Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates.
Background Melioidosis is an endemic disease in southeast Asia and northern Australia caused by the saprophytic bacteria Burkholderia pseudomallei, with a high mortality rate. The clinical presentation is multifaceted, with symptoms ranging from acute septicemia to multiple chronic abscesses. Here,...
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ftdoajarticles:oai:doaj.org/article:2084023be3f44dbb920362d7c855a217 2023-05-15T15:11:19+02:00 Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates. Marine Schnetterle Olivier Gorgé Flora Nolent Aïda Boughammoura Véronique Sarilar Cécile Vigier Sophie Guillier Lionel Koch Nicolas Degand Vincent Ramisse Xavier Tichadou Maria Girleanu Anne-Laure Favier Eric Valade Fabrice Biot Fabienne Neulat-Ripoll 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008913 https://doaj.org/article/2084023be3f44dbb920362d7c855a217 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008913 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008913 https://doaj.org/article/2084023be3f44dbb920362d7c855a217 PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0008913 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008913 2022-12-31T10:07:30Z Background Melioidosis is an endemic disease in southeast Asia and northern Australia caused by the saprophytic bacteria Burkholderia pseudomallei, with a high mortality rate. The clinical presentation is multifaceted, with symptoms ranging from acute septicemia to multiple chronic abscesses. Here, we report a chronic case of melioidosis in a patient who lived in Malaysia in the 70s and was suspected of contracting tuberculosis. Approximately 40 years later, in 2014, he was diagnosed with pauci-symptomatic melioidosis during a routine examination. Four strains were isolated from a single sample. They showed divergent morphotypes and divergent antibiotic susceptibility, with some strains showing resistance to trimethoprim-sulfamethoxazole and fluoroquinolones. In 2016, clinical samples were still positive for B. pseudomallei, and only one type of strain, showing atypical resistance to meropenem, was isolated. Principal findings We performed whole genome sequencing and RT-qPCR analysis on the strains isolated during this study to gain further insights into their differences. We thus identified two types of resistance mechanisms in these clinical strains. The first one was an adaptive and transient mechanism that disappeared during the course of laboratory sub-cultures; the second was a mutation in the efflux pump regulator amrR, associated with the overexpression of the related transporter. Conclusion The development of such mechanisms may have a clinical impact on antibiotic treatment. Indeed, their transient nature could lead to an undiagnosed resistance. Efflux overexpression due to mutation leads to an important multiple resistance, reducing the effectiveness of antibiotics during treatment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 2 e0008913 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Marine Schnetterle Olivier Gorgé Flora Nolent Aïda Boughammoura Véronique Sarilar Cécile Vigier Sophie Guillier Lionel Koch Nicolas Degand Vincent Ramisse Xavier Tichadou Maria Girleanu Anne-Laure Favier Eric Valade Fabrice Biot Fabienne Neulat-Ripoll Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Melioidosis is an endemic disease in southeast Asia and northern Australia caused by the saprophytic bacteria Burkholderia pseudomallei, with a high mortality rate. The clinical presentation is multifaceted, with symptoms ranging from acute septicemia to multiple chronic abscesses. Here, we report a chronic case of melioidosis in a patient who lived in Malaysia in the 70s and was suspected of contracting tuberculosis. Approximately 40 years later, in 2014, he was diagnosed with pauci-symptomatic melioidosis during a routine examination. Four strains were isolated from a single sample. They showed divergent morphotypes and divergent antibiotic susceptibility, with some strains showing resistance to trimethoprim-sulfamethoxazole and fluoroquinolones. In 2016, clinical samples were still positive for B. pseudomallei, and only one type of strain, showing atypical resistance to meropenem, was isolated. Principal findings We performed whole genome sequencing and RT-qPCR analysis on the strains isolated during this study to gain further insights into their differences. We thus identified two types of resistance mechanisms in these clinical strains. The first one was an adaptive and transient mechanism that disappeared during the course of laboratory sub-cultures; the second was a mutation in the efflux pump regulator amrR, associated with the overexpression of the related transporter. Conclusion The development of such mechanisms may have a clinical impact on antibiotic treatment. Indeed, their transient nature could lead to an undiagnosed resistance. Efflux overexpression due to mutation leads to an important multiple resistance, reducing the effectiveness of antibiotics during treatment. |
format |
Article in Journal/Newspaper |
author |
Marine Schnetterle Olivier Gorgé Flora Nolent Aïda Boughammoura Véronique Sarilar Cécile Vigier Sophie Guillier Lionel Koch Nicolas Degand Vincent Ramisse Xavier Tichadou Maria Girleanu Anne-Laure Favier Eric Valade Fabrice Biot Fabienne Neulat-Ripoll |
author_facet |
Marine Schnetterle Olivier Gorgé Flora Nolent Aïda Boughammoura Véronique Sarilar Cécile Vigier Sophie Guillier Lionel Koch Nicolas Degand Vincent Ramisse Xavier Tichadou Maria Girleanu Anne-Laure Favier Eric Valade Fabrice Biot Fabienne Neulat-Ripoll |
author_sort |
Marine Schnetterle |
title |
Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates. |
title_short |
Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates. |
title_full |
Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates. |
title_fullStr |
Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates. |
title_full_unstemmed |
Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates. |
title_sort |
genomic and rt-qpcr analysis of trimethoprim-sulfamethoxazole and meropenem resistance in burkholderia pseudomallei clinical isolates. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0008913 https://doaj.org/article/2084023be3f44dbb920362d7c855a217 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0008913 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008913 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008913 https://doaj.org/article/2084023be3f44dbb920362d7c855a217 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008913 |
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PLOS Neglected Tropical Diseases |
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2 |
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e0008913 |
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