TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA
Toxocariasis is caused by infection of man by Toxocara canis and Toxocara cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp a...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:21cb720a4144465da58f100ea6d883d7 2024-09-09T19:26:30+00:00 TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA Cáris Maroni NUNES Regina Nardini TUNDISI Marcos Bryan HEINEMANN Saemi OGASSAWARA Leonardo José RICHTZENHAIN 1999-03-01T00:00:00Z https://doaj.org/article/21cb720a4144465da58f100ea6d883d7 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651999000200006&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/21cb720a4144465da58f100ea6d883d7 Revista do Instituto de Medicina Tropical de São Paulo, Vol 41, Iss 2, Pp 95-100 (1999) Toxocariasis Visceral larva migrans Toxocara canis Indirect antibody competition ELISA Immunodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1999 ftdoajarticles 2024-08-05T17:49:31Z Toxocariasis is caused by infection of man by Toxocara canis and Toxocara cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody, in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%). Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Toxocariasis Visceral larva migrans Toxocara canis Indirect antibody competition ELISA Immunodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Toxocariasis Visceral larva migrans Toxocara canis Indirect antibody competition ELISA Immunodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Cáris Maroni NUNES Regina Nardini TUNDISI Marcos Bryan HEINEMANN Saemi OGASSAWARA Leonardo José RICHTZENHAIN TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA |
topic_facet |
Toxocariasis Visceral larva migrans Toxocara canis Indirect antibody competition ELISA Immunodiagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Toxocariasis is caused by infection of man by Toxocara canis and Toxocara cati larvae, the common roundworm of dogs and cats. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. Non specific reactions are observed mainly due to cross-reactivity with Ascaris sp antigens. This investigation aimed at developing and evaluating an indirect antibody competition ELISA (IACE) employing a specific rabbit IgG anti-Toxocara canis excretory-secretory antigens as the competition antibody, in order to improve indirect ELISA specificity performed for toxocariasis diagnosis. For that, the rabbit IgG was previously absorbed by Ascaris suum adult antigens. Sensitivity and specificity of IACE were first evaluated in 28 serum samples of mice experimentally infected with T. canis embryonated eggs. Adopting cut-off value established in this population before infection, sensitivity and specificity were 100% after 20 days post-inoculation. For human population IACE was evaluated using sera from 440 patients with clinical signs of toxocariasis and the cut-off value was established with 60 serum samples from apparently healthy individuals. Using as reference test the indirect ELISA performed by Adolfo Lutz Institute, sensitivity was 60.2%, specificity was 98% and concordance was 77.3%. Repeatability of IACE was evaluated by the inter-reactions variation coefficient (2.4%). |
format |
Article in Journal/Newspaper |
author |
Cáris Maroni NUNES Regina Nardini TUNDISI Marcos Bryan HEINEMANN Saemi OGASSAWARA Leonardo José RICHTZENHAIN |
author_facet |
Cáris Maroni NUNES Regina Nardini TUNDISI Marcos Bryan HEINEMANN Saemi OGASSAWARA Leonardo José RICHTZENHAIN |
author_sort |
Cáris Maroni NUNES |
title |
TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA |
title_short |
TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA |
title_full |
TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA |
title_fullStr |
TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA |
title_full_unstemmed |
TOXOCARIASIS: SEROLOGICAL DIAGNOSIS BY INDIRECT ANTIBODY COMPETITION ELISA |
title_sort |
toxocariasis: serological diagnosis by indirect antibody competition elisa |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1999 |
url |
https://doaj.org/article/21cb720a4144465da58f100ea6d883d7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 41, Iss 2, Pp 95-100 (1999) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651999000200006&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/21cb720a4144465da58f100ea6d883d7 |
_version_ |
1809896094777212928 |