiang Li Xiaoqian Gu Yuanyuan Gui |
author_sort |
Jiang Li |
title |
Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean |
title_short |
Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean |
title_full |
Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean |
title_fullStr |
Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean |
title_full_unstemmed |
Prokaryotic Diversity and Composition of Sediments From Prydz Bay, the Antarctic Peninsula Region, and the Ross Sea, Southern Ocean |
title_sort |
prokaryotic diversity and composition of sediments from prydz bay, the antarctic peninsula region, and the ross sea, southern ocean |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.00783 https://doaj.org/article/23a65ddb5675453aa1343c168f9294da |
geographic |
Antarctic Southern Ocean The Antarctic Antarctic Peninsula Ross Sea Prydz Bay |
geographic_facet |
Antarctic Southern Ocean The Antarctic Antarctic Peninsula Ross Sea Prydz Bay |
genre |
Antarc* Antarctic Antarctic Peninsula Prydz Bay Ross Sea Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Prydz Bay Ross Sea Southern Ocean |
op_source |
Frontiers in Microbiology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00783/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00783 https://doaj.org/article/23a65ddb5675453aa1343c168f9294da |
op_doi |
https://doi.org/10.3389/fmicb.2020.00783 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
_version_ |
1766208891355398144 |