Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal
This study aimed to evaluate the antioxidant, antiarthritic, and anti-inflammatory properties of extracts from the leaves of twelve different medicinal plants in Nepal. We then evaluated the total phenolic, flavonoid, and tannin contents of the extract using in-vitro assays and characterized it usin...
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Hindawi Limited
2023
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ftdoajarticles:oai:doaj.org/article:201a036ffffe40f9a735e83ebfa95a71 2023-12-10T09:46:13+01:00 Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal Amit Kumar Shrivastava Muskan Keshari Manisha Neupane Sheshbhan Chaudhary Purna Kala Dhakal Laxmi Shrestha Anjan Palikhey Chandrajeet Kumar Yadav Gopal Lamichhane Mohammad Ujair Shekh Rakesh Kumar Yadav 2023-01-01T00:00:00Z https://doi.org/10.1155/2023/6641018 https://doaj.org/article/201a036ffffe40f9a735e83ebfa95a71 EN eng Hindawi Limited http://dx.doi.org/10.1155/2023/6641018 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/6641018 https://doaj.org/article/201a036ffffe40f9a735e83ebfa95a71 Journal of Tropical Medicine, Vol 2023 (2023) Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1155/2023/6641018 2023-11-12T01:35:29Z This study aimed to evaluate the antioxidant, antiarthritic, and anti-inflammatory properties of extracts from the leaves of twelve different medicinal plants in Nepal. We then evaluated the total phenolic, flavonoid, and tannin contents of the extract using in-vitro assays and characterized it using GC-MS analysis. Results revealed that most of the leaf extracts contained phenolic compounds, flavonoids, tannins, alkaloids, and saponins. Few plants also showed the presence of glycosides, phytate, and vitamin C. Among the studied plants, Neolamarckia cadamba exhibited the highest total phenolic and tannin contents, as 241.53 ± 0.20 µg of gallic acid equivalent/mg and 74.48 ± 1.081 µg of tannic acid equivalent/mg, respectively. Ipomoea batatas exhibited the highest total flavonoid content, as 53.051 ± 1.11 µg of quercetin equivalent/mg. Moreover, Raphanus sativus demonstrated significant ferrous ion chelating, 2,2-diphenyl-1-picrylhydrazyl, hydrogen peroxide scavenging, and total antioxidant activities with IC50 value of 4.76 ± 0.68 µg/mL, 5.84 ± 0.14 µg/mL, 6.89 ± 0.16 µg/mL, and 8.99 ± 0.20 µg/mL, respectively. Similarly, Colocasia esculenta and Cicer arietinum exhibited the highest hydroxyl radical and nitric oxide scavenging activities, measuring IC50 value of 7.22 ± 0.56 µg/mL and 9.06 ± 0.10 µg/mL, respectively. Among all the extracts, Amorphophallus paeoniifolius displayed significant human red blood cell (HRBC) membrane stabilization activity (IC50 = 6.22 ± 0.78 µg/mL). Furthermore, Raphanus sativus, Chenopodium album, Cicer arietinum, and Murraya koenigii exhibited the highest inhibitory activities against protein denaturation with bovine serum albumin, antiarthritic, lipoxygenase inhibitory, and proteinase inhibitory, measuring IC50 of 7.48 ± 0.48 µg/mL, 9.44 ± 1.62 µg/mL, 14.67 ± 1.94 µg/mL, and 28.57 ± 2.39 µg/mL, respectively. In conclusion, this study demonstrated the twelve leaf extracts’ significant antioxidant, antiarthritic, and anti-inflammatory activities. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2023 1 15 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Amit Kumar Shrivastava Muskan Keshari Manisha Neupane Sheshbhan Chaudhary Purna Kala Dhakal Laxmi Shrestha Anjan Palikhey Chandrajeet Kumar Yadav Gopal Lamichhane Mohammad Ujair Shekh Rakesh Kumar Yadav Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
