Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria
Abstract Background Major drawbacks of the available treatment against Chagas disease (American trypanosomiasis) include its toxicity and therapeutic inefficiency in the chronic phase of the infection, which makes it a concern among neglected diseases. Therefore, the discovery of alternative drugs f...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:217baf611d2145858a80082097335587 2023-05-15T15:14:15+02:00 Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria Tatiana Rodrigues Alexandre Marta Lopes Lima Mariana Kolos Galuppo Juliana Tonini Mesquita Matilia Ana do Nascimento Augusto Leonardo dos Santos Patricia Sartorelli Daniel Carvalho Pimenta Andre Gustavo Tempone 2017-05-01T00:00:00Z https://doi.org/10.1186/s40409-017-0120-0 https://doaj.org/article/217baf611d2145858a80082097335587 EN eng SciELO http://link.springer.com/article/10.1186/s40409-017-0120-0 https://doaj.org/toc/1678-9199 doi:10.1186/s40409-017-0120-0 1678-9199 https://doaj.org/article/217baf611d2145858a80082097335587 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 23, Iss 1, Pp 1-10 (2017) Pleurotus salmoneostramineus Ergosterol Trypanosoma cruzi Mechanism of action Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2017 ftdoajarticles https://doi.org/10.1186/s40409-017-0120-0 2022-12-31T00:59:51Z Abstract Background Major drawbacks of the available treatment against Chagas disease (American trypanosomiasis) include its toxicity and therapeutic inefficiency in the chronic phase of the infection, which makes it a concern among neglected diseases. Therefore, the discovery of alternative drugs for treating chronic Chagas disease requires immediate action. In this work, we evaluated the mushroom Pleurotus salmoneostramineus in the search for potential antiparasitic compounds. Methods Fruit bodies of the basidiomycete Pleurotus salmoneostramineus were triturated and submitted to organic solvent extraction. After liquid-liquid partition of the crude extract, three fractions were obtained and the bioguided fractionation study was conducted to isolate the active metabolites. The elucidation of the chemical structure was performed using GC-MS and NMR techniques. The biological assays for antiparasitic activity were carried out using trypomastigotes of Trypanosoma cruzi and murine macrophages for mammalian cytotoxicity. The mechanism of action of the isolated compound used different fluorescent probes to evaluate the plasma membrane permeability, the potential of the mitochondrial membrane and the intracellular levels of reactive oxygen species (ROS). Results The most abundant fraction showing the antiparasitic activity was isolated and chemically elucidated, confirming the presence of ergosterol. It showed anti-Trypanosoma cruzi activity against trypomastigotes, with an IC50 value of 51.3 μg/mL. The compound demonstrated no cytotoxicity against mammalian cells to the maximal tested concentration of 200 μg/mL. The mechanism of action of ergosterol in Trypanosoma cruzi trypomastigotes resulted in permeabilization of the plasma membrane, as well as depolarization of mitochondrial membrane potential, leading to parasite death. Nevertheless, no increase in ROS levels could be observed, suggesting damages to plasma membrane rather than an induction of oxidative stress in the parasite. Conclusions The selection of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 23 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pleurotus salmoneostramineus Ergosterol Trypanosoma cruzi Mechanism of action Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Pleurotus salmoneostramineus Ergosterol Trypanosoma cruzi Mechanism of action Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Tatiana Rodrigues Alexandre Marta Lopes Lima Mariana Kolos Galuppo Juliana Tonini Mesquita Matilia Ana do Nascimento Augusto Leonardo dos Santos Patricia Sartorelli Daniel Carvalho Pimenta Andre Gustavo Tempone Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria |
topic_facet |
Pleurotus salmoneostramineus Ergosterol Trypanosoma cruzi Mechanism of action Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Major drawbacks of the available treatment against Chagas disease (American trypanosomiasis) include its toxicity and therapeutic inefficiency in the chronic phase of the infection, which makes it a concern among neglected diseases. Therefore, the discovery of alternative drugs for treating chronic Chagas disease requires immediate action. In this work, we evaluated the mushroom Pleurotus salmoneostramineus in the search for potential antiparasitic compounds. Methods Fruit bodies of the basidiomycete Pleurotus salmoneostramineus were triturated and submitted to organic solvent extraction. After liquid-liquid partition of the crude extract, three fractions were obtained and the bioguided fractionation study was conducted to isolate the active metabolites. The elucidation of the chemical structure was performed using GC-MS and NMR techniques. The biological assays for antiparasitic activity were carried out using trypomastigotes of Trypanosoma cruzi and murine macrophages for mammalian cytotoxicity. The mechanism of action of the isolated compound used different fluorescent probes to evaluate the plasma membrane permeability, the potential of the mitochondrial membrane and the intracellular levels of reactive oxygen species (ROS). Results The most abundant fraction showing the antiparasitic activity was isolated and chemically elucidated, confirming the presence of ergosterol. It showed anti-Trypanosoma cruzi activity against trypomastigotes, with an IC50 value of 51.3 μg/mL. The compound demonstrated no cytotoxicity against mammalian cells to the maximal tested concentration of 200 μg/mL. The mechanism of action of ergosterol in Trypanosoma cruzi trypomastigotes resulted in permeabilization of the plasma membrane, as well as depolarization of mitochondrial membrane potential, leading to parasite death. Nevertheless, no increase in ROS levels could be observed, suggesting damages to plasma membrane rather than an induction of oxidative stress in the parasite. Conclusions The selection of ... |
format |
Article in Journal/Newspaper |
author |
Tatiana Rodrigues Alexandre Marta Lopes Lima Mariana Kolos Galuppo Juliana Tonini Mesquita Matilia Ana do Nascimento Augusto Leonardo dos Santos Patricia Sartorelli Daniel Carvalho Pimenta Andre Gustavo Tempone |
author_facet |
Tatiana Rodrigues Alexandre Marta Lopes Lima Mariana Kolos Galuppo Juliana Tonini Mesquita Matilia Ana do Nascimento Augusto Leonardo dos Santos Patricia Sartorelli Daniel Carvalho Pimenta Andre Gustavo Tempone |
author_sort |
Tatiana Rodrigues Alexandre |
title |
Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria |
title_short |
Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria |
title_full |
Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria |
title_fullStr |
Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria |
title_full_unstemmed |
Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria |
title_sort |
ergosterol isolated from the basidiomycete pleurotus salmoneostramineus affects trypanosoma cruzi plasma membrane and mitochondria |
publisher |
SciELO |
publishDate |
2017 |
url |
https://doi.org/10.1186/s40409-017-0120-0 https://doaj.org/article/217baf611d2145858a80082097335587 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 23, Iss 1, Pp 1-10 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s40409-017-0120-0 https://doaj.org/toc/1678-9199 doi:10.1186/s40409-017-0120-0 1678-9199 https://doaj.org/article/217baf611d2145858a80082097335587 |
op_doi |
https://doi.org/10.1186/s40409-017-0120-0 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
23 |
container_issue |
1 |
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1766344725594374144 |