Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania
In Tasmania, Australian fur seals ( Arctocephalus pusillus doriferus ) regularly interact with Atlantic salmon ( Salmo salmar L.) aquaculture lease operations and opportunistically consume fish. The microbial communities of seals and aquaculture salmon were analyzed for potential indicators of micro...
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2023
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Online Access: | https://doi.org/10.3390/oceans4020014 https://doaj.org/article/1f16975297334c629f7af42ca232bb03 |
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ftdoajarticles:oai:doaj.org/article:1f16975297334c629f7af42ca232bb03 2023-07-23T04:18:23+02:00 Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania Erin D’Agnese Ryan J. McLaughlin Mary-Anne Lea Esteban Soto Woutrina A. Smith John P. Bowman 2023-06-01T00:00:00Z https://doi.org/10.3390/oceans4020014 https://doaj.org/article/1f16975297334c629f7af42ca232bb03 EN eng MDPI AG https://www.mdpi.com/2673-1924/4/2/14 https://doaj.org/toc/2673-1924 doi:10.3390/oceans4020014 2673-1924 https://doaj.org/article/1f16975297334c629f7af42ca232bb03 Oceans, Vol 4, Iss 14, Pp 200-219 (2023) Australian fur seal microbiome aquaculture Tasmania One Health microbial source tracking Oceanography GC1-1581 article 2023 ftdoajarticles https://doi.org/10.3390/oceans4020014 2023-07-02T00:37:26Z In Tasmania, Australian fur seals ( Arctocephalus pusillus doriferus ) regularly interact with Atlantic salmon ( Salmo salmar L.) aquaculture lease operations and opportunistically consume fish. The microbial communities of seals and aquaculture salmon were analyzed for potential indicators of microbial sharing and to determine the potential effects of interactions on wild seal microbiome composition. The high-throughput sequencing of the V1–V3 region of the 16S rRNA genes from the gut microbial communities of 221 fur seals was performed: 41 males caught at farms, 50 adult scats from haul-outs near farms, 24 necropsied seals, and controls from Bass Strait breeding colonies, encompassing 56 adult scats and 50 pup swabs. QIIME2 and R Studio were used for analysis. Foraging at or near salmon farms significantly shifted seal microbiome biodiversity. Taxonomic analysis showed a greater divergence in Bacteroidota representatives in male seals captured at farms compared to all other groups. Pathogens were identified that could be monitoring targets. Potential indicator amplicon sequence variants were found across a variety of taxa and could be used as minimally invasive indicators for interactions at this interface. The diversity and taxonomic shifts in the microbial communities of seals indicate a need to further study this interface for broader ecological implications. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Oceans 4 2 200 219 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Australian fur seal microbiome aquaculture Tasmania One Health microbial source tracking Oceanography GC1-1581 |
spellingShingle |
Australian fur seal microbiome aquaculture Tasmania One Health microbial source tracking Oceanography GC1-1581 Erin D’Agnese Ryan J. McLaughlin Mary-Anne Lea Esteban Soto Woutrina A. Smith John P. Bowman Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania |
topic_facet |
Australian fur seal microbiome aquaculture Tasmania One Health microbial source tracking Oceanography GC1-1581 |
description |
In Tasmania, Australian fur seals ( Arctocephalus pusillus doriferus ) regularly interact with Atlantic salmon ( Salmo salmar L.) aquaculture lease operations and opportunistically consume fish. The microbial communities of seals and aquaculture salmon were analyzed for potential indicators of microbial sharing and to determine the potential effects of interactions on wild seal microbiome composition. The high-throughput sequencing of the V1–V3 region of the 16S rRNA genes from the gut microbial communities of 221 fur seals was performed: 41 males caught at farms, 50 adult scats from haul-outs near farms, 24 necropsied seals, and controls from Bass Strait breeding colonies, encompassing 56 adult scats and 50 pup swabs. QIIME2 and R Studio were used for analysis. Foraging at or near salmon farms significantly shifted seal microbiome biodiversity. Taxonomic analysis showed a greater divergence in Bacteroidota representatives in male seals captured at farms compared to all other groups. Pathogens were identified that could be monitoring targets. Potential indicator amplicon sequence variants were found across a variety of taxa and could be used as minimally invasive indicators for interactions at this interface. The diversity and taxonomic shifts in the microbial communities of seals indicate a need to further study this interface for broader ecological implications. |
format |
Article in Journal/Newspaper |
author |
Erin D’Agnese Ryan J. McLaughlin Mary-Anne Lea Esteban Soto Woutrina A. Smith John P. Bowman |
author_facet |
Erin D’Agnese Ryan J. McLaughlin Mary-Anne Lea Esteban Soto Woutrina A. Smith John P. Bowman |
author_sort |
Erin D’Agnese |
title |
Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania |
title_short |
Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania |
title_full |
Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania |
title_fullStr |
Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania |
title_full_unstemmed |
Comparative Microbial Community Analysis of Fur Seals and Aquaculture Salmon Gut Microbiomes in Tasmania |
title_sort |
comparative microbial community analysis of fur seals and aquaculture salmon gut microbiomes in tasmania |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/oceans4020014 https://doaj.org/article/1f16975297334c629f7af42ca232bb03 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Oceans, Vol 4, Iss 14, Pp 200-219 (2023) |
op_relation |
https://www.mdpi.com/2673-1924/4/2/14 https://doaj.org/toc/2673-1924 doi:10.3390/oceans4020014 2673-1924 https://doaj.org/article/1f16975297334c629f7af42ca232bb03 |
op_doi |
https://doi.org/10.3390/oceans4020014 |
container_title |
Oceans |
container_volume |
4 |
container_issue |
2 |
container_start_page |
200 |
op_container_end_page |
219 |
_version_ |
1772180686454652928 |