Opportunities for improving pLDH-based malaria diagnostic tests
Abstract Background Monoclonal antibodies to Plasmodium lactate dehydrogenase (pLDH) have been previously used to format immunochromatographic tests for the diagnosis of malaria. Using pLDH as an antigen has several advantages as a sensitive measure of the presence of parasites within patient blood...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-10-213 https://doaj.org/article/236d00798f9b45ca9ec0c85686026fe3 |
id |
ftdoajarticles:oai:doaj.org/article:236d00798f9b45ca9ec0c85686026fe3 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:236d00798f9b45ca9ec0c85686026fe3 2023-05-15T15:10:49+02:00 Opportunities for improving pLDH-based malaria diagnostic tests Choi Young Buchanan Ian Piper Robert C Makler Michael T 2011-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-213 https://doaj.org/article/236d00798f9b45ca9ec0c85686026fe3 EN eng BMC http://www.malariajournal.com/content/10/1/213 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-213 1475-2875 https://doaj.org/article/236d00798f9b45ca9ec0c85686026fe3 Malaria Journal, Vol 10, Iss 1, p 213 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-213 2022-12-31T00:34:34Z Abstract Background Monoclonal antibodies to Plasmodium lactate dehydrogenase (pLDH) have been previously used to format immunochromatographic tests for the diagnosis of malaria. Using pLDH as an antigen has several advantages as a sensitive measure of the presence of parasites within patient blood samples. However, variable results in terms of specificity and sensitivity among different commercially available diagnostic kits have been reported and it has not been clear from these studies whether the performance of an individual test is due simply to how it is engineered or whether it is due to the biochemical nature of the pLDH-antibody reaction itself. Methods A series of systematic studies to determine how various pLDH monoclonal antibodies work in combination was undertaken. Different combinations of anti-pLDH monoclonal antibodies were used in a rapid-test immunochromatographic assay format to determine parameters of sensitivity and specificity with regard to individual Plasmodium species. Results Dramatic differences were found in both species specificity and overall sensitivity depending on which antibody is used on the immunochromatographic strip and which is used on the colorimetric colloidal-gold used for visual detection. Discussion The results demonstrate the feasibility of different test formats for the detection and speciation of malarial infections. In addition, the data will enable the development of a universal rapid test algorithm that may potentially provide a cost-effective strategy to diagnose and manage patients in a wide range of clinical settings. Conclusion These data emphasize that using different anti-pLDH antibody combinations offers a tractable way to optimize immunochromatographic pLDH tests. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Choi Young Buchanan Ian Piper Robert C Makler Michael T Opportunities for improving pLDH-based malaria diagnostic tests |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Monoclonal antibodies to Plasmodium lactate dehydrogenase (pLDH) have been previously used to format immunochromatographic tests for the diagnosis of malaria. Using pLDH as an antigen has several advantages as a sensitive measure of the presence of parasites within patient blood samples. However, variable results in terms of specificity and sensitivity among different commercially available diagnostic kits have been reported and it has not been clear from these studies whether the performance of an individual test is due simply to how it is engineered or whether it is due to the biochemical nature of the pLDH-antibody reaction itself. Methods A series of systematic studies to determine how various pLDH monoclonal antibodies work in combination was undertaken. Different combinations of anti-pLDH monoclonal antibodies were used in a rapid-test immunochromatographic assay format to determine parameters of sensitivity and specificity with regard to individual Plasmodium species. Results Dramatic differences were found in both species specificity and overall sensitivity depending on which antibody is used on the immunochromatographic strip and which is used on the colorimetric colloidal-gold used for visual detection. Discussion The results demonstrate the feasibility of different test formats for the detection and speciation of malarial infections. In addition, the data will enable the development of a universal rapid test algorithm that may potentially provide a cost-effective strategy to diagnose and manage patients in a wide range of clinical settings. Conclusion These data emphasize that using different anti-pLDH antibody combinations offers a tractable way to optimize immunochromatographic pLDH tests. |
format |
Article in Journal/Newspaper |
author |
Choi Young Buchanan Ian Piper Robert C Makler Michael T |
author_facet |
Choi Young Buchanan Ian Piper Robert C Makler Michael T |
author_sort |
Choi Young |
title |
Opportunities for improving pLDH-based malaria diagnostic tests |
title_short |
Opportunities for improving pLDH-based malaria diagnostic tests |
title_full |
Opportunities for improving pLDH-based malaria diagnostic tests |
title_fullStr |
Opportunities for improving pLDH-based malaria diagnostic tests |
title_full_unstemmed |
Opportunities for improving pLDH-based malaria diagnostic tests |
title_sort |
opportunities for improving pldh-based malaria diagnostic tests |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-213 https://doaj.org/article/236d00798f9b45ca9ec0c85686026fe3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 213 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/213 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-213 1475-2875 https://doaj.org/article/236d00798f9b45ca9ec0c85686026fe3 |
op_doi |
https://doi.org/10.1186/1475-2875-10-213 |
container_title |
Malaria Journal |
container_volume |
10 |
container_issue |
1 |
_version_ |
1766341771852251136 |