Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation
The genetic structure and phenotypic diversity of two populations of Cheilosia aff. longula (Diptera: Syrphidae) in Lapland, Finland, were examined using DNA sequencing, protein electrophoresis, and geometric morphometrics. The morphological identification of the species were verified using partial...
Published in: | European Journal of Entomology |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2010
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ftdoajarticles:oai:doaj.org/article:1f5f37ada4c04d13bef21081cdfbef12 2023-05-15T18:42:29+02:00 Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation Vesna MILANKOV Ljubinka FRANCUSKI Jasmina LUDOŠKI Gunilla STÅHLS Ante VUJIĆ 2010-07-01T00:00:00Z https://doi.org/10.14411/eje.2010.039 https://doaj.org/article/1f5f37ada4c04d13bef21081cdfbef12 EN eng Institute of Entomology, Biology Centre, Czech Academy of Science https://www.eje.cz/artkey/eje-201003-0003_Genetic_structure_and_phenotypic_diversity_of_two_northern_populations_of_Cheilosia_aff_longula_Diptera_Syrp.php https://doaj.org/toc/1210-5759 https://doaj.org/toc/1802-8829 1210-5759 1802-8829 doi:10.14411/eje.2010.039 https://doaj.org/article/1f5f37ada4c04d13bef21081cdfbef12 European Journal of Entomology, Vol 107, Iss 3, Pp 305-315 (2010) diptera syrphidae cheilosia aff. longula allozymes asymmetry coi mtdna genetic diversity its2 rdna wing geometric morphometrics Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.14411/eje.2010.039 2022-12-31T10:34:28Z The genetic structure and phenotypic diversity of two populations of Cheilosia aff. longula (Diptera: Syrphidae) in Lapland, Finland, were examined using DNA sequencing, protein electrophoresis, and geometric morphometrics. The morphological identification of the species were verified using partial sequences of mitochondrial cytochrome c oxidase subunit I (COI mtDNA), and the nuclear ribosomal internal transcribed region 2 (ITS2 rDNA), and comparing the corresponding sequences of Cheilosia aff. longula and the closely related C. longula. Two and three haplotypes of the genes COI mtDNA and ITS2 rDNA were identified in the two populations. Analysis of 12 isozyme loci showed an extremely low genetic variability in the populations originating from Utsjoki and Kevo. Discriminant analysis combined with canonical variate analysis revealed inter-population divergence in wing shape. Variation among genetically diverse individuals, both within- and among studied populations was studied, and directional (DA) and fluctuating asymmetry (FA) estimated using landmarks in the framework of geometric morphometrics. It is likely that the documented DA and FA asymmetry in both wing shape and size reflects the developmental instability of the individuals studied. By using Procrustes ANOVA the locations of particular landmarks responsible for the variation in shape were determined. The decomposition of the components of variance accorded to each landmark showed that the landmarks differed in the percentage of variation they accounted for (DA, FA and variation among individuals). In the discussion the implications of the reduced genetic diversity and asymmetry in wing traits for conservation is considered. Article in Journal/Newspaper Utsjoki Lapland Directory of Open Access Journals: DOAJ Articles Kevo ENVELOPE(27.020,27.020,69.758,69.758) European Journal of Entomology 107 3 305 315 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
diptera syrphidae cheilosia aff. longula allozymes asymmetry coi mtdna genetic diversity its2 rdna wing geometric morphometrics Zoology QL1-991 |
spellingShingle |
diptera syrphidae cheilosia aff. longula allozymes asymmetry coi mtdna genetic diversity its2 rdna wing geometric morphometrics Zoology QL1-991 Vesna MILANKOV Ljubinka FRANCUSKI Jasmina LUDOŠKI Gunilla STÅHLS Ante VUJIĆ Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation |
topic_facet |
diptera syrphidae cheilosia aff. longula allozymes asymmetry coi mtdna genetic diversity its2 rdna wing geometric morphometrics Zoology QL1-991 |
description |
The genetic structure and phenotypic diversity of two populations of Cheilosia aff. longula (Diptera: Syrphidae) in Lapland, Finland, were examined using DNA sequencing, protein electrophoresis, and geometric morphometrics. The morphological identification of the species were verified using partial sequences of mitochondrial cytochrome c oxidase subunit I (COI mtDNA), and the nuclear ribosomal internal transcribed region 2 (ITS2 rDNA), and comparing the corresponding sequences of Cheilosia aff. longula and the closely related C. longula. Two and three haplotypes of the genes COI mtDNA and ITS2 rDNA were identified in the two populations. Analysis of 12 isozyme loci showed an extremely low genetic variability in the populations originating from Utsjoki and Kevo. Discriminant analysis combined with canonical variate analysis revealed inter-population divergence in wing shape. Variation among genetically diverse individuals, both within- and among studied populations was studied, and directional (DA) and fluctuating asymmetry (FA) estimated using landmarks in the framework of geometric morphometrics. It is likely that the documented DA and FA asymmetry in both wing shape and size reflects the developmental instability of the individuals studied. By using Procrustes ANOVA the locations of particular landmarks responsible for the variation in shape were determined. The decomposition of the components of variance accorded to each landmark showed that the landmarks differed in the percentage of variation they accounted for (DA, FA and variation among individuals). In the discussion the implications of the reduced genetic diversity and asymmetry in wing traits for conservation is considered. |
format |
Article in Journal/Newspaper |
author |
Vesna MILANKOV Ljubinka FRANCUSKI Jasmina LUDOŠKI Gunilla STÅHLS Ante VUJIĆ |
author_facet |
Vesna MILANKOV Ljubinka FRANCUSKI Jasmina LUDOŠKI Gunilla STÅHLS Ante VUJIĆ |
author_sort |
Vesna MILANKOV |
title |
Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation |
title_short |
Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation |
title_full |
Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation |
title_fullStr |
Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation |
title_full_unstemmed |
Genetic structure and phenotypic diversity of two northern populations of Cheilosia aff. longula (Diptera: Syrphidae) has implications for evolution and conservation |
title_sort |
genetic structure and phenotypic diversity of two northern populations of cheilosia aff. longula (diptera: syrphidae) has implications for evolution and conservation |
publisher |
Institute of Entomology, Biology Centre, Czech Academy of Science |
publishDate |
2010 |
url |
https://doi.org/10.14411/eje.2010.039 https://doaj.org/article/1f5f37ada4c04d13bef21081cdfbef12 |
long_lat |
ENVELOPE(27.020,27.020,69.758,69.758) |
geographic |
Kevo |
geographic_facet |
Kevo |
genre |
Utsjoki Lapland |
genre_facet |
Utsjoki Lapland |
op_source |
European Journal of Entomology, Vol 107, Iss 3, Pp 305-315 (2010) |
op_relation |
https://www.eje.cz/artkey/eje-201003-0003_Genetic_structure_and_phenotypic_diversity_of_two_northern_populations_of_Cheilosia_aff_longula_Diptera_Syrp.php https://doaj.org/toc/1210-5759 https://doaj.org/toc/1802-8829 1210-5759 1802-8829 doi:10.14411/eje.2010.039 https://doaj.org/article/1f5f37ada4c04d13bef21081cdfbef12 |
op_doi |
https://doi.org/10.14411/eje.2010.039 |
container_title |
European Journal of Entomology |
container_volume |
107 |
container_issue |
3 |
container_start_page |
305 |
op_container_end_page |
315 |
_version_ |
1766232136632762368 |