Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling
Objective: To investigate the effect of honokiol on oxidative damage in HaCaT human keratinocytes. Methods: HaCaT cells were exposed to hydrogen peroxide (H2O2), following pretreatment with various concentrations of honokiol. The alleviating effects of honokiol on HaCaT cell viability and cell death...
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Wolters Kluwer Medknow Publications
2021
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ftdoajarticles:oai:doaj.org/article:21ba0a41b2a54bb1a9761cc200f49be2 2023-05-15T15:08:48+02:00 Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling Yung Hyun Choi 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.311770 https://doaj.org/article/21ba0a41b2a54bb1a9761cc200f49be2 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=5;spage=222;epage=230;aulast=Choi https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.311770 https://doaj.org/article/21ba0a41b2a54bb1a9761cc200f49be2 Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 5, Pp 222-230 (2021) honokiol ros dna damage apoptosis ampk 1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.311770 2022-12-30T20:39:28Z Objective: To investigate the effect of honokiol on oxidative damage in HaCaT human keratinocytes. Methods: HaCaT cells were exposed to hydrogen peroxide (H2O2), following pretreatment with various concentrations of honokiol. The alleviating effects of honokiol on HaCaT cell viability and cell death, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of adenosine triphoaphate production against H2O2 were investigated. Western blotting analysis was used to analyze the expression levels of specific proteins. Results: Honokiol suppressed H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS accumulation. Honokiol also prevented apoptosis by inhibiting loss of mitochondrial membrane potential and release of cytochrome c from the mitochondria into the cytosol, decreasing the Bax/Bcl-2 ratio, and reducing the activity of caspase-3 in H2O2-stimulated HaCaT cells. In addition, honokiol attenuated H2O2-induced reduction of adenosine triphosphate content, and activation of AMP-activated protein kinase (AMPK) was markedly promoted by honokiol in H2O2-stimulated cells. Importantly, the anti-apoptosis and anti-proliferative activity of honokiol against H2O2 was further enhanced by adding an activator of AMPK, indicating that honokiol activated AMPK in HaCaT keratinocytes to protect against oxidative damage. Conclusions: The present results indicate that honokiol may be useful as a potential therapeutic agent against various oxidative stress-related skin diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 5 222 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
honokiol ros dna damage apoptosis ampk 1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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honokiol ros dna damage apoptosis ampk 1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Yung Hyun Choi Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling |
topic_facet |
honokiol ros dna damage apoptosis ampk 1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effect of honokiol on oxidative damage in HaCaT human keratinocytes. Methods: HaCaT cells were exposed to hydrogen peroxide (H2O2), following pretreatment with various concentrations of honokiol. The alleviating effects of honokiol on HaCaT cell viability and cell death, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of adenosine triphoaphate production against H2O2 were investigated. Western blotting analysis was used to analyze the expression levels of specific proteins. Results: Honokiol suppressed H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS accumulation. Honokiol also prevented apoptosis by inhibiting loss of mitochondrial membrane potential and release of cytochrome c from the mitochondria into the cytosol, decreasing the Bax/Bcl-2 ratio, and reducing the activity of caspase-3 in H2O2-stimulated HaCaT cells. In addition, honokiol attenuated H2O2-induced reduction of adenosine triphosphate content, and activation of AMP-activated protein kinase (AMPK) was markedly promoted by honokiol in H2O2-stimulated cells. Importantly, the anti-apoptosis and anti-proliferative activity of honokiol against H2O2 was further enhanced by adding an activator of AMPK, indicating that honokiol activated AMPK in HaCaT keratinocytes to protect against oxidative damage. Conclusions: The present results indicate that honokiol may be useful as a potential therapeutic agent against various oxidative stress-related skin diseases. |
format |
Article in Journal/Newspaper |
author |
Yung Hyun Choi |
author_facet |
Yung Hyun Choi |
author_sort |
Yung Hyun Choi |
title |
Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling |
title_short |
Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling |
title_full |
Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling |
title_fullStr |
Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling |
title_full_unstemmed |
Honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating AMPK signaling |
title_sort |
honokiol attenuates oxidative stress-induced cytotoxicity in human keratinocytes via activating ampk signaling |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.311770 https://doaj.org/article/21ba0a41b2a54bb1a9761cc200f49be2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 5, Pp 222-230 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=5;spage=222;epage=230;aulast=Choi https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.311770 https://doaj.org/article/21ba0a41b2a54bb1a9761cc200f49be2 |
op_doi |
https://doi.org/10.4103/2221-1691.311770 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
11 |
container_issue |
5 |
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222 |
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1766340097996750848 |