Cross-species infection potential of avian influenza H13 viruses isolated from wild aquatic birds to poultry and mammals

ABSTRACTWild aquatic birds are the primary hosts of H13 avian influenza viruses (AIVs). Herein, we performed a genetic analysis of two H13 AIVs isolated from wild birds in China and evaluated their infection potential in poultry to further explore the potential for transmission from wild aquatic bir...

Full description

Bibliographic Details
Published in:Emerging Microbes & Infections
Main Authors: Weiyang Sun, Menglin Zhao, Zhijun Yu, Yuanguo Li, Xinghai Zhang, Na Feng, Tiecheng Wang, Hongmei Wang, Hongbin He, Yongkun Zhao, Songtao Yang, Xianzhu Xia, Yuwei Gao
Format: Article in Journal/Newspaper
Language:English
Published: Taylor & Francis Group 2023
Subjects:
Avian influenza virus
H13
wild aquatic birds
poultry
mice
Infectious and parasitic diseases
RC109-216
Microbiology
QR1-502
Darby
Eurasian Curlew
Online Access:https://doi.org/10.1080/22221751.2023.2184177
https://doaj.org/article/211ffdda4bfd43bba56c2ac4cfcd4e6a
Description
Summary:ABSTRACTWild aquatic birds are the primary hosts of H13 avian influenza viruses (AIVs). Herein, we performed a genetic analysis of two H13 AIVs isolated from wild birds in China and evaluated their infection potential in poultry to further explore the potential for transmission from wild aquatic birds to poultry. Our results showed that the two strains belong to different groups, one strain (A/mallard/Dalian/DZ-137/2013; abbreviated as DZ137) belongs to Group I, whereas the other strain (A/Eurasian Curlew/Liaoning/ZH-385/2014; abbreviated as ZH385) belongs to Group III. In vitro experiments showed that both DZ137 and ZH385 can replicate efficiently in chicken embryo fibroblast cells. We found that these H13 AIVs can also efficiently replicate in mammalian cell lines, including human embryonic kidney cells and Madin-Darby canine kidney cells. In vivo experiments showed that DZ137 and ZH385 can infect 1-day-old specific pathogen-free (SPF) chickens, and that ZH385 has a higher replication ability in chickens than DZ137. Notably, only ZH385 can replicate efficiently in 10-day-old SPF chickens. However, neither DZ137 nor ZH385 can replicate well in turkeys and quails. Both DZ137 and ZH385 can replicate in 3-week-old mice. Serological surveillance of poultry showed a 4.6%-10.4% (15/328-34/328) antibody-positive rate against H13 AIVs in farm chickens. Our findings indicate that H13 AIVs have the replication ability in chickens and mice and may have a risk of crossing the host barrier from wild aquatic birds to poultry or mammals in the future.

Similar Items