Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems
Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human inf...
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ftdoajarticles:oai:doaj.org/article:239307427a924fc18a28b22c18583ea9 2023-05-15T14:00:51+02:00 Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio 2020-08-01T00:00:00Z https://doi.org/10.3390/microorganisms8091293 https://doaj.org/article/239307427a924fc18a28b22c18583ea9 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/9/1293 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8091293 2076-2607 https://doaj.org/article/239307427a924fc18a28b22c18583ea9 Microorganisms, Vol 8, Iss 1293, p 1293 (2020) bacteriophages shellfish fish transduction ARG horizontal gene transfer Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8091293 2022-12-31T14:06:08Z Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human influence. Bacteriophages are known to play a relevant role in the dissemination of antibiotic resistance genes (ARGs) in aquatic environments. In this study, five ARGs ( bla TEM , bla CTX-M-1 , bla CTX-M-9 , sul1 and tetW ) were monitored in phage particles isolated from seawater of two different locations: (i) the Mediterranean coast, subjected to high anthropogenic pressure, and (ii) the Antarctic coast, where the anthropogenic impact is low. Although found in lower quantities, ARG-containing phage particles were more prevalent among the Antarctic than the Mediterranean seawater samples and Antarctic bacterial communities were confirmed as their source. In the Mediterranean area, ARG-containing phages from anthropogenic fecal pollution might allow ARG transmission through the food chain. ARGs were detected in phage particles isolated from fish (Mediterranean, Atlantic, farmed, and frozen), the most abundant being β-lactamases. Some of these particles were infectious in cultures of the fecal bacteria Escherichia coli . By serving as ARG reservoirs in marine environments, including those with low human activity, such as the Antarctic, phages could contribute to ARG transmission between bacterial communities. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Microorganisms 8 9 1293 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
bacteriophages shellfish fish transduction ARG horizontal gene transfer Biology (General) QH301-705.5 |
spellingShingle |
bacteriophages shellfish fish transduction ARG horizontal gene transfer Biology (General) QH301-705.5 Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
topic_facet |
bacteriophages shellfish fish transduction ARG horizontal gene transfer Biology (General) QH301-705.5 |
description |
Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human influence. Bacteriophages are known to play a relevant role in the dissemination of antibiotic resistance genes (ARGs) in aquatic environments. In this study, five ARGs ( bla TEM , bla CTX-M-1 , bla CTX-M-9 , sul1 and tetW ) were monitored in phage particles isolated from seawater of two different locations: (i) the Mediterranean coast, subjected to high anthropogenic pressure, and (ii) the Antarctic coast, where the anthropogenic impact is low. Although found in lower quantities, ARG-containing phage particles were more prevalent among the Antarctic than the Mediterranean seawater samples and Antarctic bacterial communities were confirmed as their source. In the Mediterranean area, ARG-containing phages from anthropogenic fecal pollution might allow ARG transmission through the food chain. ARGs were detected in phage particles isolated from fish (Mediterranean, Atlantic, farmed, and frozen), the most abundant being β-lactamases. Some of these particles were infectious in cultures of the fecal bacteria Escherichia coli . By serving as ARG reservoirs in marine environments, including those with low human activity, such as the Antarctic, phages could contribute to ARG transmission between bacterial communities. |
format |
Article in Journal/Newspaper |
author |
Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio |
author_facet |
Pedro Blanco-Picazo Gabriel Roscales Daniel Toribio-Avedillo Clara Gómez-Gómez Conxita Avila Elisenda Ballesté Maite Muniesa Lorena Rodríguez-Rubio |
author_sort |
Pedro Blanco-Picazo |
title |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_short |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_full |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_fullStr |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_full_unstemmed |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_sort |
antibiotic resistance genes in phage particles from antarctic and mediterranean seawater ecosystems |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8091293 https://doaj.org/article/239307427a924fc18a28b22c18583ea9 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Microorganisms, Vol 8, Iss 1293, p 1293 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/9/1293 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8091293 2076-2607 https://doaj.org/article/239307427a924fc18a28b22c18583ea9 |
op_doi |
https://doi.org/10.3390/microorganisms8091293 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
9 |
container_start_page |
1293 |
_version_ |
1766270185689317376 |