Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer
During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during...
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ftdoajarticles:oai:doaj.org/article:2261349d8e3f40ccb23712a35f54e22a 2023-05-15T14:43:18+02:00 Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer S. Balzano P. Gourvil R. Siano M. Chanoine D. Marie S. Lessard D. Sarno D. Vaulot 2012-11-01T00:00:00Z https://doi.org/10.5194/bg-9-4553-2012 https://doaj.org/article/2261349d8e3f40ccb23712a35f54e22a EN eng Copernicus Publications http://www.biogeosciences.net/9/4553/2012/bg-9-4553-2012.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-9-4553-2012 1726-4170 1726-4189 https://doaj.org/article/2261349d8e3f40ccb23712a35f54e22a Biogeosciences, Vol 9, Iss 11, Pp 4553-4571 (2012) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2012 ftdoajarticles https://doi.org/10.5194/bg-9-4553-2012 2022-12-31T01:24:18Z During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during the summer season, strains were isolated by flow cytometry sorting (FCS) and single cell pipetting before or after phytoplankton enrichment of seawater samples. Strains were isolated both onboard and back in the laboratory and cultured at 4 °C under light/dark conditions. Overall, we isolated and characterised by light microscopy and 18 S rRNA gene sequencing 104 strains of photosynthetic flagellates which grouped into 21 genotypes (defined by 99.5% 18 S rRNA gene sequence similarity), mainly affiliated to Chlorophyta and Heterokontophyta. The taxon most frequently isolated was an Arctic ecotype of the green algal genus Micromonas (Arctic Micromonas ), which was nearly the only phytoplankter recovered within the picoplankton (< 2 μm) size range. Strains of Arctic Micromonas as well as other strains from the same class (Mamiellophyceae) were identified in further detail by sequencing the internal transcribed spacer (ITS) region of the rRNA operon. The MALINA Micromonas strains share identical 18 S rRNA and ITS sequences suggesting high genetic homogeneity within Arctic Micromonas . Three other Mamiellophyceae strains likely belong to a new genus. Other green algae from the genera Nephroselmis, Chlamydomonas , and Pyramimonas were also isolated, whereas Heterokontophyta included some unidentified Pelagophyceae, Dictyochophyceae (Pedinellales), and Chrysophyceae ( Dinobryon faculiferum ). Moreover, we isolated some Cryptophyceae ( Rhodomonas sp.) as well as a few Prymnesiophyceae and dinoflagellates. We identified the dinoflagellate Woloszynskia cincta by scanning electron microscopy (SEM) and 28 S rRNA gene sequencing. Our morphological analyses show that this species possess the diagnostic features of the genus ... Article in Journal/Newspaper Arctic Beaufort Sea Bering Strait Chukchi Chukchi Sea Phytoplankton Directory of Open Access Journals: DOAJ Articles Arctic Bering Strait Chukchi Sea Pacific Biogeosciences 9 11 4553 4571 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 S. Balzano P. Gourvil R. Siano M. Chanoine D. Marie S. Lessard D. Sarno D. Vaulot Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during the summer season, strains were isolated by flow cytometry sorting (FCS) and single cell pipetting before or after phytoplankton enrichment of seawater samples. Strains were isolated both onboard and back in the laboratory and cultured at 4 °C under light/dark conditions. Overall, we isolated and characterised by light microscopy and 18 S rRNA gene sequencing 104 strains of photosynthetic flagellates which grouped into 21 genotypes (defined by 99.5% 18 S rRNA gene sequence similarity), mainly affiliated to Chlorophyta and Heterokontophyta. The taxon most frequently isolated was an Arctic ecotype of the green algal genus Micromonas (Arctic Micromonas ), which was nearly the only phytoplankter recovered within the picoplankton (< 2 μm) size range. Strains of Arctic Micromonas as well as other strains from the same class (Mamiellophyceae) were identified in further detail by sequencing the internal transcribed spacer (ITS) region of the rRNA operon. The MALINA Micromonas strains share identical 18 S rRNA and ITS sequences suggesting high genetic homogeneity within Arctic Micromonas . Three other Mamiellophyceae strains likely belong to a new genus. Other green algae from the genera Nephroselmis, Chlamydomonas , and Pyramimonas were also isolated, whereas Heterokontophyta included some unidentified Pelagophyceae, Dictyochophyceae (Pedinellales), and Chrysophyceae ( Dinobryon faculiferum ). Moreover, we isolated some Cryptophyceae ( Rhodomonas sp.) as well as a few Prymnesiophyceae and dinoflagellates. We identified the dinoflagellate Woloszynskia cincta by scanning electron microscopy (SEM) and 28 S rRNA gene sequencing. Our morphological analyses show that this species possess the diagnostic features of the genus ... |
format |
Article in Journal/Newspaper |
author |
S. Balzano P. Gourvil R. Siano M. Chanoine D. Marie S. Lessard D. Sarno D. Vaulot |
author_facet |
S. Balzano P. Gourvil R. Siano M. Chanoine D. Marie S. Lessard D. Sarno D. Vaulot |
author_sort |
S. Balzano |
title |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_short |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_full |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_fullStr |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_full_unstemmed |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_sort |
diversity of cultured photosynthetic flagellates in the northeast pacific and arctic oceans in summer |
publisher |
Copernicus Publications |
publishDate |
2012 |
url |
https://doi.org/10.5194/bg-9-4553-2012 https://doaj.org/article/2261349d8e3f40ccb23712a35f54e22a |
geographic |
Arctic Bering Strait Chukchi Sea Pacific |
geographic_facet |
Arctic Bering Strait Chukchi Sea Pacific |
genre |
Arctic Beaufort Sea Bering Strait Chukchi Chukchi Sea Phytoplankton |
genre_facet |
Arctic Beaufort Sea Bering Strait Chukchi Chukchi Sea Phytoplankton |
op_source |
Biogeosciences, Vol 9, Iss 11, Pp 4553-4571 (2012) |
op_relation |
http://www.biogeosciences.net/9/4553/2012/bg-9-4553-2012.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-9-4553-2012 1726-4170 1726-4189 https://doaj.org/article/2261349d8e3f40ccb23712a35f54e22a |
op_doi |
https://doi.org/10.5194/bg-9-4553-2012 |
container_title |
Biogeosciences |
container_volume |
9 |
container_issue |
11 |
container_start_page |
4553 |
op_container_end_page |
4571 |
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1766314975262932992 |