Evaluation of fecal immunoassays for canine Echinococcus infection in China.
Human echinococcosis is present worldwide but it is in China that disease prevalence is the highest. In western China, especially in the Tibetan Plateau, the burden of echinococcosis is the most important. Dogs are a major definitive host of Echinococcus and monitoring the presence of Echinococcus w...
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Online Access: | https://doi.org/10.1371/journal.pntd.0008690 https://doaj.org/article/1f1d88dd4d434a53a8c8f1b723aa3361 |
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ftdoajarticles:oai:doaj.org/article:1f1d88dd4d434a53a8c8f1b723aa3361 2023-05-15T15:06:17+02:00 Evaluation of fecal immunoassays for canine Echinococcus infection in China. Liying Wang Qian Wang Huixia Cai Hu Wang Yan Huang Yu Feng Xuefei Bai Min Qin Sylvie Manguin Laurent Gavotte Weiping Wu Roger Frutos 2021-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008690 https://doaj.org/article/1f1d88dd4d434a53a8c8f1b723aa3361 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008690 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008690 https://doaj.org/article/1f1d88dd4d434a53a8c8f1b723aa3361 PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0008690 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008690 2022-12-31T08:01:18Z Human echinococcosis is present worldwide but it is in China that disease prevalence is the highest. In western China, especially in the Tibetan Plateau, the burden of echinococcosis is the most important. Dogs are a major definitive host of Echinococcus and monitoring the presence of Echinococcus worms in dogs is therefore essential to efficiently control the disease. Detection kits based on three different technologies including sandwich ELISA, (indirect) ELISA, and gold immunodiffusion, are currently marketed and used in China. The objective of this work was to assess the efficacy of these kits, in particular with respect to sensitivity and specificity. Four fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. The results indicate that the performance is lower than expected, in particular in terms of sensitivity. The best results were obtained with the sandwich ELISA technology. The gold immunofiltration yielded the poorest results. In all cases, further development is needed to improve the performance of these kits which are key components for the control of echinococcosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 3 e0008690 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Liying Wang Qian Wang Huixia Cai Hu Wang Yan Huang Yu Feng Xuefei Bai Min Qin Sylvie Manguin Laurent Gavotte Weiping Wu Roger Frutos Evaluation of fecal immunoassays for canine Echinococcus infection in China. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Human echinococcosis is present worldwide but it is in China that disease prevalence is the highest. In western China, especially in the Tibetan Plateau, the burden of echinococcosis is the most important. Dogs are a major definitive host of Echinococcus and monitoring the presence of Echinococcus worms in dogs is therefore essential to efficiently control the disease. Detection kits based on three different technologies including sandwich ELISA, (indirect) ELISA, and gold immunodiffusion, are currently marketed and used in China. The objective of this work was to assess the efficacy of these kits, in particular with respect to sensitivity and specificity. Four fecal antigen detection kits for canine infection reflecting the three technologies were obtained from companies and tested in parallel on 220 fecal samples. The results indicate that the performance is lower than expected, in particular in terms of sensitivity. The best results were obtained with the sandwich ELISA technology. The gold immunofiltration yielded the poorest results. In all cases, further development is needed to improve the performance of these kits which are key components for the control of echinococcosis. |
format |
Article in Journal/Newspaper |
author |
Liying Wang Qian Wang Huixia Cai Hu Wang Yan Huang Yu Feng Xuefei Bai Min Qin Sylvie Manguin Laurent Gavotte Weiping Wu Roger Frutos |
author_facet |
Liying Wang Qian Wang Huixia Cai Hu Wang Yan Huang Yu Feng Xuefei Bai Min Qin Sylvie Manguin Laurent Gavotte Weiping Wu Roger Frutos |
author_sort |
Liying Wang |
title |
Evaluation of fecal immunoassays for canine Echinococcus infection in China. |
title_short |
Evaluation of fecal immunoassays for canine Echinococcus infection in China. |
title_full |
Evaluation of fecal immunoassays for canine Echinococcus infection in China. |
title_fullStr |
Evaluation of fecal immunoassays for canine Echinococcus infection in China. |
title_full_unstemmed |
Evaluation of fecal immunoassays for canine Echinococcus infection in China. |
title_sort |
evaluation of fecal immunoassays for canine echinococcus infection in china. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0008690 https://doaj.org/article/1f1d88dd4d434a53a8c8f1b723aa3361 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0008690 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008690 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008690 https://doaj.org/article/1f1d88dd4d434a53a8c8f1b723aa3361 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008690 |
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PLOS Neglected Tropical Diseases |
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15 |
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3 |
container_start_page |
e0008690 |
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1766337917226057728 |