Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis).
Leptospirosis is the most widespread zoonotic disease in the world. It is caused by pathogenic spirochetes of the genus Leptospira spp. and is maintained in nature through chronic renal infection of carrier animals. Rodents and other small mammals are the main reservoirs. Information on leptospirosi...
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ftdoajarticles:oai:doaj.org/article:20536d3af568461e9efb75daa547c245 2023-05-15T18:26:17+02:00 Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis). Sylvia Grune Loffler Virginia Rago Mara Martínez Marcela Uhart Monica Florin-Christensen Graciela Romero Bibiana Brihuega 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0144974 https://doaj.org/article/20536d3af568461e9efb75daa547c245 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4700976?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0144974 https://doaj.org/article/20536d3af568461e9efb75daa547c245 PLoS ONE, Vol 10, Iss 12, p e0144974 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0144974 2022-12-31T12:34:46Z Leptospirosis is the most widespread zoonotic disease in the world. It is caused by pathogenic spirochetes of the genus Leptospira spp. and is maintained in nature through chronic renal infection of carrier animals. Rodents and other small mammals are the main reservoirs. Information on leptospirosis in marine mammals is scarce; however, cases of leptospirosis have been documented in pinniped populations from the Pacific coast of North America from southern California to British Columbia. We report the isolation of a Leptospira spp. strain, here named Manara, from a kidney sample obtained from a Southern Right Whale (Eubalaena australis) calf, which stranded dead in Playa Manara, Península Valdés, Argentina. This strain showed motility and morphology typical of the genus Leptospira spp. under dark-field microscopy; and grew in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium and Fletcher medium after 90 days of incubation at 28°C. Considering the source of this bacterium, we tested its ability to grow in Fletcher medium diluted with seawater at different percentages (1%, 3%, 5%, 7% and 10% v/v). Bacterial growth was detected 48 h after inoculation of Fletcher medium supplemented with 5% sea water, demonstrating the halophilic nature of the strain Manara. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the radiation of the pathogenic species of the genus Leptospira spp., with sequence similarities within the range 97-100%, and closely related to L. interrogans. Two different PCR protocols targeting genus-specific pathogenic genes (G1-G2, B64I-B64II and LigB) gave positive results, which indicates that the strain Manara is likely pathogenic. Further studies are needed to confirm this possibility as well as determine its serogroup. These results could modify our understanding of the epidemiology of this zoonosis. Until now, the resistance and ability to grow in seawater for long periods of time had been proven for the strain Muggia of L. biflexa, a saprophytic species. To the ... Article in Journal/Newspaper Southern Right Whale Directory of Open Access Journals: DOAJ Articles Pacific Argentina PLOS ONE 10 12 e0144974 |
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Medicine R Science Q |
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Medicine R Science Q Sylvia Grune Loffler Virginia Rago Mara Martínez Marcela Uhart Monica Florin-Christensen Graciela Romero Bibiana Brihuega Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis). |
topic_facet |
Medicine R Science Q |
description |
Leptospirosis is the most widespread zoonotic disease in the world. It is caused by pathogenic spirochetes of the genus Leptospira spp. and is maintained in nature through chronic renal infection of carrier animals. Rodents and other small mammals are the main reservoirs. Information on leptospirosis in marine mammals is scarce; however, cases of leptospirosis have been documented in pinniped populations from the Pacific coast of North America from southern California to British Columbia. We report the isolation of a Leptospira spp. strain, here named Manara, from a kidney sample obtained from a Southern Right Whale (Eubalaena australis) calf, which stranded dead in Playa Manara, Península Valdés, Argentina. This strain showed motility and morphology typical of the genus Leptospira spp. under dark-field microscopy; and grew in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium and Fletcher medium after 90 days of incubation at 28°C. Considering the source of this bacterium, we tested its ability to grow in Fletcher medium diluted with seawater at different percentages (1%, 3%, 5%, 7% and 10% v/v). Bacterial growth was detected 48 h after inoculation of Fletcher medium supplemented with 5% sea water, demonstrating the halophilic nature of the strain Manara. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the radiation of the pathogenic species of the genus Leptospira spp., with sequence similarities within the range 97-100%, and closely related to L. interrogans. Two different PCR protocols targeting genus-specific pathogenic genes (G1-G2, B64I-B64II and LigB) gave positive results, which indicates that the strain Manara is likely pathogenic. Further studies are needed to confirm this possibility as well as determine its serogroup. These results could modify our understanding of the epidemiology of this zoonosis. Until now, the resistance and ability to grow in seawater for long periods of time had been proven for the strain Muggia of L. biflexa, a saprophytic species. To the ... |
format |
Article in Journal/Newspaper |
author |
Sylvia Grune Loffler Virginia Rago Mara Martínez Marcela Uhart Monica Florin-Christensen Graciela Romero Bibiana Brihuega |
author_facet |
Sylvia Grune Loffler Virginia Rago Mara Martínez Marcela Uhart Monica Florin-Christensen Graciela Romero Bibiana Brihuega |
author_sort |
Sylvia Grune Loffler |
title |
Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis). |
title_short |
Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis). |
title_full |
Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis). |
title_fullStr |
Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis). |
title_full_unstemmed |
Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis). |
title_sort |
isolation of a seawater tolerant leptospira spp. from a southern right whale (eubalaena australis). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0144974 https://doaj.org/article/20536d3af568461e9efb75daa547c245 |
geographic |
Pacific Argentina |
geographic_facet |
Pacific Argentina |
genre |
Southern Right Whale |
genre_facet |
Southern Right Whale |
op_source |
PLoS ONE, Vol 10, Iss 12, p e0144974 (2015) |
op_relation |
http://europepmc.org/articles/PMC4700976?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0144974 https://doaj.org/article/20536d3af568461e9efb75daa547c245 |
op_doi |
https://doi.org/10.1371/journal.pone.0144974 |
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PLOS ONE |
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10 |
container_issue |
12 |
container_start_page |
e0144974 |
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