ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data
Metagenomic analysis of environmental samples provides deep insight into the enzymatic mixture of the corresponding niches, capable of revealing peptide sequences with novel functional properties exploiting the high performance of next-generation sequencing (NGS) technologies. At the same time due t...
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ftdoajarticles:oai:doaj.org/article:222339b77c634d27950e282d8f5e596d 2023-05-15T16:51:13+02:00 ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data Theodoros Koutsandreas Efthymios Ladoukakis Eleftherios Pilalis Dimitra Zarafeta Fragiskos N. Kolisis Georgios Skretas Aristotelis A. Chatziioannou 2019-05-01T00:00:00Z https://doi.org/10.3389/fgene.2019.00469 https://doaj.org/article/222339b77c634d27950e282d8f5e596d EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fgene.2019.00469/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2019.00469 https://doaj.org/article/222339b77c634d27950e282d8f5e596d Frontiers in Genetics, Vol 10 (2019) metagenomics bioinformatics next generation sequencing automated pipelines systemic biology novel enzymes Genetics QH426-470 article 2019 ftdoajarticles https://doi.org/10.3389/fgene.2019.00469 2022-12-31T13:44:07Z Metagenomic analysis of environmental samples provides deep insight into the enzymatic mixture of the corresponding niches, capable of revealing peptide sequences with novel functional properties exploiting the high performance of next-generation sequencing (NGS) technologies. At the same time due to their ever increasing complexity, there is a compelling need for ever larger computational configurations to ensure proper bioinformatic analysis, and fine annotation. With the aiming to address the challenges of such an endeavor, we have developed a novel web-based application named ANASTASIA (automated nucleotide aminoacid sequences translational plAtform for systemic interpretation and analysis). ANASTASIA provides a rich environment of bioinformatic tools, either publicly available or novel, proprietary algorithms, integrated within numerous automated algorithmic workflows, and which enables versatile data processing tasks for (meta)genomic sequence datasets. ANASTASIA was initially developed in the framework of the European FP7 project HotZyme, whose aim was to perform exhaustive analysis of metagenomes derived from thermal springs around the globe and to discover new enzymes of industrial interest. ANASTASIA has evolved to become a stable and extensible environment for diversified, metagenomic, functional analyses for a range of applications overarching industrial biotechnology to biomedicine, within the frames of the ELIXIR-GR project. As a showcase, we report the successful in silico mining of a novel thermostable esterase termed “EstDZ4” from a metagenomic sample collected from a hot spring located in Krisuvik, Iceland. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles Frontiers in Genetics 10 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
metagenomics bioinformatics next generation sequencing automated pipelines systemic biology novel enzymes Genetics QH426-470 |
spellingShingle |
metagenomics bioinformatics next generation sequencing automated pipelines systemic biology novel enzymes Genetics QH426-470 Theodoros Koutsandreas Efthymios Ladoukakis Eleftherios Pilalis Dimitra Zarafeta Fragiskos N. Kolisis Georgios Skretas Aristotelis A. Chatziioannou ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data |
topic_facet |
metagenomics bioinformatics next generation sequencing automated pipelines systemic biology novel enzymes Genetics QH426-470 |
description |
Metagenomic analysis of environmental samples provides deep insight into the enzymatic mixture of the corresponding niches, capable of revealing peptide sequences with novel functional properties exploiting the high performance of next-generation sequencing (NGS) technologies. At the same time due to their ever increasing complexity, there is a compelling need for ever larger computational configurations to ensure proper bioinformatic analysis, and fine annotation. With the aiming to address the challenges of such an endeavor, we have developed a novel web-based application named ANASTASIA (automated nucleotide aminoacid sequences translational plAtform for systemic interpretation and analysis). ANASTASIA provides a rich environment of bioinformatic tools, either publicly available or novel, proprietary algorithms, integrated within numerous automated algorithmic workflows, and which enables versatile data processing tasks for (meta)genomic sequence datasets. ANASTASIA was initially developed in the framework of the European FP7 project HotZyme, whose aim was to perform exhaustive analysis of metagenomes derived from thermal springs around the globe and to discover new enzymes of industrial interest. ANASTASIA has evolved to become a stable and extensible environment for diversified, metagenomic, functional analyses for a range of applications overarching industrial biotechnology to biomedicine, within the frames of the ELIXIR-GR project. As a showcase, we report the successful in silico mining of a novel thermostable esterase termed “EstDZ4” from a metagenomic sample collected from a hot spring located in Krisuvik, Iceland. |
format |
Article in Journal/Newspaper |
author |
Theodoros Koutsandreas Efthymios Ladoukakis Eleftherios Pilalis Dimitra Zarafeta Fragiskos N. Kolisis Georgios Skretas Aristotelis A. Chatziioannou |
author_facet |
Theodoros Koutsandreas Efthymios Ladoukakis Eleftherios Pilalis Dimitra Zarafeta Fragiskos N. Kolisis Georgios Skretas Aristotelis A. Chatziioannou |
author_sort |
Theodoros Koutsandreas |
title |
ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data |
title_short |
ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data |
title_full |
ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data |
title_fullStr |
ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data |
title_full_unstemmed |
ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data |
title_sort |
anastasia: an automated metagenomic analysis pipeline for novel enzyme discovery exploiting next generation sequencing data |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fgene.2019.00469 https://doaj.org/article/222339b77c634d27950e282d8f5e596d |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Frontiers in Genetics, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fgene.2019.00469/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2019.00469 https://doaj.org/article/222339b77c634d27950e282d8f5e596d |
op_doi |
https://doi.org/10.3389/fgene.2019.00469 |
container_title |
Frontiers in Genetics |
container_volume |
10 |
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1766041333786476544 |