The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus
Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral path...
Published in: | Memórias do Instituto Oswaldo Cruz |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2012
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0074-02762012000100002 https://doaj.org/article/217921f15740417cb971ee9c577109e2 |
id |
ftdoajarticles:oai:doaj.org/article:217921f15740417cb971ee9c577109e2 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:217921f15740417cb971ee9c577109e2 2023-10-01T03:55:33+02:00 The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus Adriana de Abreu Corrêa Caroline Rigotto Vanessa Moresco Cristian Rafael Kleemann Adriano Luiz Teixeira Carlos Rogério Poli Cláudia Maria Oliveira Simões Célia Regina Monte Barardi 2012-02-01T00:00:00Z https://doi.org/10.1590/S0074-02762012000100002 https://doaj.org/article/217921f15740417cb971ee9c577109e2 EN eng Fundação Oswaldo Cruz (FIOCRUZ) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000100002&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/S0074-02762012000100002 https://doaj.org/article/217921f15740417cb971ee9c577109e2 Memorias do Instituto Oswaldo Cruz, Vol 107, Iss 1, Pp 11-17 (2012) aquaculture depuration food safety shellfish viruses Microbiology QR1-502 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1590/S0074-02762012000100002 2023-09-03T00:38:12Z Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were contaminated with hepatitis A virus (HAV) or human adenovirus type 5 (HAdV5). After viral infection, the oysters were placed into a depuration tank and harvested after 48, 72 and 96 h. After sampling, various oyster tissues were dissected and homogenised and the viruses were eluted with alkaline conditions and precipitated with polyethylene glycol. The oyster samples were evaluated by cell culture methods, as well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover, at the end of the depuration period, the disinfected seawater was collected and analysed by PCR. The molecular assays showed that the HAdV5 genome was present in all of the depuration time samples, while the HAV genome was undetectable after 72 h of depuration. However, viral viability tests (integrated cell culture-PCR and immunofluorescence assay) indicated that both viruses were inactivated with 96 h of seawater recirculation. In conclusion, after 96 h of UV treatment, the depuration system studied in this work purified oysters that were artificially contaminated with HAdV5 and HAV. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Memórias do Instituto Oswaldo Cruz 107 1 11 17 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
aquaculture depuration food safety shellfish viruses Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
spellingShingle |
aquaculture depuration food safety shellfish viruses Microbiology QR1-502 Infectious and parasitic diseases RC109-216 Adriana de Abreu Corrêa Caroline Rigotto Vanessa Moresco Cristian Rafael Kleemann Adriano Luiz Teixeira Carlos Rogério Poli Cláudia Maria Oliveira Simões Célia Regina Monte Barardi The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
topic_facet |
aquaculture depuration food safety shellfish viruses Microbiology QR1-502 Infectious and parasitic diseases RC109-216 |
description |
Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were contaminated with hepatitis A virus (HAV) or human adenovirus type 5 (HAdV5). After viral infection, the oysters were placed into a depuration tank and harvested after 48, 72 and 96 h. After sampling, various oyster tissues were dissected and homogenised and the viruses were eluted with alkaline conditions and precipitated with polyethylene glycol. The oyster samples were evaluated by cell culture methods, as well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover, at the end of the depuration period, the disinfected seawater was collected and analysed by PCR. The molecular assays showed that the HAdV5 genome was present in all of the depuration time samples, while the HAV genome was undetectable after 72 h of depuration. However, viral viability tests (integrated cell culture-PCR and immunofluorescence assay) indicated that both viruses were inactivated with 96 h of seawater recirculation. In conclusion, after 96 h of UV treatment, the depuration system studied in this work purified oysters that were artificially contaminated with HAdV5 and HAV. |
format |
Article in Journal/Newspaper |
author |
Adriana de Abreu Corrêa Caroline Rigotto Vanessa Moresco Cristian Rafael Kleemann Adriano Luiz Teixeira Carlos Rogério Poli Cláudia Maria Oliveira Simões Célia Regina Monte Barardi |
author_facet |
Adriana de Abreu Corrêa Caroline Rigotto Vanessa Moresco Cristian Rafael Kleemann Adriano Luiz Teixeira Carlos Rogério Poli Cláudia Maria Oliveira Simões Célia Regina Monte Barardi |
author_sort |
Adriana de Abreu Corrêa |
title |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_short |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_full |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_fullStr |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_full_unstemmed |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_sort |
depuration dynamics of oysters (crassostrea gigas) artificially contaminated with hepatitis a virus and human adenovirus |
publisher |
Fundação Oswaldo Cruz (FIOCRUZ) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0074-02762012000100002 https://doaj.org/article/217921f15740417cb971ee9c577109e2 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Memorias do Instituto Oswaldo Cruz, Vol 107, Iss 1, Pp 11-17 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000100002&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/S0074-02762012000100002 https://doaj.org/article/217921f15740417cb971ee9c577109e2 |
op_doi |
https://doi.org/10.1590/S0074-02762012000100002 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
107 |
container_issue |
1 |
container_start_page |
11 |
op_container_end_page |
17 |
_version_ |
1778524117988802560 |