Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA)
In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive proced...
Main Authors: | , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
University of Guilan
2014
|
Subjects: | |
Online Access: | https://doaj.org/article/229ce55c61154654b2f193fa4f772bc3 |
id |
ftdoajarticles:oai:doaj.org/article:229ce55c61154654b2f193fa4f772bc3 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:229ce55c61154654b2f193fa4f772bc3 2023-05-15T15:41:41+02:00 Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. 2014-10-01T00:00:00Z https://doaj.org/article/229ce55c61154654b2f193fa4f772bc3 EN eng University of Guilan https://cjes.guilan.ac.ir/article_142.html https://doaj.org/toc/1735-3033 https://doaj.org/toc/1735-3866 1735-3033 1735-3866 https://doaj.org/article/229ce55c61154654b2f193fa4f772bc3 Caspian Journal of Environmental Sciences, Vol 12, Iss 2, Pp 185-195 (2014) bulked segregant analysis (BSA) genetics sex marker Huso huso Beluga Environmental sciences GE1-350 Science Q article 2014 ftdoajarticles 2022-12-31T07:17:17Z In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species. Article in Journal/Newspaper Beluga Beluga* Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
bulked segregant analysis (BSA) genetics sex marker Huso huso Beluga Environmental sciences GE1-350 Science Q |
spellingShingle |
bulked segregant analysis (BSA) genetics sex marker Huso huso Beluga Environmental sciences GE1-350 Science Q Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
topic_facet |
bulked segregant analysis (BSA) genetics sex marker Huso huso Beluga Environmental sciences GE1-350 Science Q |
description |
In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species. |
format |
Article in Journal/Newspaper |
author |
Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. |
author_facet |
Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. |
author_sort |
Khodaparast, M. |
title |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_short |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_full |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_fullStr |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_full_unstemmed |
Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA) |
title_sort |
searching the genome of beluga (huso huso) for sex markers based on targeted bulked segregant analysis (bsa) |
publisher |
University of Guilan |
publishDate |
2014 |
url |
https://doaj.org/article/229ce55c61154654b2f193fa4f772bc3 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Caspian Journal of Environmental Sciences, Vol 12, Iss 2, Pp 185-195 (2014) |
op_relation |
https://cjes.guilan.ac.ir/article_142.html https://doaj.org/toc/1735-3033 https://doaj.org/toc/1735-3866 1735-3033 1735-3866 https://doaj.org/article/229ce55c61154654b2f193fa4f772bc3 |
_version_ |
1766374573486374912 |