Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles
ABSTRACT A study with transmission electron microscopy of mycoplasma-contaminated HeLa cells using five cell donors referred to as donors A, B, C, D and E, observations are herein presented. Experiments performed with cells from donors B, C and D, revealed the presence of Mycoplasma hyorhinis after...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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2017
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Online Access: | https://doi.org/10.1590/s1678-9946201759084 https://doaj.org/article/22bda5f3dd9f40a889c5d6d3b0995bd6 |
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ftdoajarticles:oai:doaj.org/article:22bda5f3dd9f40a889c5d6d3b0995bd6 2024-09-09T19:27:21+00:00 Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles Antonio Sesso Edite Hatsumi Yamashiro-Kanashiro Liã Bárbara Arruda Joyce Kawakami Maria de Lourdes Higuchi Noemia Mie Orii Noemi Nosomi Taniwaki Flávia Mendes da Cunha Carvalho Mariane Pereira Brito Maiara Gottardi Sylvia Mendes Carneiro Rumio Taga 2017-12-01T00:00:00Z https://doi.org/10.1590/s1678-9946201759084 https://doaj.org/article/22bda5f3dd9f40a889c5d6d3b0995bd6 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000251&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759084 https://doaj.org/article/22bda5f3dd9f40a889c5d6d3b0995bd6 Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 (2017) Mycoplasma Endoplasmic reticulum Mitochondria Cytosol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1590/s1678-9946201759084 2024-08-05T17:49:30Z ABSTRACT A study with transmission electron microscopy of mycoplasma-contaminated HeLa cells using five cell donors referred to as donors A, B, C, D and E, observations are herein presented. Experiments performed with cells from donors B, C and D, revealed the presence of Mycoplasma hyorhinis after PCR and sequencing experiments. Bacteria probably originated from a cytoplasm with compacted tiny granular particles replacing the normal cytosol territories, or from the contact with the cytoplasm through a clear semi-solid material. The compact granularity (CG) of the cytoplasm was crossed by stripes of smooth and rough endoplasmic reticulum cisternae. Among apparently normal mitochondria, it was noted, in variable proportions, mitochondria with crista-delimited lucent central regions that expand to and occupied the interior of a crista-less organelle, which can undergo fission. Other components of the scenarios of mycoplasma-induced cell demolition are villus-like structures with associated 80-200 nm vesicles and a clear, flexible semi-solid, process-sensitive substance that we named jam-like material. This material coated the cytoplasmic surface, its recesses, irregular protrusions and detached cytoplasmic fragments. It also cushioned forming bacteria. Cyst-like structures were often present in the cytoplasm. Cells, mainly apoptotic, exhibiting ample cytoplasmic sectors with characteristic net-like profile due to adjoined vacuoles, as well as ovoid or elongated profiles, consistently appeared in all cells from the last four cell donors. These cells were named “modified host cells” because bacteria arose in the vacuoles. The possibility that, in some samples, there was infection and/or coinfection of the host cell by another organism(s) cannot be ruled out. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 59 0 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Mycoplasma Endoplasmic reticulum Mitochondria Cytosol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Mycoplasma Endoplasmic reticulum Mitochondria Cytosol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Antonio Sesso Edite Hatsumi Yamashiro-Kanashiro Liã Bárbara Arruda Joyce Kawakami Maria de Lourdes Higuchi Noemia Mie Orii Noemi Nosomi Taniwaki Flávia Mendes da Cunha Carvalho Mariane Pereira Brito Maiara Gottardi Sylvia Mendes Carneiro Rumio Taga Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles |
topic_facet |
Mycoplasma Endoplasmic reticulum Mitochondria Cytosol Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT A study with transmission electron microscopy of mycoplasma-contaminated HeLa cells using five cell donors referred to as donors A, B, C, D and E, observations are herein presented. Experiments performed with cells from donors B, C and D, revealed the presence of Mycoplasma hyorhinis after PCR and sequencing experiments. Bacteria probably originated from a cytoplasm with compacted tiny granular particles replacing the normal cytosol territories, or from the contact with the cytoplasm through a clear semi-solid material. The compact granularity (CG) of the cytoplasm was crossed by stripes of smooth and rough endoplasmic reticulum cisternae. Among apparently normal mitochondria, it was noted, in variable proportions, mitochondria with crista-delimited lucent central regions that expand to and occupied the interior of a crista-less organelle, which can undergo fission. Other components of the scenarios of mycoplasma-induced cell demolition are villus-like structures with associated 80-200 nm vesicles and a clear, flexible semi-solid, process-sensitive substance that we named jam-like material. This material coated the cytoplasmic surface, its recesses, irregular protrusions and detached cytoplasmic fragments. It also cushioned forming bacteria. Cyst-like structures were often present in the cytoplasm. Cells, mainly apoptotic, exhibiting ample cytoplasmic sectors with characteristic net-like profile due to adjoined vacuoles, as well as ovoid or elongated profiles, consistently appeared in all cells from the last four cell donors. These cells were named “modified host cells” because bacteria arose in the vacuoles. The possibility that, in some samples, there was infection and/or coinfection of the host cell by another organism(s) cannot be ruled out. |
format |
Article in Journal/Newspaper |
author |
Antonio Sesso Edite Hatsumi Yamashiro-Kanashiro Liã Bárbara Arruda Joyce Kawakami Maria de Lourdes Higuchi Noemia Mie Orii Noemi Nosomi Taniwaki Flávia Mendes da Cunha Carvalho Mariane Pereira Brito Maiara Gottardi Sylvia Mendes Carneiro Rumio Taga |
author_facet |
Antonio Sesso Edite Hatsumi Yamashiro-Kanashiro Liã Bárbara Arruda Joyce Kawakami Maria de Lourdes Higuchi Noemia Mie Orii Noemi Nosomi Taniwaki Flávia Mendes da Cunha Carvalho Mariane Pereira Brito Maiara Gottardi Sylvia Mendes Carneiro Rumio Taga |
author_sort |
Antonio Sesso |
title |
Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles |
title_short |
Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles |
title_full |
Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles |
title_fullStr |
Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles |
title_full_unstemmed |
Bacteria arise at the border of mycoplasma-infected HeLa cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles |
title_sort |
bacteria arise at the border of mycoplasma-infected hela cells, containing cytoplasm with either malformed cytosol, mitochondria and endoplasmic reticulum or tightly adjoined smooth vacuoles |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2017 |
url |
https://doi.org/10.1590/s1678-9946201759084 https://doaj.org/article/22bda5f3dd9f40a889c5d6d3b0995bd6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 (2017) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000251&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759084 https://doaj.org/article/22bda5f3dd9f40a889c5d6d3b0995bd6 |
op_doi |
https://doi.org/10.1590/s1678-9946201759084 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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59 |
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