Genomic Sequencing and Phylogenomics of Cowpox Virus
Cowpox virus (CPXV; genus Orthopoxvirus family Poxviridae ) is the causative agent of cowpox, a self-limiting zoonotic infection. CPXV is endemic in Eurasia, and human CPXV infections are associated with exposure to infected animals. In the Fennoscandian region, five CPXVs isolated from cats and hum...
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ftdoajarticles:oai:doaj.org/article:224365d3e7cc4a77bc9fcf37706124a2 2023-05-15T16:12:50+02:00 Genomic Sequencing and Phylogenomics of Cowpox Virus Diana Diaz-Cánova Carla Mavian Annika Brinkmann Andreas Nitsche Ugo Moens Malachy Ifeanyi Okeke 2022-09-01T00:00:00Z https://doi.org/10.3390/v14102134 https://doaj.org/article/224365d3e7cc4a77bc9fcf37706124a2 EN eng MDPI AG https://www.mdpi.com/1999-4915/14/10/2134 https://doaj.org/toc/1999-4915 doi:10.3390/v14102134 1999-4915 https://doaj.org/article/224365d3e7cc4a77bc9fcf37706124a2 Viruses, Vol 14, Iss 2134, p 2134 (2022) phylogenetic orthopoxvirus poxviridae molecular clock Fennoscandian phylodynamics Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3390/v14102134 2022-12-30T23:06:09Z Cowpox virus (CPXV; genus Orthopoxvirus family Poxviridae ) is the causative agent of cowpox, a self-limiting zoonotic infection. CPXV is endemic in Eurasia, and human CPXV infections are associated with exposure to infected animals. In the Fennoscandian region, five CPXVs isolated from cats and humans were collected and used in this study. We report the complete sequence of their genomes, which ranged in size from 220–222 kbp, containing between 215 and 219 open reading frames. The phylogenetic analysis of 87 orthopoxvirus strains, including the Fennoscandian CPXV isolates, confirmed the division of CPXV strains into at least five distinct major clusters (CPXV-like 1, CPXV-like 2, VACV-like, VARV-like and ECTV-Abatino-like) and can be further divided into eighteen sub-species based on the genetic and patristic distances. Bayesian time-scaled evolutionary history of CPXV was reconstructed employing concatenated 62 non-recombinant conserved genes of 55 CPXV. The CPXV evolution rate was calculated to be 1.65 × 10 −5 substitution/site/year. Our findings confirmed that CPXV is not a single species but a polyphyletic assemblage of several species and thus, a reclassification is warranted. Article in Journal/Newspaper Fennoscandian Directory of Open Access Journals: DOAJ Articles Viruses 14 10 2134 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
phylogenetic orthopoxvirus poxviridae molecular clock Fennoscandian phylodynamics Microbiology QR1-502 |
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phylogenetic orthopoxvirus poxviridae molecular clock Fennoscandian phylodynamics Microbiology QR1-502 Diana Diaz-Cánova Carla Mavian Annika Brinkmann Andreas Nitsche Ugo Moens Malachy Ifeanyi Okeke Genomic Sequencing and Phylogenomics of Cowpox Virus |
topic_facet |
phylogenetic orthopoxvirus poxviridae molecular clock Fennoscandian phylodynamics Microbiology QR1-502 |
description |
Cowpox virus (CPXV; genus Orthopoxvirus family Poxviridae ) is the causative agent of cowpox, a self-limiting zoonotic infection. CPXV is endemic in Eurasia, and human CPXV infections are associated with exposure to infected animals. In the Fennoscandian region, five CPXVs isolated from cats and humans were collected and used in this study. We report the complete sequence of their genomes, which ranged in size from 220–222 kbp, containing between 215 and 219 open reading frames. The phylogenetic analysis of 87 orthopoxvirus strains, including the Fennoscandian CPXV isolates, confirmed the division of CPXV strains into at least five distinct major clusters (CPXV-like 1, CPXV-like 2, VACV-like, VARV-like and ECTV-Abatino-like) and can be further divided into eighteen sub-species based on the genetic and patristic distances. Bayesian time-scaled evolutionary history of CPXV was reconstructed employing concatenated 62 non-recombinant conserved genes of 55 CPXV. The CPXV evolution rate was calculated to be 1.65 × 10 −5 substitution/site/year. Our findings confirmed that CPXV is not a single species but a polyphyletic assemblage of several species and thus, a reclassification is warranted. |
format |
Article in Journal/Newspaper |
author |
Diana Diaz-Cánova Carla Mavian Annika Brinkmann Andreas Nitsche Ugo Moens Malachy Ifeanyi Okeke |
author_facet |
Diana Diaz-Cánova Carla Mavian Annika Brinkmann Andreas Nitsche Ugo Moens Malachy Ifeanyi Okeke |
author_sort |
Diana Diaz-Cánova |
title |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_short |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_full |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_fullStr |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_full_unstemmed |
Genomic Sequencing and Phylogenomics of Cowpox Virus |
title_sort |
genomic sequencing and phylogenomics of cowpox virus |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/v14102134 https://doaj.org/article/224365d3e7cc4a77bc9fcf37706124a2 |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_source |
Viruses, Vol 14, Iss 2134, p 2134 (2022) |
op_relation |
https://www.mdpi.com/1999-4915/14/10/2134 https://doaj.org/toc/1999-4915 doi:10.3390/v14102134 1999-4915 https://doaj.org/article/224365d3e7cc4a77bc9fcf37706124a2 |
op_doi |
https://doi.org/10.3390/v14102134 |
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Viruses |
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14 |
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10 |
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2134 |
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1765998443933728768 |