Zooshikella harenae sp. nov., Isolated from Pacific Oyster Crassostrea gigas , and Establishment of Zooshikella ganghwensis subsp. marina subsp. nov. and Zooshikella ganghwensis subsp. ganghwensis subsp. nov.

• Published in: Diversity
• Main Authors: Hani Pira, Chandra Risdian, Peter Kämpfer, Mathias Müsken, Peter J. Schupp, Joachim Wink
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2021
• Subjects: pacific oyster Crassostrea gigas; polyphasic taxonomy; Wilhelmshaven Sea; Zooshikella; Biology (General); QH301-705.5; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://doi.org/10.3390/d13120641; https://doaj.org/article/1fe773a63d1c40ab8a5a77d1cff3f50c

Here, we describe the polyphasic taxonomy of a novel isolated strain WH53 T from the genus Zooshikella isolated from the sand sediment located between the lumen of the Crassostrea gigas From Germany. Phylogenetic analysis determined that the strain WH53 T had a high similarity to Zooshikella ganghwensis JC2044 T (99.57%) and Zooshikella marina LMG 28823 T (99.36%). Strain WH53 T contained ubiquinone-9 (Q-9) as the predominant menaquinone, and the major fatty acids were C 16:0 , C 16:1 ω7c, and C 18:1 ω7c. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, amino phospholipids, and unidentified phospholipids were identified as their polar lipid composition. The DNA G+C content and genome size of strain WH53 T were 40.08 mol% and 5,914,969 bp, respectively. Digital DNA–DNA hybridisation (dDDH) for strain WH53 T against Z. ganghwensis JC2044 T and Z. marina LMG 28823 T showed low relatedness values of 26.3% and 26.1%, respectively. The extract of strain WH53 T exhibited antimicrobial property. Strain WH53 T represents a novel species in the genus Zooshikella . We propose the name of Zooshikella harenae sp. nov., with the type strain WH53 T (= DSM 111628 T = NCCB 100808 T ). Furthermore, the dDDH, average nucleotide identity (ANI), percentage of conserved proteins (POCP), and amino acid identity (AAI) value between Z. marina LGM 28823 T and Z. ganghwensis DSM 15267 T were 79.9%, 97.84%, 76.08%, and 87.01%, respectively, suggesting that both of them should be reclassified as Z. ganghwensis subsp. marina subsp. nov. and Z. ganghwensis subsp. ganghwensis DSM 15267 subsp. nov.