The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting
Abstract: INTRODUCTION: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbap...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
|
Subjects: | |
Online Access: | https://doi.org/10.1590/0037-8682-0002-2016 https://doaj.org/article/210d71ce00ac4359a34b223be779c57e |
id |
ftdoajarticles:oai:doaj.org/article:210d71ce00ac4359a34b223be779c57e |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:210d71ce00ac4359a34b223be779c57e 2023-05-15T15:16:45+02:00 The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting Vanessa Cristine Kobs Jéssica Augustini Ferreira Thaís Alexandra Bobrowicz Leslie Ecker Ferreira Roseneide Campos Deglmann Glauco Adrieno Westphal Paulo Henrique Condeixa de França https://doi.org/10.1590/0037-8682-0002-2016 https://doaj.org/article/210d71ce00ac4359a34b223be779c57e EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000400433&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0002-2016 https://doaj.org/article/210d71ce00ac4359a34b223be779c57e Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 4, Pp 433-440 Acinetobacter calcoaceticus-Acinetobacter baumannii complex bla OXA ISAba1 Carbapenem antibiotics Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0002-2016 2022-12-31T03:06:27Z Abstract: INTRODUCTION: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. METHODS: Isolates identified over 6 consecutive years in a general hospital in Joinville, Southern Brazil, were evaluated. The investigation of 5 families of genes encoding oxacillinases and the ISAba1 sequence location relative to bla OXA genes was conducted using polymerase chain reaction. RESULTS: All isolates presented the bla OXA-51-like gene (n = 78), and 91% tested positive for the bla OXA-23-like gene (n = 71). The presence of ISAba1 was exclusively detected in isolates carrying the bla OXA-23-like gene. All isolates in which ISAba1 was found upstream of the bla OXA-23-like gene (n = 69) showed resistance to carbapenems, whereas the only isolate in which ISAba1 was not located near the bla OXA-23-like gene was susceptible to carbapenems. The ISAba1 sequence position of another bla OXA-23-like-positive isolate was inconclusive. The isolates exclusively carrying the bla OXA-51-like gene (n = 7) showed susceptibility to carbapenems. CONCLUSIONS: The presence of the ISAba1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Joinville ENVELOPE(-55.867,-55.867,-63.250,-63.250) Revista da Sociedade Brasileira de Medicina Tropical 49 4 433 440 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Acinetobacter calcoaceticus-Acinetobacter baumannii complex bla OXA ISAba1 Carbapenem antibiotics Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Acinetobacter calcoaceticus-Acinetobacter baumannii complex bla OXA ISAba1 Carbapenem antibiotics Arctic medicine. Tropical medicine RC955-962 Vanessa Cristine Kobs Jéssica Augustini Ferreira Thaís Alexandra Bobrowicz Leslie Ecker Ferreira Roseneide Campos Deglmann Glauco Adrieno Westphal Paulo Henrique Condeixa de França The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting |
topic_facet |
Acinetobacter calcoaceticus-Acinetobacter baumannii complex bla OXA ISAba1 Carbapenem antibiotics Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract: INTRODUCTION: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. METHODS: Isolates identified over 6 consecutive years in a general hospital in Joinville, Southern Brazil, were evaluated. The investigation of 5 families of genes encoding oxacillinases and the ISAba1 sequence location relative to bla OXA genes was conducted using polymerase chain reaction. RESULTS: All isolates presented the bla OXA-51-like gene (n = 78), and 91% tested positive for the bla OXA-23-like gene (n = 71). The presence of ISAba1 was exclusively detected in isolates carrying the bla OXA-23-like gene. All isolates in which ISAba1 was found upstream of the bla OXA-23-like gene (n = 69) showed resistance to carbapenems, whereas the only isolate in which ISAba1 was not located near the bla OXA-23-like gene was susceptible to carbapenems. The ISAba1 sequence position of another bla OXA-23-like-positive isolate was inconclusive. The isolates exclusively carrying the bla OXA-51-like gene (n = 7) showed susceptibility to carbapenems. CONCLUSIONS: The presence of the ISAba1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied. |
format |
Article in Journal/Newspaper |
author |
Vanessa Cristine Kobs Jéssica Augustini Ferreira Thaís Alexandra Bobrowicz Leslie Ecker Ferreira Roseneide Campos Deglmann Glauco Adrieno Westphal Paulo Henrique Condeixa de França |
author_facet |
Vanessa Cristine Kobs Jéssica Augustini Ferreira Thaís Alexandra Bobrowicz Leslie Ecker Ferreira Roseneide Campos Deglmann Glauco Adrieno Westphal Paulo Henrique Condeixa de França |
author_sort |
Vanessa Cristine Kobs |
title |
The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting |
title_short |
The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting |
title_full |
The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting |
title_fullStr |
The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting |
title_full_unstemmed |
The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting |
title_sort |
role of the genetic elements bla oxa and is aba 1 in the acinetobacter calcoaceticus-acinetobacter baumannii complex in carbapenem resistance in the hospital setting |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0002-2016 https://doaj.org/article/210d71ce00ac4359a34b223be779c57e |
long_lat |
ENVELOPE(-55.867,-55.867,-63.250,-63.250) |
geographic |
Arctic Joinville |
geographic_facet |
Arctic Joinville |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 4, Pp 433-440 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000400433&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0002-2016 https://doaj.org/article/210d71ce00ac4359a34b223be779c57e |
op_doi |
https://doi.org/10.1590/0037-8682-0002-2016 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
49 |
container_issue |
4 |
container_start_page |
433 |
op_container_end_page |
440 |
_version_ |
1766347052044779520 |