Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA
Protocols used to collect fish skin mucus may inadvertently compromise the sampled fish or the resulting sample. Here, we used three methods (wiping, scraping, and absorption) to collect skin mucus from Atlantic salmon and compared their invasiveness on fish skin epithelium. We found that the absorp...
Published in: | Animals |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/ani10081374 https://doaj.org/article/2369101bb1944120814917ffd5083248 |
id |
ftdoajarticles:oai:doaj.org/article:2369101bb1944120814917ffd5083248 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:2369101bb1944120814917ffd5083248 2023-05-15T15:30:45+02:00 Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA Haitham Tartor Adérito Luis Monjane Søren Grove 2020-08-01T00:00:00Z https://doi.org/10.3390/ani10081374 https://doaj.org/article/2369101bb1944120814917ffd5083248 EN eng MDPI AG https://www.mdpi.com/2076-2615/10/8/1374 https://doaj.org/toc/2076-2615 doi:10.3390/ani10081374 2076-2615 https://doaj.org/article/2369101bb1944120814917ffd5083248 Animals, Vol 10, Iss 1374, p 1374 (2020) Atlantic salmon skin mucus gill mucus minimally invasive sampling specific IgM antibodies complement component 5 Veterinary medicine SF600-1100 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.3390/ani10081374 2022-12-31T13:18:27Z Protocols used to collect fish skin mucus may inadvertently compromise the sampled fish or the resulting sample. Here, we used three methods (wiping, scraping, and absorption) to collect skin mucus from Atlantic salmon and compared their invasiveness on fish skin epithelium. We found that the absorption method was the least invasive. We also compared the abundance of antigen-specific immunoglobulin M subtype A antibodies (IgM-A Ab) and complement component 5 (C5) in mucus samples collected from vaccinated fish by the three methods. An enzyme-cascade-amplification strategy colorimetric immune assay was optimized and used to analyze IgM-A, and ELISA was used to analyze C5. The abundance of antigen-specific IgM-A in skin mucus was comparable between the three methods, but C5 was significantly lower in absorbed mucus in comparison to in the wiped or scraped mucus samples. Absorbed skin mucus samples collected from various body regions of salmon, levels of C5 were comparable, while specific IgM-A amounts varied between the regions. By comparing three mucus-absorbing materials (medical wipe, gauze, and cotton) for their ability to absorb and release IgM-A and C5, medical wipes proved to be ideal for IgM-A analysis, whereas gauze was the best for C5 analysis. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Animals 10 8 1374 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon skin mucus gill mucus minimally invasive sampling specific IgM antibodies complement component 5 Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
Atlantic salmon skin mucus gill mucus minimally invasive sampling specific IgM antibodies complement component 5 Veterinary medicine SF600-1100 Zoology QL1-991 Haitham Tartor Adérito Luis Monjane Søren Grove Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA |
topic_facet |
Atlantic salmon skin mucus gill mucus minimally invasive sampling specific IgM antibodies complement component 5 Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
Protocols used to collect fish skin mucus may inadvertently compromise the sampled fish or the resulting sample. Here, we used three methods (wiping, scraping, and absorption) to collect skin mucus from Atlantic salmon and compared their invasiveness on fish skin epithelium. We found that the absorption method was the least invasive. We also compared the abundance of antigen-specific immunoglobulin M subtype A antibodies (IgM-A Ab) and complement component 5 (C5) in mucus samples collected from vaccinated fish by the three methods. An enzyme-cascade-amplification strategy colorimetric immune assay was optimized and used to analyze IgM-A, and ELISA was used to analyze C5. The abundance of antigen-specific IgM-A in skin mucus was comparable between the three methods, but C5 was significantly lower in absorbed mucus in comparison to in the wiped or scraped mucus samples. Absorbed skin mucus samples collected from various body regions of salmon, levels of C5 were comparable, while specific IgM-A amounts varied between the regions. By comparing three mucus-absorbing materials (medical wipe, gauze, and cotton) for their ability to absorb and release IgM-A and C5, medical wipes proved to be ideal for IgM-A analysis, whereas gauze was the best for C5 analysis. |
format |
Article in Journal/Newspaper |
author |
Haitham Tartor Adérito Luis Monjane Søren Grove |
author_facet |
Haitham Tartor Adérito Luis Monjane Søren Grove |
author_sort |
Haitham Tartor |
title |
Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA |
title_short |
Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA |
title_full |
Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA |
title_fullStr |
Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA |
title_full_unstemmed |
Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA |
title_sort |
quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ani10081374 https://doaj.org/article/2369101bb1944120814917ffd5083248 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Animals, Vol 10, Iss 1374, p 1374 (2020) |
op_relation |
https://www.mdpi.com/2076-2615/10/8/1374 https://doaj.org/toc/2076-2615 doi:10.3390/ani10081374 2076-2615 https://doaj.org/article/2369101bb1944120814917ffd5083248 |
op_doi |
https://doi.org/10.3390/ani10081374 |
container_title |
Animals |
container_volume |
10 |
container_issue |
8 |
container_start_page |
1374 |
_version_ |
1766361204683440128 |