DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL
The objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological...
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ftdoajarticles:oai:doaj.org/article:226a1b7cec4e4bd6985d15bef7221e8a 2024-09-09T19:25:11+00:00 DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL Francisca Gleire Rodrigues de Menezes Soraya da Silva Neves Oscarina Viana de Sousa Candida Machado Vieira Maia Vila-Nova Rodrigo Maggioni Grace Nazareth Diogo Theophilo Ernesto Hofer Regine Helena Silva dos Fernandes Vieira 2014-09-01T00:00:00Z https://doi.org/10.1590/S0036-46652014000500010 https://doaj.org/article/226a1b7cec4e4bd6985d15bef7221e8a EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000500427&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000500010 https://doaj.org/article/226a1b7cec4e4bd6985d15bef7221e8a Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 5, Pp 427-432 (2014) Cholera Estuaries Pathogenicity Genes Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2014 ftdoajarticles https://doi.org/10.1590/S0036-46652014000500010 2024-08-05T17:49:30Z The objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological resemblance to V. cholerae were isolated. The cultures were identified phenotypically using a dichotomous key based on biochemical characteristics. The total DNA extracted was amplified by PCR to detect ompW and by multiplex PCR to detect the virulence genes ctx, tcp, zot and rfbO1. The results of the phenotypic and genotypic identification were compared. Nine strains of V. cholerae were identified phenotypically, five of which were confirmed by detection of the species-specific gene ompW. The dichotomous key was efficient at differentiating environmental strains of V. cholerae. Strains of V. cholerae were found in all four estuaries, but none possessed virulence genes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 56 5 427 432 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cholera Estuaries Pathogenicity Genes Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Cholera Estuaries Pathogenicity Genes Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Francisca Gleire Rodrigues de Menezes Soraya da Silva Neves Oscarina Viana de Sousa Candida Machado Vieira Maia Vila-Nova Rodrigo Maggioni Grace Nazareth Diogo Theophilo Ernesto Hofer Regine Helena Silva dos Fernandes Vieira DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL |
topic_facet |
Cholera Estuaries Pathogenicity Genes Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological resemblance to V. cholerae were isolated. The cultures were identified phenotypically using a dichotomous key based on biochemical characteristics. The total DNA extracted was amplified by PCR to detect ompW and by multiplex PCR to detect the virulence genes ctx, tcp, zot and rfbO1. The results of the phenotypic and genotypic identification were compared. Nine strains of V. cholerae were identified phenotypically, five of which were confirmed by detection of the species-specific gene ompW. The dichotomous key was efficient at differentiating environmental strains of V. cholerae. Strains of V. cholerae were found in all four estuaries, but none possessed virulence genes. |
format |
Article in Journal/Newspaper |
author |
Francisca Gleire Rodrigues de Menezes Soraya da Silva Neves Oscarina Viana de Sousa Candida Machado Vieira Maia Vila-Nova Rodrigo Maggioni Grace Nazareth Diogo Theophilo Ernesto Hofer Regine Helena Silva dos Fernandes Vieira |
author_facet |
Francisca Gleire Rodrigues de Menezes Soraya da Silva Neves Oscarina Viana de Sousa Candida Machado Vieira Maia Vila-Nova Rodrigo Maggioni Grace Nazareth Diogo Theophilo Ernesto Hofer Regine Helena Silva dos Fernandes Vieira |
author_sort |
Francisca Gleire Rodrigues de Menezes |
title |
DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL |
title_short |
DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL |
title_full |
DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL |
title_fullStr |
DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL |
title_full_unstemmed |
DETECTION OF VIRULENCE GENES IN ENVIRONMENTAL STRAINS OF Vibrio cholerae FROM ESTUARIES IN NORTHEASTERN BRAZIL |
title_sort |
detection of virulence genes in environmental strains of vibrio cholerae from estuaries in northeastern brazil |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2014 |
url |
https://doi.org/10.1590/S0036-46652014000500010 https://doaj.org/article/226a1b7cec4e4bd6985d15bef7221e8a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 56, Iss 5, Pp 427-432 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652014000500427&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652014000500010 https://doaj.org/article/226a1b7cec4e4bd6985d15bef7221e8a |
op_doi |
https://doi.org/10.1590/S0036-46652014000500010 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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56 |
container_issue |
5 |
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427 |
op_container_end_page |
432 |
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