Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan
Abstract Background Scarce data are available on Plasmodium falciparum anti-malarial drug resistance in Pakistan. The aim of this study was, therefore, to determine the prevalence of P. falciparum resistance associated polymorphisms in field isolates from southern Pakistan. Methods Blood samples fro...
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ftdoajarticles:oai:doaj.org/article:1f19b3d107ca4773a835375b4a4cdfbf 2023-05-15T15:08:04+02:00 Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan Beg Mohammad A Veiga Maria I Ursing Johan Ghanchi Najia Jafri Sana Mårtensson Andreas 2011-01-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-18 https://doaj.org/article/1f19b3d107ca4773a835375b4a4cdfbf EN eng BMC http://www.malariajournal.com/content/10/1/18 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-18 1475-2875 https://doaj.org/article/1f19b3d107ca4773a835375b4a4cdfbf Malaria Journal, Vol 10, Iss 1, p 18 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-18 2022-12-31T00:10:07Z Abstract Background Scarce data are available on Plasmodium falciparum anti-malarial drug resistance in Pakistan. The aim of this study was, therefore, to determine the prevalence of P. falciparum resistance associated polymorphisms in field isolates from southern Pakistan. Methods Blood samples from 244 patients with blood-slide confirmed P. falciparum mono-infections were collected between 2005-2007. Single nucleotide polymorphisms in the P. falciparum chloroquine resistance transporter ( pfcrt K76T), multi drug resistance ( pfmdr1 N86Y), dihydrofolate reductase ( pfdhfr A16V, N51I, C59R, S108N, I164L) and dihydropteroate synthetase ( pfdhps A436S, G437A and E540K) genes and pfmdr1 gene copy numbers were determined using PCR based methods. Results The prevalence of pfcrt 76T and pfmdr1 86Y was 93% and 57%, respectively. The prevalence of pfdhfr double mutations 59R + 108N/51R + 108N was 92%. The pfdhfr triple mutation (51I, 59R, 108N) occurred in 3% of samples. The pfdhfr (51I, 59R, 108N) and pfdhps (437G, 540E) quintuple mutation was found in one isolate. Pfdhps 437G was observed in 51% and 540E in 1% of the isolates. One isolate had two pfmdr1 copies and carried the pfmdr1 86Y and pfcrt 76T alleles. Conclusions The results indicate high prevalence of in vivo resistance to chloroquine, whereas high grade resistance to sulphadoxine-pyrimethamine does not appear to be widespread among P. falciparum in southern Pakistan. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 18 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Beg Mohammad A Veiga Maria I Ursing Johan Ghanchi Najia Jafri Sana Mårtensson Andreas Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Scarce data are available on Plasmodium falciparum anti-malarial drug resistance in Pakistan. The aim of this study was, therefore, to determine the prevalence of P. falciparum resistance associated polymorphisms in field isolates from southern Pakistan. Methods Blood samples from 244 patients with blood-slide confirmed P. falciparum mono-infections were collected between 2005-2007. Single nucleotide polymorphisms in the P. falciparum chloroquine resistance transporter ( pfcrt K76T), multi drug resistance ( pfmdr1 N86Y), dihydrofolate reductase ( pfdhfr A16V, N51I, C59R, S108N, I164L) and dihydropteroate synthetase ( pfdhps A436S, G437A and E540K) genes and pfmdr1 gene copy numbers were determined using PCR based methods. Results The prevalence of pfcrt 76T and pfmdr1 86Y was 93% and 57%, respectively. The prevalence of pfdhfr double mutations 59R + 108N/51R + 108N was 92%. The pfdhfr triple mutation (51I, 59R, 108N) occurred in 3% of samples. The pfdhfr (51I, 59R, 108N) and pfdhps (437G, 540E) quintuple mutation was found in one isolate. Pfdhps 437G was observed in 51% and 540E in 1% of the isolates. One isolate had two pfmdr1 copies and carried the pfmdr1 86Y and pfcrt 76T alleles. Conclusions The results indicate high prevalence of in vivo resistance to chloroquine, whereas high grade resistance to sulphadoxine-pyrimethamine does not appear to be widespread among P. falciparum in southern Pakistan. |
format |
Article in Journal/Newspaper |
author |
Beg Mohammad A Veiga Maria I Ursing Johan Ghanchi Najia Jafri Sana Mårtensson Andreas |
author_facet |
Beg Mohammad A Veiga Maria I Ursing Johan Ghanchi Najia Jafri Sana Mårtensson Andreas |
author_sort |
Beg Mohammad A |
title |
Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan |
title_short |
Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan |
title_full |
Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan |
title_fullStr |
Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan |
title_full_unstemmed |
Prevalence of resistance associated polymorphisms in Plasmodium falciparum field isolates from southern Pakistan |
title_sort |
prevalence of resistance associated polymorphisms in plasmodium falciparum field isolates from southern pakistan |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-18 https://doaj.org/article/1f19b3d107ca4773a835375b4a4cdfbf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 18 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/18 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-18 1475-2875 https://doaj.org/article/1f19b3d107ca4773a835375b4a4cdfbf |
op_doi |
https://doi.org/10.1186/1475-2875-10-18 |
container_title |
Malaria Journal |
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10 |
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1 |
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18 |
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1766339491547578368 |