Isolation and identification of compounds from Phaleria macrocarpa (Scheff.) Boerl fruit extract

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Emanuel Dani Ramdani, Ujiatmi Dwi Marlupi, James Sinambela, Raymond Rubianto Tjandrawinata
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Phaleria macrocarpa; Glyceryl pentacosanoate; 1,7-Dihydroxy-3,6-dimethoxyxanthone; 1,6,7-Trihydroxy-3-methoxyxanthone; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2016.12.018; https://doaj.org/article/20bf31a26d4d4877a79ff065a3277b74

Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa (P. macrocarpa) fruit ethanolic extract. Methods: Dried fruit of P. macrocarpa was extracted with 90% ethanol and partitioned between n-hexane/H2O and ethyl acetate/H2O. The organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance (NMR), carbon-13 NMR)], 2D-NMR (heteronuclear multiple-quantum correlation and heteronuclear multiple-bond correlation), and mass spectrum. Results: Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P. macrocarpa fruit resulted in isolation of nine compounds. Conclusions: A new compound was isolated and identified as glyceryl pentacosanoate. Also, two xanthones, which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are firstly reported to be isolated from P. macrocarpa.