Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins
Abstract Background Plasmodium sporozoites are the highly motile forms of malaria-causing parasites that are transmitted by the mosquito to the vertebrate host. Sporozoites need to enter and cross several cellular and tissue barriers for which they employ a set of surface proteins. Three of these pr...
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ftdoajarticles:oai:doaj.org/article:2050cfbe2e22467882b6624149238cf9 2023-05-15T15:08:59+02:00 Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins Konrad Beyer Simon Kracht Jessica Kehrer Mirko Singer Dennis Klug Friedrich Frischknecht 2021-10-01T00:00:00Z https://doi.org/10.1186/s12936-021-03960-3 https://doaj.org/article/2050cfbe2e22467882b6624149238cf9 EN eng BMC https://doi.org/10.1186/s12936-021-03960-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03960-3 1475-2875 https://doaj.org/article/2050cfbe2e22467882b6624149238cf9 Malaria Journal, Vol 20, Iss 1, Pp 1-12 (2021) Anopheles Transmission Migration Malaria Adhesion Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03960-3 2022-12-31T10:05:50Z Abstract Background Plasmodium sporozoites are the highly motile forms of malaria-causing parasites that are transmitted by the mosquito to the vertebrate host. Sporozoites need to enter and cross several cellular and tissue barriers for which they employ a set of surface proteins. Three of these proteins are members of the thrombospondin related anonymous protein (TRAP) family. Here, potential additive, synergistic or antagonistic roles of these adhesion proteins were investigated. Methods Four transgenic Plasmodium berghei parasite lines that lacked two or all three of the TRAP family adhesins TRAP, TLP and TREP were generated using positive–negative selection. The parasite lines were investigated for their capacity to attach to and move on glass, their ability to egress from oocysts and their capacity to enter mosquito salivary glands. One strain was in addition interrogated for its capacity to infect mice. Results The major phenotype of the TRAP single gene deletion dominates additional gene deletion phenotypes. All parasite lines including the one lacking all three proteins were able to conduct some form of active, if unproductive movement. Conclusions The individual TRAP-family adhesins appear to play functionally distinct roles during motility and infection. Other proteins must contribute to substrate adhesion and gliding motility. Graphical Abstract Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anopheles Transmission Migration Malaria Adhesion Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Anopheles Transmission Migration Malaria Adhesion Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Konrad Beyer Simon Kracht Jessica Kehrer Mirko Singer Dennis Klug Friedrich Frischknecht Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins |
topic_facet |
Anopheles Transmission Migration Malaria Adhesion Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium sporozoites are the highly motile forms of malaria-causing parasites that are transmitted by the mosquito to the vertebrate host. Sporozoites need to enter and cross several cellular and tissue barriers for which they employ a set of surface proteins. Three of these proteins are members of the thrombospondin related anonymous protein (TRAP) family. Here, potential additive, synergistic or antagonistic roles of these adhesion proteins were investigated. Methods Four transgenic Plasmodium berghei parasite lines that lacked two or all three of the TRAP family adhesins TRAP, TLP and TREP were generated using positive–negative selection. The parasite lines were investigated for their capacity to attach to and move on glass, their ability to egress from oocysts and their capacity to enter mosquito salivary glands. One strain was in addition interrogated for its capacity to infect mice. Results The major phenotype of the TRAP single gene deletion dominates additional gene deletion phenotypes. All parasite lines including the one lacking all three proteins were able to conduct some form of active, if unproductive movement. Conclusions The individual TRAP-family adhesins appear to play functionally distinct roles during motility and infection. Other proteins must contribute to substrate adhesion and gliding motility. Graphical Abstract |
format |
Article in Journal/Newspaper |
author |
Konrad Beyer Simon Kracht Jessica Kehrer Mirko Singer Dennis Klug Friedrich Frischknecht |
author_facet |
Konrad Beyer Simon Kracht Jessica Kehrer Mirko Singer Dennis Klug Friedrich Frischknecht |
author_sort |
Konrad Beyer |
title |
Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins |
title_short |
Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins |
title_full |
Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins |
title_fullStr |
Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins |
title_full_unstemmed |
Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins |
title_sort |
limited plasmodium sporozoite gliding motility in the absence of trap family adhesins |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03960-3 https://doaj.org/article/2050cfbe2e22467882b6624149238cf9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-12 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03960-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03960-3 1475-2875 https://doaj.org/article/2050cfbe2e22467882b6624149238cf9 |
op_doi |
https://doi.org/10.1186/s12936-021-03960-3 |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
_version_ |
1766340243419561984 |