Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs.
Endemic in Brazil, visceral leishmaniasis (VL) is a zoonotic infection that is among the most important parasitic diseases transmitted by vectors. Dogs are the main reservoirs of canine leishmaniasis (CanL) and their identification is used in some countries as part of disease prevention and control...
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Online Access: | https://doi.org/10.1371/journal.pntd.0011535 https://doaj.org/article/237fec29dca246e9ae5179cc113a9b93 |
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ftdoajarticles:oai:doaj.org/article:237fec29dca246e9ae5179cc113a9b93 2023-11-05T03:40:06+01:00 Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs. Williane Fernanda Siqueira Mariana Santos Cardoso Vanessa Gomes Fraga Jennifer Ottino Vitor Márcio Ribeiro Carolina Novato Gondim Joziana Muniz de Paiva Barçante Ana Carolina Amado Gomes Alexsandro Sobreira Galdino Kasper Eersels Bart van Grinsven Daniella Castanheira Bartholomeu Lilian Lacerda Bueno Thomas Cleij Ricardo Toshio Fujiwara 2023-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011535 https://doaj.org/article/237fec29dca246e9ae5179cc113a9b93 EN eng Public Library of Science (PLoS) https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011535&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011535 https://doaj.org/article/237fec29dca246e9ae5179cc113a9b93 PLoS Neglected Tropical Diseases, Vol 17, Iss 8, p e0011535 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011535 2023-10-08T00:37:57Z Endemic in Brazil, visceral leishmaniasis (VL) is a zoonotic infection that is among the most important parasitic diseases transmitted by vectors. Dogs are the main reservoirs of canine leishmaniasis (CanL) and their identification is used in some countries as part of disease prevention and control measures in the canine and human population. In this context, serological tests are necessary, composed of antigens capable of correctly identifying infected dogs, minimizing the number of false-negative cases. This study aimed to identify more immunoreactive peptides derived from two previously described whole proteins (rDyn-1 and rKDDR-plus) and compare their performance to the control antigens rK39 and the crude extract for the detection of dogs infected with L. infantum, especially the asymptomatic ones. The three selected peptides and a mixture of them, along with the rDyn-1, rKDDR-plus, rK39, and crude extract antigens were evaluated using indirect ELISA with sera samples from 186 dogs with CanL, being asymptomatic (n = 50), symptomatic (n = 50), co-infected (n = 19), infected with Babesia sp. (n = 7), Ehrlichia sp. (n = 6), T. cruzi (n = 20) and uninfected (n = 34). The results showed that the rDyn-1 protein and the peptide mixture had the highest sensitivity (100% and 98.32%, respectively) and specificity (97.01 and 98.51, respectively). A high degree of kappa agreement was found for rDyn-1 protein (0.977), mixed peptides (0.965), rKDDR-plus protein (0.953), K-plus peptide 1 (0.930) and Dyn-1 peptide (0.893). The mixture of peptides showed the highest likelihood (65.87). The ELISA using the mixture of peptides and the rDyn-1 protein showed high performance for CanL serodiagnosis. More mix combinations of the peptides and additional extended field tests with a larger sample size are recommended. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles PLOS Neglected Tropical Diseases 17 8 e0011535 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Williane Fernanda Siqueira Mariana Santos Cardoso Vanessa Gomes Fraga Jennifer Ottino Vitor Márcio Ribeiro Carolina Novato Gondim Joziana Muniz de Paiva Barçante Ana Carolina Amado Gomes Alexsandro Sobreira Galdino Kasper Eersels Bart van Grinsven Daniella Castanheira Bartholomeu Lilian Lacerda Bueno Thomas Cleij Ricardo Toshio Fujiwara Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Endemic in Brazil, visceral leishmaniasis (VL) is a zoonotic infection that is among the most important parasitic diseases transmitted by vectors. Dogs are the main reservoirs of canine leishmaniasis (CanL) and their identification is used in some countries as part of disease prevention and control measures in the canine and human population. In this context, serological tests are necessary, composed of antigens capable of correctly identifying infected dogs, minimizing the number of false-negative cases. This study aimed to identify more immunoreactive peptides derived from two previously described whole proteins (rDyn-1 and rKDDR-plus) and compare their performance to the control antigens rK39 and the crude extract for the detection of dogs infected with L. infantum, especially the asymptomatic ones. The three selected peptides and a mixture of them, along with the rDyn-1, rKDDR-plus, rK39, and crude extract antigens were evaluated using indirect ELISA with sera samples from 186 dogs with CanL, being asymptomatic (n = 50), symptomatic (n = 50), co-infected (n = 19), infected with Babesia sp. (n = 7), Ehrlichia sp. (n = 6), T. cruzi (n = 20) and uninfected (n = 34). The results showed that the rDyn-1 protein and the peptide mixture had the highest sensitivity (100% and 98.32%, respectively) and specificity (97.01 and 98.51, respectively). A high degree of kappa agreement was found for rDyn-1 protein (0.977), mixed peptides (0.965), rKDDR-plus protein (0.953), K-plus peptide 1 (0.930) and Dyn-1 peptide (0.893). The mixture of peptides showed the highest likelihood (65.87). The ELISA using the mixture of peptides and the rDyn-1 protein showed high performance for CanL serodiagnosis. More mix combinations of the peptides and additional extended field tests with a larger sample size are recommended. |
format |
Article in Journal/Newspaper |
author |
Williane Fernanda Siqueira Mariana Santos Cardoso Vanessa Gomes Fraga Jennifer Ottino Vitor Márcio Ribeiro Carolina Novato Gondim Joziana Muniz de Paiva Barçante Ana Carolina Amado Gomes Alexsandro Sobreira Galdino Kasper Eersels Bart van Grinsven Daniella Castanheira Bartholomeu Lilian Lacerda Bueno Thomas Cleij Ricardo Toshio Fujiwara |
author_facet |
Williane Fernanda Siqueira Mariana Santos Cardoso Vanessa Gomes Fraga Jennifer Ottino Vitor Márcio Ribeiro Carolina Novato Gondim Joziana Muniz de Paiva Barçante Ana Carolina Amado Gomes Alexsandro Sobreira Galdino Kasper Eersels Bart van Grinsven Daniella Castanheira Bartholomeu Lilian Lacerda Bueno Thomas Cleij Ricardo Toshio Fujiwara |
author_sort |
Williane Fernanda Siqueira |
title |
Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs. |
title_short |
Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs. |
title_full |
Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs. |
title_fullStr |
Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs. |
title_full_unstemmed |
Immunogenic mapping of rDyn-1 and rKDDR-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of Leishmania infantum-infected dogs. |
title_sort |
immunogenic mapping of rdyn-1 and rkddr-plus proteins and selection of oligopeptides by immunoblotting for the diagnosis of leishmania infantum-infected dogs. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011535 https://doaj.org/article/237fec29dca246e9ae5179cc113a9b93 |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 8, p e0011535 (2023) |
op_relation |
https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011535&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011535 https://doaj.org/article/237fec29dca246e9ae5179cc113a9b93 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011535 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
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8 |
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e0011535 |
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