Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America
Abstract Background Malaria is an important disease in many tropical countries. Rapid diagnostic tests (RDTs) are valuable tools for diagnosing malaria in remote areas. The majority of RDTs used for the diagnosis of Plasmodium falciparum are based on the detection of the specific histidine-rich prot...
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ftdoajarticles:oai:doaj.org/article:2208e4d966d043b5a6a4c1dc905959ec 2023-05-15T15:11:25+02:00 Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America Gustavo Fontecha Alejandra Pinto Denis Escobar Gabriela Matamoros Bryan Ortiz 2019-01-01T00:00:00Z https://doi.org/10.1186/s12936-019-2668-3 https://doaj.org/article/2208e4d966d043b5a6a4c1dc905959ec EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2668-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2668-3 1475-2875 https://doaj.org/article/2208e4d966d043b5a6a4c1dc905959ec Malaria Journal, Vol 18, Iss 1, Pp 1-10 (2019) Malaria pfhrp2 pfhrp3 Plasmodium falciparum Rapid diagnostic test Honduras Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2668-3 2022-12-31T03:10:32Z Abstract Background Malaria is an important disease in many tropical countries. Rapid diagnostic tests (RDTs) are valuable tools for diagnosing malaria in remote areas. The majority of RDTs used for the diagnosis of Plasmodium falciparum are based on the detection of the specific histidine-rich proteins (PfHRP2 and PfHRP3). During the last decade, the threat posed by the lack of expression of these antigens and the variability of the proteins on the diagnosis of malaria has been widely discussed. The aim of this study was to evaluate the genetic diversity of pfhrp2 and pfhrp3 of P. falciparum isolates collected in three Central American countries. Methods DNA samples were amplified and sequenced to assess the diversity of nucleotides and amino acids. A search for known epitopes within the amino acid sequence was carried out, and the sensitivity of the sequences was evaluated according to a predictive model. A phylogenetic analysis was carried out including homologous sequences from different regions of the world. Protein structures were predicted in silico. Results Five different patterns for PfHRP2 and one pattern for PfHRP3 were identified. Isolates from Central America show a high level of genetic diversity in pfhrp2; however, the amino acid sequences seem to contain enough motifs to be detected by the RDTs currently available. Conclusion It is unlikely that the variability of the pfhrp2 and pfhrp3 genes has a significant impact on the ability of the RDTs to detect the PfHRP antigens in Central America. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
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Malaria pfhrp2 pfhrp3 Plasmodium falciparum Rapid diagnostic test Honduras Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria pfhrp2 pfhrp3 Plasmodium falciparum Rapid diagnostic test Honduras Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Gustavo Fontecha Alejandra Pinto Denis Escobar Gabriela Matamoros Bryan Ortiz Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America |
topic_facet |
Malaria pfhrp2 pfhrp3 Plasmodium falciparum Rapid diagnostic test Honduras Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is an important disease in many tropical countries. Rapid diagnostic tests (RDTs) are valuable tools for diagnosing malaria in remote areas. The majority of RDTs used for the diagnosis of Plasmodium falciparum are based on the detection of the specific histidine-rich proteins (PfHRP2 and PfHRP3). During the last decade, the threat posed by the lack of expression of these antigens and the variability of the proteins on the diagnosis of malaria has been widely discussed. The aim of this study was to evaluate the genetic diversity of pfhrp2 and pfhrp3 of P. falciparum isolates collected in three Central American countries. Methods DNA samples were amplified and sequenced to assess the diversity of nucleotides and amino acids. A search for known epitopes within the amino acid sequence was carried out, and the sensitivity of the sequences was evaluated according to a predictive model. A phylogenetic analysis was carried out including homologous sequences from different regions of the world. Protein structures were predicted in silico. Results Five different patterns for PfHRP2 and one pattern for PfHRP3 were identified. Isolates from Central America show a high level of genetic diversity in pfhrp2; however, the amino acid sequences seem to contain enough motifs to be detected by the RDTs currently available. Conclusion It is unlikely that the variability of the pfhrp2 and pfhrp3 genes has a significant impact on the ability of the RDTs to detect the PfHRP antigens in Central America. |
format |
Article in Journal/Newspaper |
author |
Gustavo Fontecha Alejandra Pinto Denis Escobar Gabriela Matamoros Bryan Ortiz |
author_facet |
Gustavo Fontecha Alejandra Pinto Denis Escobar Gabriela Matamoros Bryan Ortiz |
author_sort |
Gustavo Fontecha |
title |
Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America |
title_short |
Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America |
title_full |
Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America |
title_fullStr |
Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America |
title_full_unstemmed |
Genetic variability of Plasmodium falciparum histidine-rich proteins 2 and 3 in Central America |
title_sort |
genetic variability of plasmodium falciparum histidine-rich proteins 2 and 3 in central america |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2668-3 https://doaj.org/article/2208e4d966d043b5a6a4c1dc905959ec |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-10 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2668-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2668-3 1475-2875 https://doaj.org/article/2208e4d966d043b5a6a4c1dc905959ec |
op_doi |
https://doi.org/10.1186/s12936-019-2668-3 |
container_title |
Malaria Journal |
container_volume |
18 |
container_issue |
1 |
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1766342269748641792 |