Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics
Objective: To analyze the feasibility of detecting Ph1 in leukemia patients in the Kinshasa University Clinics in the Democratic Republic of Congo, at KU Leuven, Belgium. Methods: Bone marrow and peripheral blood samples with chronic myeloid leukemia, acute myeloid leukemia or acute leukocytes leuke...
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2017.10.014 https://doaj.org/article/21e74badf7b1445bbf7199a99710237b |
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ftdoajarticles:oai:doaj.org/article:21e74badf7b1445bbf7199a99710237b 2023-05-15T15:06:58+02:00 Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics Mireille Solange Nganga Nkanga Benjamin Longo-Mbenza Peter Vandenberghe Fons Verdonck Venant Tchokonte-Nana Nkama Claude Nlandu Roth Laure Mapapa Miakassissa Paul Roger Beia Kazadi Antoine Lufimbo Katawandja Jacques Bikaula Ngwidiwo Ntolo Jean-Pierre Mufuta Kuanda Thomas Solo Mbombo Aurore Cecilia Orphée Beia 2017-12-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.10.014 https://doaj.org/article/21e74badf7b1445bbf7199a99710237b EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117309887 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.10.014 https://doaj.org/article/21e74badf7b1445bbf7199a99710237b Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 12, Pp 1116-1119 (2017) Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.10.014 2022-12-31T13:49:52Z Objective: To analyze the feasibility of detecting Ph1 in leukemia patients in the Kinshasa University Clinics in the Democratic Republic of Congo, at KU Leuven, Belgium. Methods: Bone marrow and peripheral blood samples with chronic myeloid leukemia, acute myeloid leukemia or acute leukocytes leukemia were obtained from 32 patients in Kinshasa University clinics in the Democratic Republic of Congo and transferred to KU Leuven in Belgium for iFISH feasibility. Ph1 was detected by using a remote analysis of interphase fluorescence in situ hybridization (iFISH). Results: Out of the 32 patients involved in this study, 65.6% (n = 21) of the cases were successfully tested, of which 52.4% (n = 11) were iFISH positives for the variant t(9;22) (presence of Ph1) in chronic myeloid leukemia samples and 47.6% (n = 10) negatives in all subtypes of hematological malignancies. However, there was a female predominance in chronic myeloid leukemia samples Ph1-positives by iFISH, whereas no sexual influence was observed on acute subtypes of leukemia. Conclusions: iFISH analysis is feasible on samples obtained from remote sites in the Democratic Republic of Congo. However, the optimization of the sample storage is necessary to further improve iFISH's performance. Keywords: iFISH, Ph1, Democratic Republic of Congo, Leukemia, Bone marrow, Blood Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 12 1116 1119 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mireille Solange Nganga Nkanga Benjamin Longo-Mbenza Peter Vandenberghe Fons Verdonck Venant Tchokonte-Nana Nkama Claude Nlandu Roth Laure Mapapa Miakassissa Paul Roger Beia Kazadi Antoine Lufimbo Katawandja Jacques Bikaula Ngwidiwo Ntolo Jean-Pierre Mufuta Kuanda Thomas Solo Mbombo Aurore Cecilia Orphée Beia Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To analyze the feasibility of detecting Ph1 in leukemia patients in the Kinshasa University Clinics in the Democratic Republic of Congo, at KU Leuven, Belgium. Methods: Bone marrow and peripheral blood samples with chronic myeloid leukemia, acute myeloid leukemia or acute leukocytes leukemia were obtained from 32 patients in Kinshasa University clinics in the Democratic Republic of Congo and transferred to KU Leuven in Belgium for iFISH feasibility. Ph1 was detected by using a remote analysis of interphase fluorescence in situ hybridization (iFISH). Results: Out of the 32 patients involved in this study, 65.6% (n = 21) of the cases were successfully tested, of which 52.4% (n = 11) were iFISH positives for the variant t(9;22) (presence of Ph1) in chronic myeloid leukemia samples and 47.6% (n = 10) negatives in all subtypes of hematological malignancies. However, there was a female predominance in chronic myeloid leukemia samples Ph1-positives by iFISH, whereas no sexual influence was observed on acute subtypes of leukemia. Conclusions: iFISH analysis is feasible on samples obtained from remote sites in the Democratic Republic of Congo. However, the optimization of the sample storage is necessary to further improve iFISH's performance. Keywords: iFISH, Ph1, Democratic Republic of Congo, Leukemia, Bone marrow, Blood |
format |
Article in Journal/Newspaper |
author |
Mireille Solange Nganga Nkanga Benjamin Longo-Mbenza Peter Vandenberghe Fons Verdonck Venant Tchokonte-Nana Nkama Claude Nlandu Roth Laure Mapapa Miakassissa Paul Roger Beia Kazadi Antoine Lufimbo Katawandja Jacques Bikaula Ngwidiwo Ntolo Jean-Pierre Mufuta Kuanda Thomas Solo Mbombo Aurore Cecilia Orphée Beia |
author_facet |
Mireille Solange Nganga Nkanga Benjamin Longo-Mbenza Peter Vandenberghe Fons Verdonck Venant Tchokonte-Nana Nkama Claude Nlandu Roth Laure Mapapa Miakassissa Paul Roger Beia Kazadi Antoine Lufimbo Katawandja Jacques Bikaula Ngwidiwo Ntolo Jean-Pierre Mufuta Kuanda Thomas Solo Mbombo Aurore Cecilia Orphée Beia |
author_sort |
Mireille Solange Nganga Nkanga |
title |
Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics |
title_short |
Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics |
title_full |
Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics |
title_fullStr |
Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics |
title_full_unstemmed |
Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics |
title_sort |
feasibility of ifish patterns in hematologic malignancies among congolese patients at kinshasa university clinics |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.10.014 https://doaj.org/article/21e74badf7b1445bbf7199a99710237b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 12, Pp 1116-1119 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117309887 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.10.014 https://doaj.org/article/21e74badf7b1445bbf7199a99710237b |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.10.014 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
12 |
container_start_page |
1116 |
op_container_end_page |
1119 |
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1766338550652993536 |