Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya.
The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical...
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ftdoajarticles:oai:doaj.org/article:22e7993f6e924f1dab41dfd259e1624c 2023-05-15T15:08:46+02:00 Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya. Iman B Hassaballa Catherine L Sole Xavier Cheseto Baldwyn Torto David P Tchouassi 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009041 https://doaj.org/article/22e7993f6e924f1dab41dfd259e1624c EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009041 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009041 https://doaj.org/article/22e7993f6e924f1dab41dfd259e1624c PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009041 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009041 2022-12-31T09:15:14Z The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical approaches, we decipher specific plant-feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya. Cold-anthrone test indicative of recent plant feeding showed that fructose positivity rates were similar in both sand fly sexes and between those sampled indoors and outdoors. Analysis of derived sequences of the ribulose-1,5-bisphosphate carboxylase large subunit gene (rbcL) from fructose-positive specimens implicated mainly Acacia plants in the family Fabaceae (73%) as those readily foraged on by both sexes of Phlebotomus and Sergentomyia. Chemical analysis by high performance liquid chromatography detected fructose as the most common sugar in sand flies and leaves of selected plant species in the Fabaceae family. Analysis of similarities (ANOSIM) of the headspace volatile profiles of selected Fabaceae plants identified benzyl alcohol, (Z)-linalool oxide, (E)-β-ocimene, p-cymene, p-cresol, and m-cresol, as discriminating compounds between the plant volatiles. These results indicate selective sand fly plant feeding and suggest that the discriminating volatile organic compounds could be exploited in attractive toxic sugar- and odor- bait technologies control strategies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 2 e0009041 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Iman B Hassaballa Catherine L Sole Xavier Cheseto Baldwyn Torto David P Tchouassi Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical approaches, we decipher specific plant-feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya. Cold-anthrone test indicative of recent plant feeding showed that fructose positivity rates were similar in both sand fly sexes and between those sampled indoors and outdoors. Analysis of derived sequences of the ribulose-1,5-bisphosphate carboxylase large subunit gene (rbcL) from fructose-positive specimens implicated mainly Acacia plants in the family Fabaceae (73%) as those readily foraged on by both sexes of Phlebotomus and Sergentomyia. Chemical analysis by high performance liquid chromatography detected fructose as the most common sugar in sand flies and leaves of selected plant species in the Fabaceae family. Analysis of similarities (ANOSIM) of the headspace volatile profiles of selected Fabaceae plants identified benzyl alcohol, (Z)-linalool oxide, (E)-β-ocimene, p-cymene, p-cresol, and m-cresol, as discriminating compounds between the plant volatiles. These results indicate selective sand fly plant feeding and suggest that the discriminating volatile organic compounds could be exploited in attractive toxic sugar- and odor- bait technologies control strategies. |
format |
Article in Journal/Newspaper |
author |
Iman B Hassaballa Catherine L Sole Xavier Cheseto Baldwyn Torto David P Tchouassi |
author_facet |
Iman B Hassaballa Catherine L Sole Xavier Cheseto Baldwyn Torto David P Tchouassi |
author_sort |
Iman B Hassaballa |
title |
Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya. |
title_short |
Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya. |
title_full |
Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya. |
title_fullStr |
Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya. |
title_full_unstemmed |
Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya. |
title_sort |
afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, kenya. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009041 https://doaj.org/article/22e7993f6e924f1dab41dfd259e1624c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009041 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009041 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009041 https://doaj.org/article/22e7993f6e924f1dab41dfd259e1624c |
op_doi |
https://doi.org/10.1371/journal.pntd.0009041 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
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2 |
container_start_page |
e0009041 |
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1766340064565002240 |