: acquired resistance in mice by implantation of young irradiated worms into the portal system

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Paulo Marcos Z. Coelho, Rómulo Teixeira de Mello, Carlos Alberto P. Tavares
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1989
• Subjects: Schistosoma mansoni; - irradiated worms; immunoprotection; transplantation of worms; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic; The Portal
• Online Access: https://doi.org/10.1590/S0036-46651989000100003; https://doaj.org/article/2399b46e32ea4051a90e4ad9cfd5e357

In two distinct experiments, immature S. mansoni worms (LE strain, Belo Horizonte, Brazil), aged 20 days, obtained from the portal system of white outbred mice, were irradiated with 14 and 4 Krad, respectively. Afterwards, the worms were directly inoculated into the portal vein of normal mice. Inoculation was performed with 20 irradiated worms per animal. Fifty days after inoculation, the mice that received 4 and 14 Krad-irradiated worms and their respective controls were infected with S. mansoni cercariae (LE strain), by transcutaneous route. Twenty days after this challenge infection, the animals were sacrificed and perfused for mature irradiated (90-day-old) and immature (20-day-old) worm counts. Analysis of the results showed that statistically significant protection against cercariae occurred in both groups with irradiated worms.