Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates.
Since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. Tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplicatio...
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ftdoajarticles:oai:doaj.org/article:21ff4c90a9a2438885f8f349b85b27ec 2023-05-15T16:08:41+02:00 Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. Marina Morini Jérémy Pasquier Ron Dirks Guido van den Thillart Jonna Tomkiewicz Karine Rousseau Sylvie Dufour Anne-Gaëlle Lafont 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0126008 https://doaj.org/article/21ff4c90a9a2438885f8f349b85b27ec EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0126008 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0126008 https://doaj.org/article/21ff4c90a9a2438885f8f349b85b27ec PLoS ONE, Vol 10, Iss 5, p e0126008 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0126008 2022-12-31T13:17:12Z Since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. Tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplication event (3R). Leptin acts through a specific receptor (LEPR). In the European and Japanese eels, we identified two leptin genes, and for the first time in vertebrates, two LEPR genes. Synteny analyses indicated that eel LEPRa and LEPRb result from teleost 3R. LEPRb seems to have been lost in the teleost lineage shortly after the elopomorph divergence. Quantitative PCRs revealed a wide distribution of leptins and LEPRs in the European eel, including tissues involved in metabolism and reproduction. Noticeably, leptin1 was expressed in fat tissue, while leptin2 in the liver, reflecting subfunctionalization. Four-month fasting had no impact on the expression of leptins and LEPRs in control European eels. This might be related to the remarkable adaptation of silver eel metabolism to long-term fasting throughout the reproductive oceanic migration. In contrast, sexual maturation induced differential increases in the expression of leptins and LEPRs in the BPG-liver axis. Leptin2 was strikingly upregulated in the liver, the central organ of the reproductive metabolic challenge in teleosts. LEPRs were differentially regulated during sexual maturation, which may have contributed to the conservation of the duplicated LEPRs in this species. This suggests an ancient and positive role of the leptin system in the vertebrate reproductive function. This study brings new insights on the evolutionary history of the leptin system in vertebrates. Among extant vertebrates, the eel represents a unique case of duplicated leptins and leptin receptors as a result of 3R. Article in Journal/Newspaper European eel Directory of Open Access Journals: DOAJ Articles PLOS ONE 10 5 e0126008 |
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Medicine R Science Q Marina Morini Jérémy Pasquier Ron Dirks Guido van den Thillart Jonna Tomkiewicz Karine Rousseau Sylvie Dufour Anne-Gaëlle Lafont Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. |
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Medicine R Science Q |
description |
Since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. Tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplication event (3R). Leptin acts through a specific receptor (LEPR). In the European and Japanese eels, we identified two leptin genes, and for the first time in vertebrates, two LEPR genes. Synteny analyses indicated that eel LEPRa and LEPRb result from teleost 3R. LEPRb seems to have been lost in the teleost lineage shortly after the elopomorph divergence. Quantitative PCRs revealed a wide distribution of leptins and LEPRs in the European eel, including tissues involved in metabolism and reproduction. Noticeably, leptin1 was expressed in fat tissue, while leptin2 in the liver, reflecting subfunctionalization. Four-month fasting had no impact on the expression of leptins and LEPRs in control European eels. This might be related to the remarkable adaptation of silver eel metabolism to long-term fasting throughout the reproductive oceanic migration. In contrast, sexual maturation induced differential increases in the expression of leptins and LEPRs in the BPG-liver axis. Leptin2 was strikingly upregulated in the liver, the central organ of the reproductive metabolic challenge in teleosts. LEPRs were differentially regulated during sexual maturation, which may have contributed to the conservation of the duplicated LEPRs in this species. This suggests an ancient and positive role of the leptin system in the vertebrate reproductive function. This study brings new insights on the evolutionary history of the leptin system in vertebrates. Among extant vertebrates, the eel represents a unique case of duplicated leptins and leptin receptors as a result of 3R. |
format |
Article in Journal/Newspaper |
author |
Marina Morini Jérémy Pasquier Ron Dirks Guido van den Thillart Jonna Tomkiewicz Karine Rousseau Sylvie Dufour Anne-Gaëlle Lafont |
author_facet |
Marina Morini Jérémy Pasquier Ron Dirks Guido van den Thillart Jonna Tomkiewicz Karine Rousseau Sylvie Dufour Anne-Gaëlle Lafont |
author_sort |
Marina Morini |
title |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. |
title_short |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. |
title_full |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. |
title_fullStr |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. |
title_full_unstemmed |
Duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. |
title_sort |
duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0126008 https://doaj.org/article/21ff4c90a9a2438885f8f349b85b27ec |
genre |
European eel |
genre_facet |
European eel |
op_source |
PLoS ONE, Vol 10, Iss 5, p e0126008 (2015) |
op_relation |
https://doi.org/10.1371/journal.pone.0126008 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0126008 https://doaj.org/article/21ff4c90a9a2438885f8f349b85b27ec |
op_doi |
https://doi.org/10.1371/journal.pone.0126008 |
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PLOS ONE |
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10 |
container_issue |
5 |
container_start_page |
e0126008 |
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1766404701516988416 |