Molecular analysis of the cold tolerant Antarctic nematode, Panagrolaimus davidi.

• Published in: PLoS ONE
• Main Authors: Michael A S Thorne, Hiroshi Kagoshima, Melody S Clark, Craig J Marshall, David A Wharton
• Format: Article in Journal/Newspaper
• Language: English
• Published: Public Library of Science (PLoS) 2014
• Subjects: Medicine; R; Science; Q; Antarctic; The Antarctic; Antarc*
• Online Access: https://doi.org/10.1371/journal.pone.0104526; https://doaj.org/article/2345e1b0aa89416db64997b1adee46bb

Isolated and established in culture from the Antarctic in 1988, the nematode Panagrolaimus davidi has proven to be an ideal model for the study of adaptation to the cold. Not only is it the best-documented example of an organism surviving intracellular freezing but it is also able to undergo cryoprotective dehydration. As part of an ongoing effort to develop a molecular understanding of this remarkable organism, we have assembled both a transcriptome and a set of genomic scaffolds. We provide an overview of the transcriptome and a survey of genes involved in temperature stress. We also explore, in silico, the possibility that P. davidi will be susceptible to an environmental RNAi response, important for further functional studies.