Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse
Higher plants are an integral part of strategies for sustained human presence in space. Space-based greenhouses have the potential to provide closed-loop recycling of oxygen, water and food. Plant monitoring systems with the capacity to remotely observe the condition of crops in real-time within the...
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ftdoajarticles:oai:doaj.org/article:227252fa3ef94c6da3e507f46f201022 2023-05-15T14:59:58+02:00 Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse Alain Berinstain Rita Noumeir Stephen Braham Thomas Graham Anna-Lisa Paul Matthew Bamsey Talal Abboud Robert Ferl 2013-03-01T00:00:00Z https://doi.org/10.3390/s130303530 https://doaj.org/article/227252fa3ef94c6da3e507f46f201022 EN eng MDPI AG http://www.mdpi.com/1424-8220/13/3/3530 https://doaj.org/toc/1424-8220 doi:10.3390/s130303530 1424-8220 https://doaj.org/article/227252fa3ef94c6da3e507f46f201022 Sensors, Vol 13, Iss 3, Pp 3530-3548 (2013) green fluorescent protein remote sensor telemetry plant health life support mars astrobiology analogue environments imaging Chemical technology TP1-1185 article 2013 ftdoajarticles https://doi.org/10.3390/s130303530 2023-01-08T01:26:36Z Higher plants are an integral part of strategies for sustained human presence in space. Space-based greenhouses have the potential to provide closed-loop recycling of oxygen, water and food. Plant monitoring systems with the capacity to remotely observe the condition of crops in real-time within these systems would permit operators to take immediate action to ensure optimum system yield and reliability. One such plant health monitoring technique involves the use of reporter genes driving fluorescent proteins as biological sensors of plant stress. In 2006 an initial prototype green fluorescent protein imager system was deployed at the Arthur Clarke Mars Greenhouse located in the Canadian High Arctic. This prototype demonstrated the advantageous of this biosensor technology and underscored the challenges in collecting and managing telemetric data from exigent environments. We present here the design and deployment of a second prototype imaging system deployed within and connected to the infrastructure of the Arthur Clarke Mars Greenhouse. This is the first imager to run autonomously for one year in the un-crewed greenhouse with command and control conducted through the greenhouse satellite control system. Images were saved locally in high resolution and sent telemetrically in low resolution. Imager hardware is described, including the custom designed LED growth light and fluorescent excitation light boards, filters, data acquisition and control system, and basic sensing and environmental control. Several critical lessons learned related to the hardware of small plant growth payloads are also elaborated. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Sensors 13 3 3530 3548 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
green fluorescent protein remote sensor telemetry plant health life support mars astrobiology analogue environments imaging Chemical technology TP1-1185 |
spellingShingle |
green fluorescent protein remote sensor telemetry plant health life support mars astrobiology analogue environments imaging Chemical technology TP1-1185 Alain Berinstain Rita Noumeir Stephen Braham Thomas Graham Anna-Lisa Paul Matthew Bamsey Talal Abboud Robert Ferl Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
topic_facet |
green fluorescent protein remote sensor telemetry plant health life support mars astrobiology analogue environments imaging Chemical technology TP1-1185 |
description |
Higher plants are an integral part of strategies for sustained human presence in space. Space-based greenhouses have the potential to provide closed-loop recycling of oxygen, water and food. Plant monitoring systems with the capacity to remotely observe the condition of crops in real-time within these systems would permit operators to take immediate action to ensure optimum system yield and reliability. One such plant health monitoring technique involves the use of reporter genes driving fluorescent proteins as biological sensors of plant stress. In 2006 an initial prototype green fluorescent protein imager system was deployed at the Arthur Clarke Mars Greenhouse located in the Canadian High Arctic. This prototype demonstrated the advantageous of this biosensor technology and underscored the challenges in collecting and managing telemetric data from exigent environments. We present here the design and deployment of a second prototype imaging system deployed within and connected to the infrastructure of the Arthur Clarke Mars Greenhouse. This is the first imager to run autonomously for one year in the un-crewed greenhouse with command and control conducted through the greenhouse satellite control system. Images were saved locally in high resolution and sent telemetrically in low resolution. Imager hardware is described, including the custom designed LED growth light and fluorescent excitation light boards, filters, data acquisition and control system, and basic sensing and environmental control. Several critical lessons learned related to the hardware of small plant growth payloads are also elaborated. |
format |
Article in Journal/Newspaper |
author |
Alain Berinstain Rita Noumeir Stephen Braham Thomas Graham Anna-Lisa Paul Matthew Bamsey Talal Abboud Robert Ferl |
author_facet |
Alain Berinstain Rita Noumeir Stephen Braham Thomas Graham Anna-Lisa Paul Matthew Bamsey Talal Abboud Robert Ferl |
author_sort |
Alain Berinstain |
title |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_short |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_full |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_fullStr |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_full_unstemmed |
Deployment of a Fully-Automated Green Fluorescent Protein Imaging System in a High Arctic Autonomous Greenhouse |
title_sort |
deployment of a fully-automated green fluorescent protein imaging system in a high arctic autonomous greenhouse |
publisher |
MDPI AG |
publishDate |
2013 |
url |
https://doi.org/10.3390/s130303530 https://doaj.org/article/227252fa3ef94c6da3e507f46f201022 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Sensors, Vol 13, Iss 3, Pp 3530-3548 (2013) |
op_relation |
http://www.mdpi.com/1424-8220/13/3/3530 https://doaj.org/toc/1424-8220 doi:10.3390/s130303530 1424-8220 https://doaj.org/article/227252fa3ef94c6da3e507f46f201022 |
op_doi |
https://doi.org/10.3390/s130303530 |
container_title |
Sensors |
container_volume |
13 |
container_issue |
3 |
container_start_page |
3530 |
op_container_end_page |
3548 |
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1766332079649325056 |