Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening.

• Published in: PLOS ONE
• Main Authors: Daniela Quaglia, Maximilian C C J C Ebert, Paul F Mugford, Joelle N Pelletier
• Format: Article in Journal/Newspaper
• Language: English
• Published: Public Library of Science (PLoS) 2017
• Subjects: Medicine; R; Science; Q; Golden Gate; Antarc*; Antarctica
• Online Access: https://doi.org/10.1371/journal.pone.0171741; https://doaj.org/article/22ccb32669e245bfbd118bd9a69d8e5b

The Golden Gate strategy entails the use of type IIS restriction enzymes, which cut outside of their recognition sequence. It enables unrestricted design of unique DNA fragments that can be readily and seamlessly recombined. Successfully employed in other synthetic biology applications, we demonstra...