Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings.
The road to malaria elimination for low- and middle-income countries is paved with obstacles, including the complexity and high costs of advanced molecular methods for genomic analysis. The usefulness of PCR-RFLP as less complex and affordable molecular surveillance tool in low-endemic malaria regio...
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ftdoajarticles:oai:doaj.org/article:202476d169654c488ba4151aafc98331 2024-01-14T10:05:00+01:00 Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. Mergiory Y Labadie-Bracho Malti R Adhin 2023-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011747 https://doaj.org/article/202476d169654c488ba4151aafc98331 EN eng Public Library of Science (PLoS) https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011747&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011747 https://doaj.org/article/202476d169654c488ba4151aafc98331 PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0011747 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011747 2023-12-17T01:44:39Z The road to malaria elimination for low- and middle-income countries is paved with obstacles, including the complexity and high costs of advanced molecular methods for genomic analysis. The usefulness of PCR-RFLP as less complex and affordable molecular surveillance tool in low-endemic malaria regions was assessed in a cross-sectional study conducted in Suriname, currently striving for malaria elimination, but plagued by recent P. vivax outbreaks. Molecular analysis of two highly polymorphic genes Pvmsp-1 F2 and Pvmsp-3α was performed for 49 samples, collected during October 2019 through September 2021 from four different regions with varying malaria transmission risks. RFLP-profiling revealed that outbreak samples from three indigenous villages, almost exclusively, harbored a single clonal type, matching the "Palumeu" lineage previously described in 2019, despite multiple relapses and drug pressure exerted by mass drug administration events, suggesting a limited P. vivax hypnozoite reservoir in Suriname. In contrast, isolates originating from Sophie, a mining area in neighboring French Guiana displayed a highly heterogeneous parasite population consistent with its endemic malaria status, demonstrating the differentiating capacity and thus the usefulness of PCR-RFLP for P. vivax genetic diversity studies. Outbreak reconstruction emphasized the impact of undetected human movement and relapses on reintroduction and resurgence of P. vivax malaria and PCR-RFLP monitoring of circulating parasites guided the roll-out of targeted interventions. PCR-RFLP seems a suitable molecular alternative in low-endemic areas with restricted resources for outbreak analysis, for monitoring the spread or containment of circulating strains and for identification of imported cases or potential foci. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 11 e0011747 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mergiory Y Labadie-Bracho Malti R Adhin Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The road to malaria elimination for low- and middle-income countries is paved with obstacles, including the complexity and high costs of advanced molecular methods for genomic analysis. The usefulness of PCR-RFLP as less complex and affordable molecular surveillance tool in low-endemic malaria regions was assessed in a cross-sectional study conducted in Suriname, currently striving for malaria elimination, but plagued by recent P. vivax outbreaks. Molecular analysis of two highly polymorphic genes Pvmsp-1 F2 and Pvmsp-3α was performed for 49 samples, collected during October 2019 through September 2021 from four different regions with varying malaria transmission risks. RFLP-profiling revealed that outbreak samples from three indigenous villages, almost exclusively, harbored a single clonal type, matching the "Palumeu" lineage previously described in 2019, despite multiple relapses and drug pressure exerted by mass drug administration events, suggesting a limited P. vivax hypnozoite reservoir in Suriname. In contrast, isolates originating from Sophie, a mining area in neighboring French Guiana displayed a highly heterogeneous parasite population consistent with its endemic malaria status, demonstrating the differentiating capacity and thus the usefulness of PCR-RFLP for P. vivax genetic diversity studies. Outbreak reconstruction emphasized the impact of undetected human movement and relapses on reintroduction and resurgence of P. vivax malaria and PCR-RFLP monitoring of circulating parasites guided the roll-out of targeted interventions. PCR-RFLP seems a suitable molecular alternative in low-endemic areas with restricted resources for outbreak analysis, for monitoring the spread or containment of circulating strains and for identification of imported cases or potential foci. |
format |
Article in Journal/Newspaper |
author |
Mergiory Y Labadie-Bracho Malti R Adhin |
author_facet |
Mergiory Y Labadie-Bracho Malti R Adhin |
author_sort |
Mergiory Y Labadie-Bracho |
title |
Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. |
title_short |
Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. |
title_full |
Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. |
title_fullStr |
Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. |
title_full_unstemmed |
Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. |
title_sort |
advocating for pcr-rflp as molecular tool within malaria programs in low endemic areas and low resource settings. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011747 https://doaj.org/article/202476d169654c488ba4151aafc98331 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0011747 (2023) |
op_relation |
https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011747&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011747 https://doaj.org/article/202476d169654c488ba4151aafc98331 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011747 |
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PLOS Neglected Tropical Diseases |
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17 |
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11 |
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