Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone
Abstract Background Rapid and sensitive diagnostics are critical tools for clinical case management and public health control efforts. Both capillary and venous blood are currently used for malaria detection and while diagnostic technologies may not be equally sensitive with both materials, the publ...
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ftdoajarticles:oai:doaj.org/article:21fffca5dff54d4da3faa56279f0c0d3 2023-05-15T15:08:46+02:00 Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone Tomasz A. Leski Chris Rowe Taitt Umaru Bangura Joseph Lahai Joseph M. Lamin Victoria Baio Mohamed S. Koroma Abdulai G. Swaray Kathryn H. Jacobsen Olivia Jackson Brian W. Jones Cynthia L. Phillips Rashid Ansumana David A. Stenger 2021-03-01T00:00:00Z https://doi.org/10.1186/s12936-021-03644-y https://doaj.org/article/21fffca5dff54d4da3faa56279f0c0d3 EN eng BMC https://doi.org/10.1186/s12936-021-03644-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03644-y 1475-2875 https://doaj.org/article/21fffca5dff54d4da3faa56279f0c0d3 Malaria Journal, Vol 20, Iss 1, Pp 1-9 (2021) Malaria Capillary blood Venous blood Multiplex polymerase chain reaction Plasmodium Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03644-y 2022-12-31T10:19:38Z Abstract Background Rapid and sensitive diagnostics are critical tools for clinical case management and public health control efforts. Both capillary and venous blood are currently used for malaria detection and while diagnostic technologies may not be equally sensitive with both materials, the published data on this subject are scarce and not conclusive. Methods Paired clinical samples of venous and capillary blood from 141 febrile individuals in Bo, Sierra Leone, were obtained between January and May 2019 and tested for the presence of Plasmodium parasites using two multiplexed PCR assays: the FilmArray-based Global Fever Panel (GFP) and the TaqMan-based Malaria Multiplex Sample Ready (MMSR) assay. Results No significant differences in Plasmodium parasite detection between capillary and venous blood for both assays were observed. The GFP assay was more sensitive than MMSR for all markers that could be compared (Plasmodium spp. and Plasmodium falciparum) in both venous and capillary blood. Conclusions No difference was found in malaria detection between venous and capillary blood using two different PCR-based detection assays. This data gives support for use of capillary blood, a material which can be obtained easier by less invasive methods, for PCR-based malaria diagnostics, independent of the platform. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria Capillary blood Venous blood Multiplex polymerase chain reaction Plasmodium Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Capillary blood Venous blood Multiplex polymerase chain reaction Plasmodium Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Tomasz A. Leski Chris Rowe Taitt Umaru Bangura Joseph Lahai Joseph M. Lamin Victoria Baio Mohamed S. Koroma Abdulai G. Swaray Kathryn H. Jacobsen Olivia Jackson Brian W. Jones Cynthia L. Phillips Rashid Ansumana David A. Stenger Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone |
topic_facet |
Malaria Capillary blood Venous blood Multiplex polymerase chain reaction Plasmodium Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Rapid and sensitive diagnostics are critical tools for clinical case management and public health control efforts. Both capillary and venous blood are currently used for malaria detection and while diagnostic technologies may not be equally sensitive with both materials, the published data on this subject are scarce and not conclusive. Methods Paired clinical samples of venous and capillary blood from 141 febrile individuals in Bo, Sierra Leone, were obtained between January and May 2019 and tested for the presence of Plasmodium parasites using two multiplexed PCR assays: the FilmArray-based Global Fever Panel (GFP) and the TaqMan-based Malaria Multiplex Sample Ready (MMSR) assay. Results No significant differences in Plasmodium parasite detection between capillary and venous blood for both assays were observed. The GFP assay was more sensitive than MMSR for all markers that could be compared (Plasmodium spp. and Plasmodium falciparum) in both venous and capillary blood. Conclusions No difference was found in malaria detection between venous and capillary blood using two different PCR-based detection assays. This data gives support for use of capillary blood, a material which can be obtained easier by less invasive methods, for PCR-based malaria diagnostics, independent of the platform. |
format |
Article in Journal/Newspaper |
author |
Tomasz A. Leski Chris Rowe Taitt Umaru Bangura Joseph Lahai Joseph M. Lamin Victoria Baio Mohamed S. Koroma Abdulai G. Swaray Kathryn H. Jacobsen Olivia Jackson Brian W. Jones Cynthia L. Phillips Rashid Ansumana David A. Stenger |
author_facet |
Tomasz A. Leski Chris Rowe Taitt Umaru Bangura Joseph Lahai Joseph M. Lamin Victoria Baio Mohamed S. Koroma Abdulai G. Swaray Kathryn H. Jacobsen Olivia Jackson Brian W. Jones Cynthia L. Phillips Rashid Ansumana David A. Stenger |
author_sort |
Tomasz A. Leski |
title |
Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone |
title_short |
Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone |
title_full |
Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone |
title_fullStr |
Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone |
title_full_unstemmed |
Comparison of capillary and venous blood for malaria detection using two PCR-based assays in febrile patients in Sierra Leone |
title_sort |
comparison of capillary and venous blood for malaria detection using two pcr-based assays in febrile patients in sierra leone |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03644-y https://doaj.org/article/21fffca5dff54d4da3faa56279f0c0d3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-9 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03644-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03644-y 1475-2875 https://doaj.org/article/21fffca5dff54d4da3faa56279f0c0d3 |
op_doi |
https://doi.org/10.1186/s12936-021-03644-y |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
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1766340073498869760 |