Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves
Melhania zavattarii Cufod is an endemic plant species to Ethiopia and is used to treat ailments related to kidney infection. The phytochemical composition and biological activity of M. zavattarii have been not reported yet. Therefore, the present work aimed to investigate phytochemical constituents...
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2023
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ftdoajarticles:oai:doaj.org/article:20ec94110aea48dabd26c2816b10f925 2023-07-02T03:31:33+02:00 Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves Teshome Gonfa Ayalew Temesgen Olyad Erba Ephriem T. Mengesha Muthusaravanan Sivasubramanian 2023-01-01T00:00:00Z https://doi.org/10.1155/2023/8820543 https://doaj.org/article/20ec94110aea48dabd26c2816b10f925 EN eng Hindawi Limited http://dx.doi.org/10.1155/2023/8820543 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/8820543 https://doaj.org/article/20ec94110aea48dabd26c2816b10f925 Journal of Tropical Medicine, Vol 2023 (2023) Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1155/2023/8820543 2023-06-11T00:34:53Z Melhania zavattarii Cufod is an endemic plant species to Ethiopia and is used to treat ailments related to kidney infection. The phytochemical composition and biological activity of M. zavattarii have been not reported yet. Therefore, the present work aimed to investigate phytochemical constituents and evaluate the antibacterial activity of different solvents’ leaf extracts and analyze the molecular binding capacity of isolated compounds from the chloroform leaf extract of M. zavattarii. Accordingly, preliminary phytochemical screening was tested by using standard procedures and the result indicated that phytosterols and terpenoids as major and others like alkaloids, saponins, flavonoids, tannins, phlobatannin, and coumarins were detected as minor in extracts. Antibacterial activity of the extracts was evaluated using the disk diffusion agar method, and the activities revealed that chloroform extract showed the highest inhibition zones, 12.08 ± 0.38, 14.00 ± 0.50, and 15.58 ± 0.63 mm against Escherichia coli at 50, 75, and 125 mg/mL concentrations, respectively, compared to that of n-hexane and methanol extracts at respective concentrations. Methanol extract showed the highest zone of inhibition 16.42 + 0.52 against Staphylococcus aureus at 125 mg/mL concentration compared to that of n-hexane and chloroform extracts. Two compounds, namely, β-amyrin palmitate (1) and lutein (2) were isolated and identified for the first time from the chloroform leaf extract of M. zavattarii, and structural elucidations of these compounds were accomplished by using spectroscopic methods (IR, UV, and NMR). For the molecular docking study, 1G2A, which is a protein of E. coli and chloramphenicol standard target, was selected. Binding energies of −9.09, −7.05, and −6.87 kcal/mol were calculated for β-amyrin palmitate, lutein, and chloramphenicol, respectively. The drug-likeness property result indicated that both β-amyrin palmitate and lutein violated two rules of Lipinski’s rule of five with molecular weight (g/mol) > 500 and LogP ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2023 1 12 |
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Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Teshome Gonfa Ayalew Temesgen Olyad Erba Ephriem T. Mengesha Muthusaravanan Sivasubramanian Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Melhania zavattarii Cufod is an endemic plant species to Ethiopia and is used to treat ailments related to kidney infection. The phytochemical composition and biological activity of M. zavattarii have been not reported yet. Therefore, the present work aimed to investigate phytochemical constituents and evaluate the antibacterial activity of different solvents’ leaf extracts and analyze the molecular binding capacity of isolated compounds from the chloroform leaf extract of M. zavattarii. Accordingly, preliminary phytochemical screening was tested by using standard procedures and the result indicated that phytosterols and terpenoids as major and others like alkaloids, saponins, flavonoids, tannins, phlobatannin, and coumarins were detected as minor in extracts. Antibacterial activity of the extracts was evaluated using the disk diffusion agar method, and the activities revealed that chloroform extract showed the highest inhibition zones, 12.08 ± 0.38, 14.00 ± 0.50, and 15.58 ± 0.63 mm against Escherichia coli at 50, 75, and 125 mg/mL concentrations, respectively, compared to that of n-hexane and methanol extracts at respective concentrations. Methanol extract showed the highest zone of inhibition 16.42 + 0.52 against Staphylococcus aureus at 125 mg/mL concentration compared to that of n-hexane and chloroform extracts. Two compounds, namely, β-amyrin palmitate (1) and lutein (2) were isolated and identified for the first time from the chloroform leaf extract of M. zavattarii, and structural elucidations of these compounds were accomplished by using spectroscopic methods (IR, UV, and NMR). For the molecular docking study, 1G2A, which is a protein of E. coli and chloramphenicol standard target, was selected. Binding energies of −9.09, −7.05, and −6.87 kcal/mol were calculated for β-amyrin palmitate, lutein, and chloramphenicol, respectively. The drug-likeness property result indicated that both β-amyrin palmitate and lutein violated two rules of Lipinski’s rule of five with molecular weight (g/mol) > 500 and LogP ... |
format |
Article in Journal/Newspaper |
author |
Teshome Gonfa Ayalew Temesgen Olyad Erba Ephriem T. Mengesha Muthusaravanan Sivasubramanian |
author_facet |
Teshome Gonfa Ayalew Temesgen Olyad Erba Ephriem T. Mengesha Muthusaravanan Sivasubramanian |
author_sort |
Teshome Gonfa |
title |
Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves |
title_short |
Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves |
title_full |
Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves |
title_fullStr |
Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves |
title_full_unstemmed |
Phytochemicals Analysis, In Vitro Antibacterial Activities of Extracts, and Molecular Docking Studies of the Isolated Compounds from Melhania zavattarii Cufod Leaves |
title_sort |
phytochemicals analysis, in vitro antibacterial activities of extracts, and molecular docking studies of the isolated compounds from melhania zavattarii cufod leaves |
publisher |
Hindawi Limited |
publishDate |
2023 |
url |
https://doi.org/10.1155/2023/8820543 https://doaj.org/article/20ec94110aea48dabd26c2816b10f925 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2023 (2023) |
op_relation |
http://dx.doi.org/10.1155/2023/8820543 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/8820543 https://doaj.org/article/20ec94110aea48dabd26c2816b10f925 |
op_doi |
https://doi.org/10.1155/2023/8820543 |
container_title |
Journal of Tropical Medicine |
container_volume |
2023 |
container_start_page |
1 |
op_container_end_page |
12 |
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1770270928124510208 |