Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures
Abstract Background In vitro cultivation of Plasmodium falciparum is usually carried out through the continuous preservation of infected erythrocytes deposited in static thin layers of settled haematocrit. This technique, called the candle-jar method, was first achieved by Trager and Jensen in 1976...
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ftdoajarticles:oai:doaj.org/article:2048b5a04d5e4f5f87b0b74d8604a7d5 2023-05-15T15:06:24+02:00 Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures López Daniel Herreros Esperanza A Valls Joaquim Vidal Jaume M Prats Clara Rosal Marina D Ferrer Jordi Gargallo Domingo 2008-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-203 https://doaj.org/article/2048b5a04d5e4f5f87b0b74d8604a7d5 EN eng BMC http://www.malariajournal.com/content/7/1/203 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-203 1475-2875 https://doaj.org/article/2048b5a04d5e4f5f87b0b74d8604a7d5 Malaria Journal, Vol 7, Iss 1, p 203 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-203 2022-12-30T21:41:17Z Abstract Background In vitro cultivation of Plasmodium falciparum is usually carried out through the continuous preservation of infected erythrocytes deposited in static thin layers of settled haematocrit. This technique, called the candle-jar method, was first achieved by Trager and Jensen in 1976 and has undergone slight modifications since then. However, no systematic studies concerning the geometry of the haematocrit layer have been carried out. In this work, a thorough investigation of the effects of the geometric culturing conditions on the parasite's development is presented. Methods Several experimental trials exploring different settings have been carried out, covering haematocrit layer depths that ranged from 6 mm to 3 mm and separation between the walls of the culturing device that ranged from 7.5 mm to 9 mm. The obtained results have been analysed and compared to different system-level models and to an Individual-Based Model. Conclusion In line with the results, a mechanism governing the propagation of the infection which limits it to the vicinity of the interface between the haematocrit layer and the culture medium is deduced, and the most appropriate configurations are proposed for further experimental assays. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 López Daniel Herreros Esperanza A Valls Joaquim Vidal Jaume M Prats Clara Rosal Marina D Ferrer Jordi Gargallo Domingo Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In vitro cultivation of Plasmodium falciparum is usually carried out through the continuous preservation of infected erythrocytes deposited in static thin layers of settled haematocrit. This technique, called the candle-jar method, was first achieved by Trager and Jensen in 1976 and has undergone slight modifications since then. However, no systematic studies concerning the geometry of the haematocrit layer have been carried out. In this work, a thorough investigation of the effects of the geometric culturing conditions on the parasite's development is presented. Methods Several experimental trials exploring different settings have been carried out, covering haematocrit layer depths that ranged from 6 mm to 3 mm and separation between the walls of the culturing device that ranged from 7.5 mm to 9 mm. The obtained results have been analysed and compared to different system-level models and to an Individual-Based Model. Conclusion In line with the results, a mechanism governing the propagation of the infection which limits it to the vicinity of the interface between the haematocrit layer and the culture medium is deduced, and the most appropriate configurations are proposed for further experimental assays. |
format |
Article in Journal/Newspaper |
author |
López Daniel Herreros Esperanza A Valls Joaquim Vidal Jaume M Prats Clara Rosal Marina D Ferrer Jordi Gargallo Domingo |
author_facet |
López Daniel Herreros Esperanza A Valls Joaquim Vidal Jaume M Prats Clara Rosal Marina D Ferrer Jordi Gargallo Domingo |
author_sort |
López Daniel |
title |
Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures |
title_short |
Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures |
title_full |
Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures |
title_fullStr |
Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures |
title_full_unstemmed |
Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures |
title_sort |
effect of the haematocrit layer geometry on plasmodium falciparum static thin-layer in vitro cultures |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-203 https://doaj.org/article/2048b5a04d5e4f5f87b0b74d8604a7d5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 203 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/203 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-203 1475-2875 https://doaj.org/article/2048b5a04d5e4f5f87b0b74d8604a7d5 |
op_doi |
https://doi.org/10.1186/1475-2875-7-203 |
container_title |
Malaria Journal |
container_volume |
7 |
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1 |
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1766338010957217792 |