Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae
The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to anti...
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ftdoajarticles:oai:doaj.org/article:21d125dd94c8428f88ac9832c5858ca3 2023-05-15T15:27:44+02:00 Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae Nanna Rørbo Anita Rønneseth Panos G. Kalatzis Bastian Barker Rasmussen Kirsten Engell-Sørensen Hans Petter Kleppen Heidrun Inger Wergeland Lone Gram Mathias Middelboe 2018-05-01T00:00:00Z https://doi.org/10.3390/antibiotics7020042 https://doaj.org/article/21d125dd94c8428f88ac9832c5858ca3 EN eng MDPI AG http://www.mdpi.com/2079-6382/7/2/42 https://doaj.org/toc/2079-6382 2079-6382 doi:10.3390/antibiotics7020042 https://doaj.org/article/21d125dd94c8428f88ac9832c5858ca3 Antibiotics, Vol 7, Iss 2, p 42 (2018) Vibrio anguillarum phage therapy aquaculture fish larvae challenge trials Therapeutics. Pharmacology RM1-950 article 2018 ftdoajarticles https://doi.org/10.3390/antibiotics7020042 2022-12-31T06:26:32Z The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to antibiotics and inefficient vaccination at the larval stage of fish emphasizes the need for novel approaches, and phage therapy for controlling Vibrio pathogens has gained interest in the past few years. In this study, we examined the potential of the broad-host-range phage KVP40 to control four different V. anguillarum strains in Atlantic cod (Gadus morhua L.) and turbot (Scophthalmus maximus L.) larvae. We examined larval mortality and abundance of bacteria and phages. Phage KVP40 was able to reduce and/or delay the mortality of the cod and turbot larvae challenged with V. anguillarum. However, growth of other pathogenic bacteria naturally occurring on the fish eggs prior to our experiment caused mortality of the larvae in the unchallenged control groups. Interestingly, the broad-spectrum phage KVP40 was able to reduce mortality in these groups, compared to the nonchallenge control groups not treated with phage KVP40, demonstrating that the phage could also reduce mortality imposed by the background population of pathogens. Overall, phage-mediated reduction in mortality of cod and turbot larvae in experimental challenge assays with V. anguillarum pathogens suggested that application of broad-host-range phages can reduce Vibrio-induced mortality in turbot and cod larvae, emphasizing that phage therapy is a promising alternative to traditional treatment of vibriosis in marine aquaculture. Article in Journal/Newspaper atlantic cod Gadus morhua Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles Antibiotics 7 2 42 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Vibrio anguillarum phage therapy aquaculture fish larvae challenge trials Therapeutics. Pharmacology RM1-950 |
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Vibrio anguillarum phage therapy aquaculture fish larvae challenge trials Therapeutics. Pharmacology RM1-950 Nanna Rørbo Anita Rønneseth Panos G. Kalatzis Bastian Barker Rasmussen Kirsten Engell-Sørensen Hans Petter Kleppen Heidrun Inger Wergeland Lone Gram Mathias Middelboe Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae |
topic_facet |
Vibrio anguillarum phage therapy aquaculture fish larvae challenge trials Therapeutics. Pharmacology RM1-950 |
description |
The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to antibiotics and inefficient vaccination at the larval stage of fish emphasizes the need for novel approaches, and phage therapy for controlling Vibrio pathogens has gained interest in the past few years. In this study, we examined the potential of the broad-host-range phage KVP40 to control four different V. anguillarum strains in Atlantic cod (Gadus morhua L.) and turbot (Scophthalmus maximus L.) larvae. We examined larval mortality and abundance of bacteria and phages. Phage KVP40 was able to reduce and/or delay the mortality of the cod and turbot larvae challenged with V. anguillarum. However, growth of other pathogenic bacteria naturally occurring on the fish eggs prior to our experiment caused mortality of the larvae in the unchallenged control groups. Interestingly, the broad-spectrum phage KVP40 was able to reduce mortality in these groups, compared to the nonchallenge control groups not treated with phage KVP40, demonstrating that the phage could also reduce mortality imposed by the background population of pathogens. Overall, phage-mediated reduction in mortality of cod and turbot larvae in experimental challenge assays with V. anguillarum pathogens suggested that application of broad-host-range phages can reduce Vibrio-induced mortality in turbot and cod larvae, emphasizing that phage therapy is a promising alternative to traditional treatment of vibriosis in marine aquaculture. |
format |
Article in Journal/Newspaper |
author |
Nanna Rørbo Anita Rønneseth Panos G. Kalatzis Bastian Barker Rasmussen Kirsten Engell-Sørensen Hans Petter Kleppen Heidrun Inger Wergeland Lone Gram Mathias Middelboe |
author_facet |
Nanna Rørbo Anita Rønneseth Panos G. Kalatzis Bastian Barker Rasmussen Kirsten Engell-Sørensen Hans Petter Kleppen Heidrun Inger Wergeland Lone Gram Mathias Middelboe |
author_sort |
Nanna Rørbo |
title |
Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae |
title_short |
Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae |
title_full |
Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae |
title_fullStr |
Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae |
title_full_unstemmed |
Exploring the Effect of Phage Therapy in Preventing Vibrio anguillarum Infections in Cod and Turbot Larvae |
title_sort |
exploring the effect of phage therapy in preventing vibrio anguillarum infections in cod and turbot larvae |
publisher |
MDPI AG |
publishDate |
2018 |
url |
https://doi.org/10.3390/antibiotics7020042 https://doaj.org/article/21d125dd94c8428f88ac9832c5858ca3 |
genre |
atlantic cod Gadus morhua Scophthalmus maximus Turbot |
genre_facet |
atlantic cod Gadus morhua Scophthalmus maximus Turbot |
op_source |
Antibiotics, Vol 7, Iss 2, p 42 (2018) |
op_relation |
http://www.mdpi.com/2079-6382/7/2/42 https://doaj.org/toc/2079-6382 2079-6382 doi:10.3390/antibiotics7020042 https://doaj.org/article/21d125dd94c8428f88ac9832c5858ca3 |
op_doi |
https://doi.org/10.3390/antibiotics7020042 |
container_title |
Antibiotics |
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7 |
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2 |
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42 |
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1766358147568500736 |