Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation
The cryopreservation of sperm has become the subject of research for successful artificial insemination technologies. Antifreeze proteins (AFPs), one of the factors necessary for effective cryopreservation, are derived from certain Antarctic organisms. These proteins decrease the freezing point of w...
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The Korean Society of Animal Reproduction and Biotechnology
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ftdoajarticles:oai:doaj.org/article:2070c8c5fcb74fea986e6dd91d13ce89 2023-05-15T13:53:06+02:00 Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation Daeyoung Kim 2016-12-01T00:00:00Z https://doi.org/10.12750/JET.2016.31.4.355 https://doaj.org/article/2070c8c5fcb74fea986e6dd91d13ce89 EN KO eng kor The Korean Society of Animal Reproduction and Biotechnology http://www.e-jarb.org/journal/view.html?uid=1551&vmd=Full https://doaj.org/toc/2671-4639 https://doaj.org/toc/2671-4663 doi:10.12750/JET.2016.31.4.355 2671-4639 2671-4663 https://doaj.org/article/2070c8c5fcb74fea986e6dd91d13ce89 Journal of Animal Reproduction and Biotechnology, Vol 31, Iss 4, Pp 355-365 (2016) antifreeze protein miniature pig sperm cryopreservation dna integrity Biotechnology TP248.13-248.65 Medicine (General) R5-920 Internal medicine RC31-1245 article 2016 ftdoajarticles https://doi.org/10.12750/JET.2016.31.4.355 2022-12-31T08:15:51Z The cryopreservation of sperm has become the subject of research for successful artificial insemination technologies. Antifreeze proteins (AFPs), one of the factors necessary for effective cryopreservation, are derived from certain Antarctic organisms. These proteins decrease the freezing point of water within these organisms to below the temperature of the surrounding seawater to protect the organism from cold shock. Accordingly, a recent study found that AFPs can increase the motility and viability of spermatozoa during cryopreservation. To evaluate this relationship, we performed cryopreservation of boar sperm with AFPs produced in the Arctic yeast Leucosporidium sp. AFP expression system at four concentrations (0, 0.01, 0.1, and 1 μg/ml) and evaluated motility using computer assisted sperm analysis. DNA damage to boar spermatozoa was measured by the comet assay, and sperm membrane integrity and acrosome integrity were evaluated by flow cytometry. The results showed that motility was positively affected by the addition of AFP at each concentration except 1 μg/ml (p<0.001). Although cryopreservation with AFP decreased the viability of the boar sperm using, the tail DNA analyses showed that there was no significant difference between the control and the addition of 0.1 or 0.01 μg/ml AFP. In addition, the percentage of live sperm with intact acrosomes showed the least significant difference between the control and 0.1 μg/ml AFP (p<0.05), but increased with 1 μg/ml AFP (p<0.001). Our results indicate that the addition of AFP during boar sperm cryopreservation can improve viability and acrosome integrity after thawing. Article in Journal/Newspaper Antarc* Antarctic Arctic Directory of Open Access Journals: DOAJ Articles Arctic Antarctic Journal of Animal Reproduction and Biotechnology 31 4 355 365 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Korean |
topic |
antifreeze protein miniature pig sperm cryopreservation dna integrity Biotechnology TP248.13-248.65 Medicine (General) R5-920 Internal medicine RC31-1245 |
spellingShingle |
antifreeze protein miniature pig sperm cryopreservation dna integrity Biotechnology TP248.13-248.65 Medicine (General) R5-920 Internal medicine RC31-1245 Daeyoung Kim Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation |
topic_facet |
antifreeze protein miniature pig sperm cryopreservation dna integrity Biotechnology TP248.13-248.65 Medicine (General) R5-920 Internal medicine RC31-1245 |
description |
The cryopreservation of sperm has become the subject of research for successful artificial insemination technologies. Antifreeze proteins (AFPs), one of the factors necessary for effective cryopreservation, are derived from certain Antarctic organisms. These proteins decrease the freezing point of water within these organisms to below the temperature of the surrounding seawater to protect the organism from cold shock. Accordingly, a recent study found that AFPs can increase the motility and viability of spermatozoa during cryopreservation. To evaluate this relationship, we performed cryopreservation of boar sperm with AFPs produced in the Arctic yeast Leucosporidium sp. AFP expression system at four concentrations (0, 0.01, 0.1, and 1 μg/ml) and evaluated motility using computer assisted sperm analysis. DNA damage to boar spermatozoa was measured by the comet assay, and sperm membrane integrity and acrosome integrity were evaluated by flow cytometry. The results showed that motility was positively affected by the addition of AFP at each concentration except 1 μg/ml (p<0.001). Although cryopreservation with AFP decreased the viability of the boar sperm using, the tail DNA analyses showed that there was no significant difference between the control and the addition of 0.1 or 0.01 μg/ml AFP. In addition, the percentage of live sperm with intact acrosomes showed the least significant difference between the control and 0.1 μg/ml AFP (p<0.05), but increased with 1 μg/ml AFP (p<0.001). Our results indicate that the addition of AFP during boar sperm cryopreservation can improve viability and acrosome integrity after thawing. |
format |
Article in Journal/Newspaper |
author |
Daeyoung Kim |
author_facet |
Daeyoung Kim |
author_sort |
Daeyoung Kim |
title |
Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation |
title_short |
Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation |
title_full |
Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation |
title_fullStr |
Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation |
title_full_unstemmed |
Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation |
title_sort |
evaluation of antifreeze proteins on miniature pig sperm viability, dna damage, and acrosome status during cryopreservation |
publisher |
The Korean Society of Animal Reproduction and Biotechnology |
publishDate |
2016 |
url |
https://doi.org/10.12750/JET.2016.31.4.355 https://doaj.org/article/2070c8c5fcb74fea986e6dd91d13ce89 |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_source |
Journal of Animal Reproduction and Biotechnology, Vol 31, Iss 4, Pp 355-365 (2016) |
op_relation |
http://www.e-jarb.org/journal/view.html?uid=1551&vmd=Full https://doaj.org/toc/2671-4639 https://doaj.org/toc/2671-4663 doi:10.12750/JET.2016.31.4.355 2671-4639 2671-4663 https://doaj.org/article/2070c8c5fcb74fea986e6dd91d13ce89 |
op_doi |
https://doi.org/10.12750/JET.2016.31.4.355 |
container_title |
Journal of Animal Reproduction and Biotechnology |
container_volume |
31 |
container_issue |
4 |
container_start_page |
355 |
op_container_end_page |
365 |
_version_ |
1766258062009565184 |