This study aimed to evaluate the antioxidant, antiarthritic, and anti-inflammatory properties of extracts from the leaves of twelve different medicinal plants in Nepal. We then evaluated the total phenolic, flavonoid, and tannin contents of the extract using in-vitro assays and characterized it using GC-MS analysis. Results revealed that most of the leaf extracts contained phenolic compounds, flavonoids, tannins, alkaloids, and saponins. Few plants also showed the presence of glycosides, phytate, and vitamin C. Among the studied plants, Neolamarckia cadamba exhibited the highest total phenolic and tannin contents, as 241.53 ± 0.20 µg of gallic acid equivalent/mg and 74.48 ± 1.081 µg of tannic acid equivalent/mg, respectively. Ipomoea batatas exhibited the highest total flavonoid content, as 53.051 ± 1.11 µg of quercetin equivalent/mg. Moreover, Raphanus sativus demonstrated significant ferrous ion chelating, 2,2-diphenyl-1-picrylhydrazyl, hydrogen peroxide scavenging, and total antioxidant activities with IC50 value of 4.76 ± 0.68 µg/mL, 5.84 ± 0.14 µg/mL, 6.89 ± 0.16 µg/mL, and 8.99 ± 0.20 µg/mL, respectively. Similarly, Colocasia esculenta and Cicer arietinum exhibited the highest hydroxyl radical and nitric oxide scavenging activities, measuring IC50 value of 7.22 ± 0.56 µg/mL and 9.06 ± 0.10 µg/mL, respectively. Among all the extracts, Amorphophallus paeoniifolius displayed significant human red blood cell (HRBC) membrane stabilization activity (IC50 = 6.22 ± 0.78 µg/mL). Furthermore, Raphanus sativus, Chenopodium album, Cicer arietinum, and Murraya koenigii exhibited the highest inhibitory activities against protein denaturation with bovine serum albumin, antiarthritic, lipoxygenase inhibitory, and proteinase inhibitory, measuring IC50 of 7.48 ± 0.48 µg/mL, 9.44 ± 1.62 µg/mL, 14.67 ± 1.94 µg/mL, and 28.57 ± 2.39 µg/mL, respectively. In conclusion, this study demonstrated the twelve leaf extracts’ significant antioxidant, antiarthritic, and anti-inflammatory activities. |
format |
Article in Journal/Newspaper |
author |
Amit Kumar Shrivastava Muskan Keshari Manisha Neupane Sheshbhan Chaudhary Purna Kala Dhakal Laxmi Shrestha Anjan Palikhey Chandrajeet Kumar Yadav Gopal Lamichhane Mohammad Ujair Shekh Rakesh Kumar Yadav |
author_facet |
Amit Kumar Shrivastava Muskan Keshari Manisha Neupane Sheshbhan Chaudhary Purna Kala Dhakal Laxmi Shrestha Anjan Palikhey Chandrajeet Kumar Yadav Gopal Lamichhane Mohammad Ujair Shekh Rakesh Kumar Yadav |
author_sort |
Amit Kumar Shrivastava |
title |
Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal |
title_short |
Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal |
title_full |
Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal |
title_fullStr |
Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal |
title_full_unstemmed |
Evaluation of Antioxidant and Anti-Inflammatory Activities, and Metabolite Profiling of Selected Medicinal Plants of Nepal |
title_sort |
evaluation of antioxidant and anti-inflammatory activities, and metabolite profiling of selected medicinal plants of nepal |
publisher |
Hindawi Limited |
publishDate |
2023 |
url |
https://doi.org/10.1155/2023/6641018 https://doaj.org/article/201a036ffffe40f9a735e83ebfa95a71 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2023 (2023) |
op_relation |
http://dx.doi.org/10.1155/2023/6641018 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/6641018 https://doaj.org/article/201a036ffffe40f9a735e83ebfa95a71 |
op_doi |
https://doi.org/10.1155/2023/6641018 |
container_title |
Journal of Tropical Medicine |
container_volume |
2023 |
container_start_page |
1 |
op_container_end_page |
15 |
_version_ |
1784889565822058496 |